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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;">Hi Panos,
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<div>1) You'll only use est2genome and protein2genome for creating models that will be used for training the ab-initio predictors (like SNAP). Sometimes that means one run of MAKER for training; sometimes that means two runs of MAKER. You usually don't gain
any accuracy after the second round of training. It's ok to use both EST and protein data for this training step. </div>
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<div>2) If you're using both ESTs and protein sequence to train your ab-initio predictors, then both est2genome and protein2genome should be set to 1. </div>
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<div>3) If you want to pass Blast results to MAKER, you'll need to pass those results as GFF3, but MAKER will install and run blast for you, and does a good job of keeping track of all those results and making them accessible to you in the end, so it's going
to be a lot of work to do those blasts on your own outside of MAKER. I seriously suggest that you use blast internal to maker. <br>
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<div class="PlainText">Daniel Ence<br>
Graduate Student<br>
Eccles Institute of Human Genetics<br>
University of Utah<br>
15 North 2030 East, Room 2100<br>
Salt Lake City, UT 84112-5330</div>
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<div id="divRpF103033" style="direction: ltr;"><font face="Tahoma" size="2" color="#000000"><b>From:</b> maker-devel [maker-devel-bounces@yandell-lab.org] on behalf of Panos Ioannidis [panos.ioannidis@gmail.com]<br>
<b>Sent:</b> Friday, July 11, 2014 5:56 AM<br>
<b>To:</b> maker-devel<br>
<b>Subject:</b> [maker-devel] (no subject)<br>
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<div dir="ltr">I got back to my annotations this past week and have a couple of questions!
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<div>1) Since my organism isn't closely related with any other that's already sequenced, I will have to run maker twice (according to the tutorial). So for the first run I see that some people use only the ESTs and some others use ESTs and a protein database
(CEGMA, Uniref50, Swiss-Prot, etc). I guess that the ESTs will give better models, but for the cases where genes aren't covered by an EST, it's okay to have a protein database to detect them as well. Am I right? What do you think?</div>
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<div>2) In case I use both ESTs and a protein database how should I set the <font face="courier new, monospace">
est2genome</font> and <font face="courier new, monospace">protein2genome</font> parameters in the
<font face="courier new, monospace">maker_opts.ctl</font> file? Should they both equal to "1"?</div>
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<div>3) I've been thinking of running the BLAST searches separately and giving Maker directly the results. I guess that in this case, I'll have to first convert the BLAST output to a gff3 file and give it to the
<font face="courier new, monospace">protein_gff</font> parameter, right?</div>
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<div>Thanks,</div>
<div>Panos</div>
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