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<span id="mailbox-conversation"><div>Hi Carson and Jason,</div>
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<div>I’ve heard you guys say several times on this list serve that Trinity is the preferable option for ESTs (vs. Cufflinks). Does this mean the genome-guided or the strict denovo approach?</div>
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<div>Second, Jason has mentioned using PASA for generating a “best set of annotations” for use in Augustus training. I have seen his post (and repost) of this, however there aren’t many details on how to use PASA to accomplish this. Any chance that you could post a more detailed walkthrough on how to approach this?</div>
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<div>Best,</div>
<div>Jason Gallant</div></span><div class="mailbox_signature">
<br>—<br>Dr. Jason R. Gallant<div>Assistant Professor</div>
<div>Room 38 Natural Sciences<br><div>Department of Zoology</div>
<div>Michigan State University</div>
<div>East Lansing, MI 48824</div>
<div>jgallant@msu.edu</div>
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<div>office: 517-884-7756</div>
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