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<p class="MsoNormal">Hi, I am trying to use the maker_functional_gff program to add functional annotations to my maker gff file. I used blastp with the tabular “-outfmt 6” option against the uniprot uniref-50. I put these results in the maker_functional_gff
program using “maker_functional_gff uniref-50 blastp-output maker.gff” but I get the following errors and no updating of the names in my maker gff file:
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Use of uninitialized value $id in hash element at /home/b/maker/bin/maker_functional_gff line 142, <$IN> line 16924097.<o:p></o:p></p>
<p class="MsoNormal">Use of uninitialized value $id in hash element at /home/b/maker/bin/maker_functional_gff line 144, <$IN> line 16924097.<o:p></o:p></p>
<p class="MsoNormal">Can't parse details from FASTA header: >UniRef50_K1R9E3 Uncharacterized protein n=1 Tax=Crassostrea gigas RepID=K1R9E3_CRAGI<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Use of uninitialized value $id in hash element at /home/b/maker/bin/maker_functional_gff line 142, <$IN> line 16924128.<o:p></o:p></p>
<p class="MsoNormal">Use of uninitialized value $id in hash element at /home/b/maker/bin/maker_functional_gff line 144, <$IN> line 16924128.<o:p></o:p></p>
<p class="MsoNormal">Can't parse details from FASTA header: >UniRef50_K1R9E4 Transporter n=2 Tax=Mollusca RepID=K1R9E4_CRAGI<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Any ideas of what I’m doing wrong?<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Brian<o:p></o:p></p>
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