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<p class="MsoNormal">Hi, I thought I would try asking my question here as NCBI was not able to give me much assistance. In preparation for submitting to NCBI, I converted my my MAKER gff3 to NCBI tbl format using the gff32tbl script that Carson posted a link
to in this thread (<a href="http://gmod.827538.n3.nabble.com/NCBI-feature-table-tt4040473.html#a4040475">http://gmod.827538.n3.nabble.com/NCBI-feature-table-tt4040473.html#a4040475</a>). It seemed to have converted fine, however when I use NCBIs tbl2asn program
I get numerous errors in my errorsummary.val file:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"> 4 ERROR: SEQ_FEAT.BadTrailingCharacter<o:p></o:p></p>
<p class="MsoNormal"> 217 ERROR: SEQ_FEAT.NoStop<o:p></o:p></p>
<p class="MsoNormal"> 438 ERROR: SEQ_FEAT.ShortIntron<o:p></o:p></p>
<p class="MsoNormal"> 171 ERROR: SEQ_FEAT.StartCodon<o:p></o:p></p>
<p class="MsoNormal"> 171 ERROR: SEQ_INST.BadProteinStart<o:p></o:p></p>
<p class="MsoNormal"> 291 WARNING: SEQ_FEAT.NotSpliceConsensusAcceptor<o:p></o:p></p>
<p class="MsoNormal"> 648 WARNING: SEQ_FEAT.NotSpliceConsensusDonor<o:p></o:p></p>
<p class="MsoNormal"> 118 WARNING: SEQ_FEAT.ShortExon<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">In addition, all of the genes, cds, and mRNA coordinates in the resulting sqn files are decreased by one. For example my tbl file will have gene coordinates of 440869 – 441931, but the sqn file will have 440868 – 441930. Any ideas what
might be causing this?<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks,<o:p></o:p></p>
<p class="MsoNormal">Brian<o:p></o:p></p>
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