<div dir="ltr"><span style="font-size:12.8px">Hi all,</span><div style="font-size:12.8px"><br></div><div style="font-size:12.8px">The following errors occurred while I was using MAKER for annotation. I have searched around this forum but seems like the solutions provided do not works for me.</div><div style="font-size:12.8px"><br></div><div style="font-size:12.8px">#################################################################</div><div style="font-size:12.8px"><br></div><div style="font-size:12.8px"><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">------------- EXCEPTION: Bio::Root::Exception -------------</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">MSG: Must have defined a valid name for Hit</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Error::throw</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Bio::Root::Root::throw /usr/local/share/perl5/Bio/Root/Root.pm:486</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Bio::Search::Hit::GenericHit::new /usr/local/share/perl5/Bio/Search/Hit/GenericHit.pm:149</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Bio::Search::Hit::PhatHit::Base::new maker/bin/../lib/Bio/Search/Hit/PhatHit/Base.pm:127</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Bio::Search::Hit::PhatHit::blastx::new maker/bin/../lib/Bio/Search/Hit/PhatHit/<a href="http://blastx.pm:125/" target="_blank">blastx.pm:125</a></span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Bio::Search::Hit::HitFactory::create /usr/local/share/perl5/Bio/Search/Hit/HitFactory.pm:124</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Bio::Factory::ObjectFactoryI::create_object /usr/local/share/perl5/Bio/Factory/ObjectFactoryI.pm:114</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Bio::Search::Iteration::GenericIteration::newhits_below_threshold /usr/local/share/perl5/Bio/Search/Iteration/GenericIteration.pm:506</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Bio::Search::Iteration::GenericIteration::newhits /usr/local/share/perl5/Bio/Search/Iteration/GenericIteration.pm:488</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Bio::Search::Iteration::GenericIteration::hits /usr/local/share/perl5/Bio/Search/Iteration/GenericIteration.pm:469</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Bio::Search::Result::BlastResult::hits /usr/local/share/perl5/Bio/Search/Result/BlastResult.pm:168</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Bio::Search::Result::BlastResult::num_hits /usr/local/share/perl5/Bio/Search/Result/BlastResult.pm:242</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Widget::blastx::keepers</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">maker/bin/../lib/Widget/<a href="http://blastx.pm:164/" target="_blank">blastx.pm:164</a></span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Widget::blastx::parse</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">maker/bin/../lib/Widget/<a href="http://blastx.pm:132/" target="_blank">blastx.pm:132</a></span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: GI::blastx_as_chunks</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">maker/bin/../lib/GI.pm:2457</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: GI::blastx_as_chunks</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">maker/bin/../lib/GI.pm:2466</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Process::MpiChunk::_go</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">maker/bin/../lib/Process/MpiChunk.pm:2687</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Process::MpiChunk::run</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">maker/bin/../lib/Process/MpiChunk.pm:341</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Process::MpiChunk::run_all</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">maker/bin/../lib/Process/MpiChunk.pm:357</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Process::MpiTiers::run_all maker/bin/../lib/Process/MpiTiers.pm:287</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: Process::MpiTiers::run_all maker/bin/../lib/Process/MpiTiers.pm:287</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">STACK: maker/bin/maker:686</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">-----------------------------------------------------------</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">--> rank=NA, hostname=gena2</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">--> rank=NA, hostname=gena2</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">--> rank=NA, hostname=gena2</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">--> rank=NA, hostname=gena2</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">ERROR: Failed while doing blastx of proteins</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">ERROR: Chunk failed at level:8, tier_type:3</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">FAILED CONTIG:Contig1</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'"> </span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">ERROR: Chunk failed at level:4, tier_type:0</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">FAILED CONTIG:Contig1</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'"> </span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">examining contents of the fasta file and run log</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'"> </span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'">###########################################################</span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><span style="font-family:'Courier New'"><br></span></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><font face="Courier New">Is anyone has any idea on this? </font></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><font face="Courier New"><br></font></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><font face="Courier New">Thanks,</font></p><p class="MsoNormal" style="margin-bottom:0.0001pt"><font face="Courier New">SokLim</font></p></div></div>