<div dir="ltr">I have annotated a eukaryotic genome with MAKER 2.30. I recently realized that there are a few genes in the GFF file produced by gff3_merge with inconsistencies in the annotated CDS and UTRs. For most of my genes, the UTRs have their own lines in the GFF file. However, for the problematic genes, the UTRs are not specified in the GFF file and all exons are annotated as CDS. The UTRs do appear in the gene header and the protein sequences are the correct length (do not include the UTR). I have attached an example from the GFF file. <div>
<br></div><div>Is this a known problem, or have I done something wrong? Is there an easy way to fix the GFF file? </div><div><br></div><div>Thanks for your help,</div><div>Ben<br clear="all"><div><br></div>-- <br><div dir="ltr">
<span style="font-family:arial,sans-serif;font-size:13px;border-collapse:collapse">_____________________________________________________<br>Benjamin ER Rubin</span><div><span style="font-family:arial,sans-serif;font-size:13px;border-collapse:collapse">PhD Candidate</span><div>
<span style="font-family:arial,sans-serif;font-size:13px;border-collapse:collapse"><span style="border-collapse:separate;font-family:arial;font-size:small"><div><span style="font-family:arial,sans-serif;font-size:13px;border-collapse:collapse">Committee on Evolutionary Biology</span></div>
<div><span style="font-family:arial,sans-serif;font-size:13px;border-collapse:collapse">University of Chicago</span></div><div><span style="font-family:arial,sans-serif;font-size:13px;border-collapse:collapse"><a href="http://benrubin.org" target="_blank"><font color="#0658b5">b</font>enrubin.org</a></span></div>
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