<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; color: rgb(0, 0, 0); font-size: 14px; font-family: Calibri, sans-serif;"><div>If it happening only with GFF3 pass-through, then it may be something I saw and fixed a while ago (there were some GFF3 passthrough fixes since 2.31.4). Could you check and see if it still happens in 2.31.6. Also if it is only the first or last CDS/exon, then Augustus can do that and it's not actually a bug. Basically it is truncating the model to the start/stop codon so the first or last exon/CDS may appear short, but it's really just incomplete. If you can find any example of a non-CDS/exon feature then could you send it to me?</div><div><br></div><div>Thanks,</div><div>Carson</div><div><br></div><div><br></div><span id="OLK_SRC_BODY_SECTION"><div style="font-family:Calibri; font-size:11pt; text-align:left; color:black; BORDER-BOTTOM: medium none; BORDER-LEFT: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 0in; PADDING-RIGHT: 0in; BORDER-TOP: #b5c4df 1pt solid; BORDER-RIGHT: medium none; PADDING-TOP: 3pt"><span style="font-weight:bold">From: </span> Marc Höppner <<a href="mailto:mphoeppner@gmail.com">mphoeppner@gmail.com</a>><br><span style="font-weight:bold">Date: </span> Wednesday, July 30, 2014 at 4:44 AM<br><span style="font-weight:bold">To: </span> <<a href="mailto:maker-devel@yandell-lab.org">maker-devel@yandell-lab.org</a>><br><span style="font-weight:bold">Subject: </span> [maker-devel] Maker GFF output with features of 0 length<br></div><div><br></div><div><meta http-equiv="Content-Type" content="text/html charset=windows-1252"><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Hi,<div><br></div><div>I’ve - more by accident - found that many of the gene builds I have generated with Maker (2.31.3) contain features with identical start and stop positions. </div><div><br></div><div>For example:</div><div><br></div><div><div style="margin: 0px; font-family: Menlo;">scaffold_2927 maker CDS 13013 13013 . + 1 ID=maker-scaffold_2927-augustus-gene-0.8-mRNA-1:cds;Parent=maker-scaffold_2927-augustus-gene-0.8-mRNA-1</div></div><div><br></div><div><br></div><div>This occurs seemingly randomly for all sorts of feature types and I have only seen this when running Maker on full assemblies. Before I start turning every stone, any ideas about possible explanations for this phenomenon? Is this likely some MPI-related communication issue, or NFS problems with synching data? Maker runs fine on our system, but that doesn’t mean that there aren’t any cryptic issues that only on these occasions read their head… Regarding the frequency, out of 450.000 GFF lines, 270 were affected in the case that I looked into the most. So it is pretty rare, but still...</div><div><br></div><div>I am currently using Maker with openmpi-1.7.4 and the file system is mounter of NFS4 and IPoIB. I now switched to Maker 2.31.6, but have no strong reason to suspect that this will make a difference.</div><div><br></div><div>Regards,</div><div><br></div><div>Marc</div><div><br></div><div><br></div></div></div>_______________________________________________
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