<div dir="ltr"><div><div>Hi Carson,<br><br></div>I am using MAKER to annotate a few small genomes. When looking through the gff file, I notice that the exon numbers do not start from 0 or 1 for each gene. Only the first gene in a scaffold start with exon 0. If the first gene has 3 exons (0-2), then the second gene will start from exon 3 (an example is shown below). It seems many people would prefer that in each gene, the first exon be exon 1. Is it possible to make such a change?  Thanks.<br>
<br></div>Guohong<br><div><br><br>scaffold1       .       contig  1       347483  .       .       .       ID=scaffold1;Name=scaffold1<br>scaffold1       maker   gene    106     2985    .       +       .       ID=genemark-scaffold1-processed-gene-0.0;Name=genemark-scaffold1-processed-gene-0.0<br>
scaffold1       maker   mRNA    106     2985    .       +       .       ID=genemark-scaffold1-processed-gene-0.0-mRNA-1;Parent=genemark-scaffold1-processed-gene-0.0;Name=genemark-scaffold1-processed-gene-0.0-mRNA-1;_AED=0.12;_eAED=0.13;_QI=0|0|0|1|1|1|3|0|840<br>
scaffold1       maker   exon    106     1684    .       +       .       ID=genemark-scaffold1-processed-gene-0.0-mRNA-1:exon:0;Parent=genemark-scaffold1-processed-gene-0.0-mRNA-1<br>scaffold1       maker   exon    1878    2440    .       +       .       ID=genemark-scaffold1-processed-gene-0.0-mRNA-1:exon:1;Parent=genemark-scaffold1-processed-gene-0.0-mRNA-1<br>
scaffold1       maker   exon    2605    2985    .       +       .       ID=genemark-scaffold1-processed-gene-0.0-mRNA-1:exon:2;Parent=genemark-scaffold1-processed-gene-0.0-mRNA-1<br>scaffold1       maker   CDS     106     1684    .       +       0       ID=genemark-scaffold1-processed-gene-0.0-mRNA-1:cds;Parent=genemark-scaffold1-processed-gene-0.0-mRNA-1<br>
scaffold1       maker   CDS     1878    2440    .       +       2       ID=genemark-scaffold1-processed-gene-0.0-mRNA-1:cds;Parent=genemark-scaffold1-processed-gene-0.0-mRNA-1<br>scaffold1       maker   CDS     2605    2985    .       +       0       ID=genemark-scaffold1-processed-gene-0.0-mRNA-1:cds;Parent=genemark-scaffold1-processed-gene-0.0-mRNA-1<br>
scaffold1       maker   gene    38466   41604   .       +       .       ID=maker-scaffold1-snap-gene-0.254;Name=maker-scaffold1-snap-gene-0.254<br>scaffold1       maker   mRNA    38466   41604   .       +       .       ID=maker-scaffold1-snap-gene-0.254-mRNA-1;Parent=maker-scaffold1-snap-gene-0.254;Name=maker-scaffold1-snap-gene-0.254-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|0.83|1|1|6|0|892<br>
scaffold1       maker   exon    38466   38511   .       +       .       ID=maker-scaffold1-snap-gene-0.254-mRNA-1:exon:3;Parent=maker-scaffold1-snap-gene-0.254-mRNA-1<br>scaffold1       maker   exon    38616   38742   .       +       .       ID=maker-scaffold1-snap-gene-0.254-mRNA-1:exon:4;Parent=maker-scaffold1-snap-gene-0.254-mRNA-1<br>
scaffold1       maker   exon    38831   39986   .       +       .       ID=maker-scaffold1-snap-gene-0.254-mRNA-1:exon:5;Parent=maker-scaffold1-snap-gene-0.254-mRNA-1<br>scaffold1       maker   exon    40073   40154   .       +       .       ID=maker-scaffold1-snap-gene-0.254-mRNA-1:exon:6;Parent=maker-scaffold1-snap-gene-0.254-mRNA-1<br>
scaffold1       maker   exon    40259   40666   .       +       .       ID=maker-scaffold1-snap-gene-0.254-mRNA-1:exon:7;Parent=maker-scaffold1-snap-gene-0.254-mRNA-1<br>scaffold1       maker   exon    40745   41604   .       +       .       ID=maker-scaffold1-snap-gene-0.254-mRNA-1:exon:8;Parent=maker-scaffold1-snap-gene-0.254-mRNA-1<br>
scaffold1       maker   CDS     38466   38511   .       +       0       ID=maker-scaffold1-snap-gene-0.254-mRNA-1:cds;Parent=maker-scaffold1-snap-gene-0.254-mRNA-1<br>scaffold1       maker   CDS     38616   38742   .       +       2       ID=maker-scaffold1-snap-gene-0.254-mRNA-1:cds;Parent=maker-scaffold1-snap-gene-0.254-mRNA-1<br>
scaffold1       maker   CDS     38831   39986   .       +       1       ID=maker-scaffold1-snap-gene-0.254-mRNA-1:cds;Parent=maker-scaffold1-snap-gene-0.254-mRNA-1<br>scaffold1       maker   CDS     40073   40154   .       +       0       ID=maker-scaffold1-snap-gene-0.254-mRNA-1:cds;Parent=maker-scaffold1-snap-gene-0.254-mRNA-1<br>
scaffold1       maker   CDS     40259   40666   .       +       2       ID=maker-scaffold1-snap-gene-0.254-mRNA-1:cds;Parent=maker-scaffold1-snap-gene-0.254-mRNA-1<br>scaffold1       maker   CDS     40745   41604   .       +       2       ID=maker-scaffold1-snap-gene-0.254-mRNA-1:cds;Parent=maker-scaffold1-snap-gene-0.254-mRNA-1<br>
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