<html><head><meta http-equiv="Content-Type" content="text/html charset=iso-8859-1"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hello,<div>I am trying to annotation a Chlamydomonas genome and C. reinhartii was used as a model organism in Augustus. I would like to add this model to augustus_species in the maker_opts.ctl file, but I'm not sure how this information should be inserted on this line (ie. as genus name, file location, etc).</div><div><br></div><div>I am also having an issue with providing a protein file. When I put in the protein fasta file of C. reinhartti from the Augustus website, I get a fatal error (below). I've looked through the fasta and I'm not seeing anything obvious that would cause this error to be thrown. Do you have any suggestions on where to start to look? </div><div><br></div><div><br></div><div><div>Can't open sequence index file /Users/kara/Desktop/CBMW_maker_protein/contigs.maker.output/mpi_blastdb/augustus%2Eu9_aa%2Efasta.mpi.10/augustus%2Eu9_aa%2Efasta.mpi.10.1.index: Inappropriate file type or format at /sw/lib/perl5/5.12.4/Bio/DB/Fasta.pm line 527.</div><div><br></div><div>FATAL ERROR</div></div><div><br></div><div><br></div><div>Thanks for any help you can provide.</div><div><br></div><div>Kara</div><div><br></div><div><br></div><div apple-content-edited="true">
<div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~</div><div>Kara De León</div><div>Postdoctoral Research Associate</div><div>Montana State University</div><div>Center for Biofilm Engineering</div><div>366 EPS Building</div><div>Bozeman, MT 59717</div><div>208-484-9078</div><div><a href="mailto:kara.deleon@biofilm.montana.edu">kara.deleon@biofilm.montana.edu</a></div><div>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~</div><div><br></div></div><br class="Apple-interchange-newline"></div><br class="Apple-interchange-newline"></div><br class="Apple-interchange-newline"><br class="Apple-interchange-newline">
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