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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Hi Barry,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Thank you very much for the message and suggestions. It’s really helpful to us. We will do some tests to see how we can take advantage of Maker2 to produce
high-quality gene models from our assembled genome.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Thank you again,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Xia<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> Barry Moore [mailto:barry.moore@genetics.utah.edu]
<br>
<b>Sent:</b> Wednesday, October 02, 2013 1:21 PM<br>
<b>To:</b> CAO, XIA<br>
<b>Cc:</b> carsonhh@gmail.com; maker-devel@yandell-lab.org; RIVERA, CORBAN GREGORY<br>
<b>Subject:</b> Re: [maker-devel] maker2 scripts for functional annotation<o:p></o:p></span></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Hi Xia,<o:p></o:p></p>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">You can use EST evidence from other strains/species. The considerations are as follows: When you pass EST (or mRNASeq based transcripts) evidence MAKER uses blastn to align those with alignment parameters that assume they sequences will
align easily with almost perfect identity - this is fast. If you use EST/mRNA evidence from another species MAKER uses tblastx to compare the RNA evidence to the assembly, both translated into protein space - this works fine, but it is slow and often doesn't
get you much more evidence than you could have gotten from proteins as evidence. If you believe that you're other strain should have very little divergence in sequence at the nt level then you could experiment with passing EST/mRNA evidence as est in the
control file. Consider this an experiment, do it on a few contigs and examine the results carefully and skeptically. If it works well, that would be the way to go because you've get better UTRs, but if it doesn't you'll see that you get little support for
models because sequence divergence was too much for good alignment. In that case you'll need to use alt_est and this will be slower and you'll likely get little in the way of support for UTRs - in this case as well, do a few contigs and examine the output
carefully to see if the additional alignment time with tblastx is worth it in your case.<o:p></o:p></p>
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<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">Barry<o:p></o:p></p>
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<div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
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<div>
<p class="MsoNormal"><o:p> </o:p></p>
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<div>
<p class="MsoNormal">On Oct 2, 2013, at 8:40 AM, <<a href="mailto:Xia.Cao@dupont.com">Xia.Cao@dupont.com</a>><o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"> wrote:<o:p></o:p></p>
</div>
<p class="MsoNormal"><br>
<br>
<o:p></o:p></p>
<div>
<p class="MsoNormal">Hi Carson,<br>
<br>
Thank you for your quick and kind reply. One more question here. If we don't have EST evidence for the strain we sequenced/assembled, will it be ok to provide some EST evidence that is from some other strains which are close to the one we are trying to annotate?
Could you please let us know how this will affect performance of maker2?<br>
<br>
Thank you again.<br>
<br>
Best,<br>
Xia<br>
<br>
-----Original Message-----<br>
From: Carson Holt [<a href="mailto:carsonhh@gmail.com">mailto:carsonhh@gmail.com</a>]
<br>
Sent: Wednesday, October 02, 2013 10:15 AM<br>
To: CAO, XIA<br>
Cc: <a href="mailto:myandell@genetics.utah.edu">myandell@genetics.utah.edu</a>; RIVERA, CORBAN GREGORY;
<a href="mailto:maker-devel@yandell-lab.org">maker-devel@yandell-lab.org</a><br>
Subject: Re: [maker-devel] maker2 scripts for functional annotation<br>
<br>
It still works, but it will be reduced. Without ESTs you won't get any UTR prediction, also you will be limited by how well the protein set matches to the genes. If you use the protein set of a close relative you will capture most things. You will probably
capture 80-95% of what you would by also including ESTs. It all depending on how different the species in the proteins evidence file are compared to the the species being annotated.<br>
<br>
--Carson<br>
<br>
On 10/1/13 3:00 PM, "<a href="mailto:Xia.Cao@dupont.com">Xia.Cao@dupont.com</a>" <<a href="mailto:Xia.Cao@dupont.com">Xia.Cao@dupont.com</a>> wrote:<br>
<br>
<br>
<o:p></o:p></p>
<p class="MsoNormal">Hi Carson,<o:p></o:p></p>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Thank you for your message and your kind help. Now conversion from
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">match/match_part to gene/mRNA/exons/CDS works well after blanking out
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">some options in control file.<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">I have one more question about maker2. In case we don't have EST
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">evidence (set of ESTs or assembled mRNA-seq in fasta format) for a
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">genome, does<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">maker2 function? If it does function, could you please let me know the
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">performance of maker2 without providing EST evidence compared to the
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">one with EST evidence?<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Thank you again and I look forward to hearing from you.<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Best,<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Xia<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">-----Original Message-----<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">From: Carson Holt [<a href="mailto:carsonhh@gmail.com">mailto:carsonhh@gmail.com</a>]<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Sent: Wednesday, September 25, 2013 10:36 AM<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">To: CAO, XIA; <a href="mailto:myandell@genetics.utah.edu">myandell@genetics.utah.edu</a>; RIVERA, CORBAN GREGORY;
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><a href="mailto:maker-devel@yandell-lab.org">maker-devel@yandell-lab.org</a><o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Subject: Re: [maker-devel] maker2 scripts for functional annotation<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">If it is launching predictors then you have snap hmm or <o:p>
</o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">augustus_species set. You ned to blank out all other options in the
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">control files (including repeat masking options, proteins, ESTs, etc.)
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">when trying to convert mathc/match_part to gene/mRNA/exons/CDS, or else
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">those other programs will run.<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">--Carson<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On 9/25/13 10:31 AM, "<a href="mailto:Xia.Cao@dupont.com">Xia.Cao@dupont.com</a>" <<a href="mailto:Xia.Cao@dupont.com">Xia.Cao@dupont.com</a>> wrote:<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Hi Carson,<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Thank you for the message and your kind help. We tested maker2 by
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">setting keep_preds=1, pred_gff=generated_gff_file_from_first_makerRun .<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">But it seemed maker2 started to launch all predictors again and it
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">took long time to finish. I wonder if there is any way that we can
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">directly get gene/mRNA/exons/CDS gff file without re-running maker2 to
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">convert match/match_part features into gene/mRNA/exons/CDS.<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Thanks,<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Xia<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">-----Original Message-----<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">From: Carson Holt [<a href="mailto:carsonhh@gmail.com">mailto:carsonhh@gmail.com</a>]<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Sent: Thursday, September 19, 2013 5:58 PM<o:p></o:p></p>
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</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">To: Mark Yandell; CAO, XIA; RIVERA, CORBAN GREGORY; <o:p></o:p></p>
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</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><a href="mailto:maker-devel@yandell-lab.org">maker-devel@yandell-lab.org</a><o:p></o:p></p>
</blockquote>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Subject: Re: [maker-devel] maker2 scripts for functional annotation<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
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</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Hello Corban & Xia,<o:p></o:p></p>
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</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
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</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Some scripts like gff3_preds2models are deprecated. To get the same
<o:p></o:p></p>
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</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">result as was offered by gff3_preds2models, just give your <o:p>
</o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">match/match_part features to pref_gff= in the maker_opts.ctl file, set
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">keep_preds=1, and run with all other options and predictors turned off.<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">The final MAKER result will be your match/match_part features
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">converted into gene/mRNA/exons/CDS.<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">For functional annotation, you can use Interproscan, BLASTP against
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">UniProt, or BALST2GO. My preference is to use InterProScan to add GO
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">terms and proteins domains via the ipr_update_gff and iprscan2gff3
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">scripts. Then add putative gene functions via BLASTP to UniProt and
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">maker_functional_fasta and maker_functional_gff scripts.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Go ahead and take a look and that those tools and let me know if you
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">have any questions or need help you configuring them.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Thanks,<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Carson<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">On 9/19/13 11:53 AM, "Mark Yandell" <<a href="mailto:myandell@genetics.utah.edu">myandell@genetics.utah.edu</a>> wrote:<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Hi Corban & Xia,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">I've forwarded your question along to the MAKER_dev list, were you
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">can get speedy answers to your maker related questions. Thanks for
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">using MAKER.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">--mark<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Mark Yandell<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Professor of Human Genetics<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">H.A. & Edna Benning Presidential Endowed Chair Eccles Institute of
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Human Genetics University of Utah<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">15 North 2030 East, Room 2100<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Salt Lake City, UT 84112-5330<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">ph:801-587-7707<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
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<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
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<p class="MsoNormal">________________________________________<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">From: <a href="mailto:Xia.Cao@dupont.com">Xia.Cao@dupont.com</a> [<a href="mailto:Xia.Cao@dupont.com">Xia.Cao@dupont.com</a>]<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Sent: Thursday, September 19, 2013 11:49 AM<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">To: Mark Yandell; <a href="mailto:Corban-Gregory.Rivera@dupont.com">
Corban-Gregory.Rivera@dupont.com</a><o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Subject: maker2 scripts for functional annotation<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Dr. Yandell,<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">We were recently evaluating maker2 for annotation and going through
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">the maker tutorial from 2012.<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><a href="http://gmod.org/wiki/MAKER_Tutorial_2012">http://gmod.org/wiki/MAKER_Tutorial_2012</a><o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">The tutorial makes references to some scripts that we couldnąt find
<o:p></o:p></p>
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</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">in the current release. We were looking for scripts like <o:p>
</o:p></p>
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</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">gff3_preds2models to convert match/match_part format into annotations
<o:p></o:p></p>
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</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">with gene/mRNA/exons/CDS and others. I was wondering if maybe we did
<o:p></o:p></p>
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</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">not have the most up to date version.<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">In addition to getting accurate gene annotations, I was looking for a
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">solution to get functional assignments. I see that there are some
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">scripts like maker_functional_fasta that may help, but I was
<o:p></o:p></p>
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</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">wondering what you would recommend.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Thanks,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Corban & Xia<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">This communication is for use by the intended recipient and contains
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">information that may be Privileged, confidential or copyrighted under
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">applicable law. If you are not the intended recipient, you are hereby
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">formally notified that any use, copying or distribution of this
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">e-mail, in whole or in part, is strictly prohibited. Please notify
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">the sender by return e-mail and delete this e-mail from your system.<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Unless explicitly and conspicuously designated as "E-Contract
<o:p></o:p></p>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Intended", this e-mail does not constitute a contract offer, a
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">contract amendment, or an acceptance of a contract offer. This e-mail
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</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">does not constitute a consent to the use of sender's contact
<o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">information for direct marketing purposes or for transfers of data to
<o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">third parties.<o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">The <a href="http://dupont.com">dupont.com</a> web address will continue in use for a transitional
<o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">period for communications sent or received on behalf of DuPont
<o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Performance Coatings., which is not affiliated in any way with the
<o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">DuPont Company.<o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Francais Deutsch Italiano Espanol Portugues Japanese Chinese
<o:p></o:p></p>
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</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Korean<o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"> <a href="http://www.DuPont.com/corp/email_disclaimer.html">http://www.DuPont.com/corp/email_disclaimer.html</a><o:p></o:p></p>
</blockquote>
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">_______________________________________________<o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">maker-devel mailing list<o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><a href="mailto:maker-devel@box290.bluehost.com">maker-devel@box290.bluehost.com</a><o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><a href="http://box290.bluehost.com/mailman/listinfo/maker-devel_yandell-lab.o">http://box290.bluehost.com/mailman/listinfo/maker-devel_yandell-lab.o</a><o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">r<o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">g<o:p></o:p></p>
</blockquote>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">This communication is for use by the intended recipient and contains
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">information that may be Privileged, confidential or copyrighted under
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">applicable law. If you are not the intended recipient, you are hereby
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">formally notified that any use, copying or distribution of this
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">e-mail, in whole or in part, is strictly prohibited. Please notify the
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">sender by return e-mail and delete this e-mail from your system.
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Unless explicitly and conspicuously designated as "E-Contract
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Intended", this e-mail does not constitute a contract offer, a
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">contract amendment, or an acceptance of a contract offer. This e-mail
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">does not constitute a consent to the use of sender's contact
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">information for direct marketing purposes or for transfers of data to third parties.<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">The <a href="http://dupont.com">dupont.com</a><<a href="http://dupont.com/">http://dupont.com/</a>> web address will continue in use
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">for a transitional period for communications sent or received on
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">behalf of DuPont Performance Coatings., which is not affiliated in any
<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">way with the DuPont Company.<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Francais Deutsch Italiano Espanol Portugues Japanese Chinese
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<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Korean<o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"> <a href="http://www.DuPont.com/corp/email_disclaimer.html">http://www.DuPont.com/corp/email_disclaimer.html</a><o:p></o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">This communication is for use by the intended recipient and contains
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">information that may be Privileged, confidential or copyrighted under
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">applicable law. If you are not the intended recipient, you are hereby
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">formally notified that any use, copying or distribution of this e-mail,
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<p class="MsoNormal">in whole or in part, is strictly prohibited. Please notify the sender
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">by return e-mail and delete this e-mail from your system. Unless
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">explicitly and conspicuously designated as "E-Contract Intended", this
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">e-mail does not constitute a contract offer, a contract amendment, or
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">an acceptance of a contract offer. This e-mail does not constitute a
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">consent to the use of sender's contact information for direct marketing
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">purposes or for transfers of data to third parties.<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">The <a href="http://dupont.com">dupont.com</a><<a href="http://dupont.com/">http://dupont.com/</a>> web address will continue in use for
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">a transitional period for communications sent or received on behalf of
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">DuPont Performance Coatings., which is not affiliated in any way with
<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">the DuPont Company.<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Francais Deutsch Italiano Espanol Portugues Japanese Chinese <o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal">Korean<o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"> <a href="http://www.DuPont.com/corp/email_disclaimer.html">http://www.DuPont.com/corp/email_disclaimer.html</a><o:p></o:p></p>
</blockquote>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal"><o:p> </o:p></p>
</blockquote>
<p class="MsoNormal"><br>
<br>
<br>
This communication is for use by the intended recipient and contains<br>
information that may be Privileged, confidential or copyrighted under<br>
applicable law. If you are not the intended recipient, you are hereby<br>
formally notified that any use, copying or distribution of this e-mail,<br>
in whole or in part, is strictly prohibited. Please notify the sender by<br>
return e-mail and delete this e-mail from your system. Unless explicitly<br>
and conspicuously designated as "E-Contract Intended", this e-mail does<br>
not constitute a contract offer, a contract amendment, or an acceptance<br>
of a contract offer. This e-mail does not constitute a consent to the<br>
use of sender's contact information for direct marketing purposes or for<br>
transfers of data to third parties.<br>
<br>
The <a href="http://dupont.com">dupont.com</a><<a href="http://dupont.com/">http://dupont.com/</a>> web address will continue in use for a<br>
transitional period for communications sent or received on behalf of DuPont<br>
Performance Coatings., which is not affiliated in any way with the DuPont Company.<br>
<br>
Francais Deutsch Italiano Espanol Portugues Japanese Chinese Korean<br>
<br>
<a href="http://www.DuPont.com/corp/email_disclaimer.html">http://www.DuPont.com/corp/email_disclaimer.html</a><br>
<br>
<br>
_______________________________________________<br>
maker-devel mailing list<br>
<a href="mailto:maker-devel@box290.bluehost.com">maker-devel@box290.bluehost.com</a><br>
<a href="http://box290.bluehost.com/mailman/listinfo/maker-devel_yandell-lab.org">http://box290.bluehost.com/mailman/listinfo/maker-devel_yandell-lab.org</a><o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<div>
<div>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black">Barry Moore<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black">Research Scientist<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black">Dept. of Human Genetics<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black">University of Utah<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black">Salt Lake City, UT 84112<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black">--------------------------------------------<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black">(801) 585-3543<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Arial","sans-serif";color:black"><o:p> </o:p></span></p>
</div>
</div>
<div>
<p class="MsoNormal"><span style="font-size:13.5pt;font-family:"Helvetica","sans-serif";color:black"><o:p> </o:p></span></p>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
</div>
<p><br>
This communication is for use by the intended recipient and contains<br>
information that may be Privileged, confidential or copyrighted under<br>
applicable law. If you are not the intended recipient, you are hereby<br>
formally notified that any use, copying or distribution of this e-mail,<br>
in whole or in part, is strictly prohibited. Please notify the sender by<br>
return e-mail and delete this e-mail from your system. Unless explicitly<br>
and conspicuously designated as "E-Contract Intended", this e-mail does<br>
not constitute a contract offer, a contract amendment, or an acceptance<br>
of a contract offer. This e-mail does not constitute a consent to the<br>
use of sender's contact information for direct marketing purposes or for<br>
transfers of data to third parties.</p>
<p>The dupont.com<http://dupont.com/> web address will continue in use for a<br>
transitional period for communications sent or received on behalf of DuPont<br>
Performance Coatings., which is not affiliated in any way with the DuPont Company.</p>
<p>Francais Deutsch Italiano Espanol Portugues Japanese Chinese Korean</p>
<p> http://www.DuPont.com/corp/email_disclaimer.html</p>
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