<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; color: rgb(0, 0, 0); font-size: 14px; "><div><div><div style="font-family: Calibri, sans-serif; "><span class="Apple-style-span" style="font-family: Calibri; "><div style="font-family: Calibri, sans-serif; ">Hi </div><div style="font-family: Calibri, sans-serif; "><br></div><div style="font-family: Calibri, sans-serif; ">I have run maker and some of the protein predicted by maker does not start with a Methionine. I am not sure why</div><div style="font-family: Calibri, sans-serif; "><br></div><div style="font-family: Calibri, sans-serif; ">Here are some examples</div><div style="font-family: Calibri, sans-serif; "><br></div><div style="font-family: Calibri, sans-serif; "><div>>maker-scaffold00001-snap-gene-0.8-mRNA-1 protein AED:0.27496328928047 eAED:0.27496328928047 QI:0|0.4|0.16|0.5|1|1|6|0|453</div><div>VIIKFKTFAKASRSVELFGHEGAWARGDGYCNFKTESEKADRSVKSSCSLNIPFTYDVGR</div><div>RQYVIKGDRFCLSHNHLVMIPSPTTVIVNDQRDLTPDQLSYIINLGKYSLPFPMVTRMLS</div><div>DQFPDCRIQKPLLHRLLRKGKLQAFGGDRDAMNALINLGRSYEEHGGFFEIDIDVDCRLE</div><div>KIWLARAEGLQFASVYNDVVQIDGGAKMNAYGFVFLPVTVIDCLGKSYVVGAMAGPSAEN</div><div>KADVVKTLEYFRVKRSESVLIADDALAFRAAAVECDMVYHQCTKHYQAKIARACAGLGHE</div><div>GKEFMIKANTLVYHIFPSEDAFFAKADEYRLMFLQYGGAVKLFDDIVDKRQQLCRTFTSC</div><div>KFTGSHSSNQRAEGTISRTKRDVQPWLSRANLFEMFTHLEMIQKQQEDEAARLLSNLIRK</div><div>GKHWSDYVDSIFRERQLNSRLLSSVREVDTGLH</div></div><div style="font-family: Calibri, sans-serif; "><br></div><div style="font-family: Calibri, sans-serif; "><div>>snap-scaffold00087-abinit-gene-2.145-mRNA-1 protein AED:0.0539495114006514 eAED:0.0539495114006514 QI:2|1|0.6|1|1|1|5|0|817</div><div>ALSLHGTRQAFARVPPPCRRAHPAERRQPGGGMSADAPVKAGYLLKLTSSLSHWNRRYFI</div><div>VADTKLFYCKTEDDLLRRKFQGEIDLAGAQIALYTRNDETAKRFSDHHHMLGVKPAGCDR</div><div>IYILDADSEHAQKEWVACLRRHASQAPVSSPVDAAVAAAPRKDPQSVREGFLTKRGETIK</div><div>NWKMRYFVLKGNYLHYYRSIEDAQPAGSILLLGTRTTAEPKAVTGMPHSFSIARADAKRK</div><div>YMIHADSKEECDAWVGAIQQQSVFVRHAGTDSAPPEVVAPAAATPVHQQHQSRSSFGNRP</div><div>NVADDSADDDEAALDEVALSNGPPALAPHGIANTGASTGLNLKQKVSKKKRRFVTDEFDL</div><div>DLTYITENIIAMGFPAESMEAMFRNSMSDVQRFLDGRHPDAYRVYNLCSERDYDPAKFHH</div><div>NVCRFPFDDHNCPNFEDLIPLCEDIHNWLSIQSDHVVAIHCKAGKGRTGLVICAYLLYSG</div><div>AWRTARDALQFYGFVRTQDQKGVTIPSQIRYVEYFEQYMADPEILSRNNGPLVISEIFVG</div><div>RGCRPFDTVTITNMGRRMNSKDWGKYWKDALDDGLLLQLPKGACQVDKDFKVEFLASGLL</div><div>GKKTRVAGFWLHTAFIQDGVVDIDKSMIDKVNKEKDCPAFSIQVFFGGRTYVDRRCRIPV</div><div>APPQPTGPLLLSPATVRIRNADPLPVPNPSSPSESPSFSAMSSVPSLSLESLSSVSSSLS</div><div>PTTGPKAAPSPKKQDPGLDPGSPPGTVKSAPVAEAGAAPVDARSDNKAPARSCSLPHGRF</div><div>PGDVAGDGAFQQAAVQVRIAFWNNLQSEALQRRNSRL</div></div><div style="font-family: Calibri, sans-serif; "><br></div><div style="font-family: Calibri, sans-serif; "><div>>augustus-scaffold00087-abinit-gene-0.106-mRNA-1 protein AED:0.10935424621144 eAED:0.10935424621144 QI:1|1|0.66|1|1|1|3|0|483</div><div>STSTVFCPGLWNRVGRYVSGGLQTSPVTVPRQVRPICLATQPATDQPGYVMSSTSALVGI</div><div>GVVTVALLCRWAPILVTTSGPGSPARSSADVMRIWADHDWSAGTTSLPISQESLLAKRVL</div><div>SKSFDGLPPNLHVQDDTVPVALLRAHLNAGRHMRLRDMCPTAGACDLQGADPDHGLAPLH</div><div>MAAMRDDRSSIAYLMALGADPDAMDRAGRQYRNLSFTNFVRNARRAAEERGSTCQLPEVN</div><div>LAGLERADLDRSWAEIRRLAHEGEPVAIRGLLGAYDRSDVLDWDLDAFLTRHGHVPVNVG</div><div>DVPYAQYFGLPIQSMPLSKYVASLAPGSASYVFAKDDGICRDALQILDRFARDALPPYFV</div><div>SPAALGSDAVHFYLGNKGSGAPFHLHSDAVNLLAHGSKTWFVTPPPQSVYSRTPIGEFAA</div><div>NGTSGIESLRCEQNPGDAIYIPFDWGHAVLNNEDSTFGFAVELLNKRDSLHFLRPSSQVP</div><div>AGQ</div></div><div style="font-family: Calibri, sans-serif; "><br></div><div style="font-family: Calibri, sans-serif; "><br></div><div style="font-family: Calibri, sans-serif; ">Regards</div><div style="font-family: Calibri, sans-serif; "><br></div><div style="font-family: Calibri, sans-serif; ">Hossein</div></span></div><div><div style="color: rgb(0, 0, 0); font-size: 14px; "><br></div><div style="font-family: Calibri, sans-serif; "><p class="MsoNormal" style="font-family: Calibri, sans-serif; font-size: 14px; color: rgb(0, 0, 0); margin-top: 0cm; margin-right: 0cm; margin-left: 0cm; margin-bottom: 0.0001pt; "><font class="Apple-style-span" face="Arial,sans-serif"><span class="Apple-style-span" style="font-size: 13px;"><br></span></font></p><p class="MsoNormal" style="font-family: Calibri, sans-serif; font-size: 14px; color: rgb(0, 0, 0); margin-top: 0cm; margin-right: 0cm; margin-left: 0cm; margin-bottom: 0.0001pt; "><font class="Apple-style-span" face="Arial,sans-serif"><span class="Apple-style-span" style="font-size: 13px;"><br></span></font></p><p class="MsoNormal" style="font-family: Calibri, sans-serif; font-size: 14px; color: rgb(0, 0, 0); margin-top: 0cm; margin-right: 0cm; margin-left: 0cm; margin-bottom: 0.0001pt; "><font class="Apple-style-span" face="Arial,sans-serif"><span class="Apple-style-span" style="font-size: 13px;"><br></span></font></p></div></div></div></div></body></html>