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<DIV>
<DIV><FONT face="Times New Roman">Hello, </FONT></DIV>
<DIV><FONT face="Times New Roman"></FONT> </DIV>
<DIV><FONT
face="Times New Roman">When I use the maker for geomic annotation pipeline, I met a very big problem. My genome has 7282 scaffolds, all is success but for one scaffold is failed. I donot konw why and I have check the log, but I still can't fix the problem. So I need you help, and I will appreciate it very much. </FONT></DIV>
<DIV><FONT face="Times New Roman"></FONT> </DIV>
<DIV><FONT
face="Times New Roman">Here is a part of main error log: </FONT></DIV>
<BLOCKQUOTE style="MARGIN-RIGHT: 0px" dir=ltr>
<DIV><FONT
face="Times New Roman">WARNING: Cannot find >0, trying to re-index the fasta. </FONT></DIV>
<DIV><FONT face="Times New Roman">stop here:0 </FONT></DIV>
<DIV><FONT
face="Times New Roman">ERROR: Fasta index error </FONT></DIV>
<DIV><FONT
face="Times New Roman"> at /disk/yinchuanlin/software/maker/maker/bin/../lib/GI.pm line 1622. </FONT></DIV>
<DIV><FONT
face="Times New Roman"> GI::polish_exonerate(FastaChunk=HASH(0x7fe0263563b8), FastaSeq=HASH(0x3f7b280), 2988503, ">contig1", ARRAY(0x3d3ad80), ARRAY(0x3d17ed0), "/disk/yinchuanlin/01_pingguodue_project/02_OMIGA/09_maker_wor"..., "e", "/disk/yinchuanlin/software/maker/exonerate-2.2.0-x86_64/bin/e"..., ...) called at /disk/yinchuanlin/software/maker/maker/bin/../lib/Process/MpiChunk.pm line 1986 </FONT></DIV>
<DIV><FONT
face="Times New Roman"> Process::MpiChunk::__ANON__() called at /disk/yinchuanlin/software/maker/maker/bin/../lib/Error.pm line 415 </FONT></DIV>
<DIV><FONT
face="Times New Roman"> eval {...} called at /disk/yinchuanlin/software/maker/maker/bin/../lib/Error.pm line 407 </FONT></DIV>
<DIV><FONT
face="Times New Roman"> Error::subs::try(CODE(0x3f381b8), HASH(0x3f62380)) called at /disk/yinchuanlin/software/maker/maker/bin/../lib/Process/MpiChunk.pm line 4224 </FONT></DIV>
<DIV><FONT
face="Times New Roman"> Process::MpiChunk::_go(Process::MpiChunk=HASH(0x3e30020), "run", HASH(0x7fe026352410), 2, 3) called at /disk/yinchuanlin/software/maker/maker/bin/../lib/Process/MpiChunk.pm line 341 </FONT></DIV>
<DIV><FONT
face="Times New Roman"> Process::MpiChunk::run(Process::MpiChunk=HASH(0x3e30020), 0) called at /disk/yinchuanlin/software/maker/maker/bin/../lib/Process/MpiChunk.pm line 357 </FONT></DIV>
<DIV><FONT
face="Times New Roman"> Process::MpiChunk::run_all(Process::MpiChunk=HASH(0x3e30020), 0) called at /disk/yinchuanlin/software/maker/maker/bin/../lib/Process/MpiTiers.pm line 287 </FONT></DIV>
<DIV><FONT
face="Times New Roman"> Process::MpiTiers::run_all(Process::MpiTiers=HASH(0x3d43310), 0) called at /disk/yinchuanlin/software/maker/maker/bin/../lib/Process/MpiTiers.pm line 287 </FONT></DIV>
<DIV><FONT
face="Times New Roman"> Process::MpiTiers::run_all(Process::MpiTiers=HASH(0x3f28b28), 0) called at /disk/yinchuanlin/software/maker/maker/bin/maker line 686 </FONT></DIV>
<DIV><FONT
face="Times New Roman">--> rank=NA, hostname=node7 </FONT></DIV>
<DIV><FONT
face="Times New Roman">ERROR: Failed while polishig ESTs </FONT></DIV>
<DIV><FONT
face="Times New Roman">ERROR: Chunk failed at level:2, tier_type:3 </FONT></DIV>
<DIV><FONT
face="Times New Roman">FAILED CONTIG:contig1 </FONT></DIV>
<DIV><FONT face="Times New Roman"></FONT> </DIV>
<DIV><FONT
face="Times New Roman">ERROR: Chunk failed at level:4, tier_type:0 </FONT></DIV>
<DIV><FONT
face="Times New Roman">FAILED CONTIG:contig1 </FONT></DIV></BLOCKQUOTE>
<DIV><FONT face="Times New Roman"></FONT> </DIV>
<DIV><FONT face="Times New Roman">Thanks! </FONT></DIV>
<DIV><FONT face="Times New Roman"></FONT> </DIV>
<DIV><FONT face="Times New Roman">Best regards, </FONT></DIV>
<DIV><FONT face="Times New Roman">Ian </FONT></DIV>
<DIV><FONT
face="Times New Roman">Department of Entomology, College of Plant Protection, Nanjing Agricultural University </FONT></DIV>
<DIV><FONT
face="Times New Roman">No. 1, Weigang Road, Xuanwu District, Nanjing, Jiangsu 210095, China </FONT></DIV>
<DIV><FONT face="Times New Roman"></FONT> </DIV>
<DIV><FONT
face="Times New Roman"></FONT> </DIV></DIV></STATIONERY></BODY></HTML>