<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Make sure your using the current version of MAKER. That same thread mentions it was fixed once they updated from 2.31.3<div class=""><br class=""></div><div class="">Current version is 2.31.8.</div><div class=""><br class=""></div><div class="">—Carson</div><div class=""><br class=""><div class=""><br class=""></div><div class=""><br class=""><div><blockquote type="cite" class=""><div class="">On May 26, 2016, at 1:54 PM, Jeremy DeBarry <<a href="mailto:debarryj@gmail.com" class="">debarryj@gmail.com</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div dir="ltr" class="">Greetings,<div class=""> My group has run MAKER on a small genome. One of the annotated tRNAs has an intron. The two exons are annotated on different strands. The gene and first exon are on the + strand and the second exon is on the - strand.</div><div class=""><br class=""></div><div class=""> I looked over the archives and found <a href="https://groups.google.com/forum/#!searchin/maker-devel/trnascan$20strand/maker-devel/n09EMqCP_Do/08AJuaZ1K9QJ" class="">previous reports</a>, but it appears they apply to earlier versions of MAKER.</div><div class=""><br class=""></div><div class=""> My instinct is to 'manually' correct the strand information for the - strand exon, but I wanted to investigate the issue further first.</div><div class=""><br class=""></div><div class=""> Do you have any insight?</div><div class=""><br class=""></div><div class="">Much appreciated,</div><div class="">Jeremy<br clear="all" class=""><div class=""><div class="gmail_signature"><div dir="ltr" class=""><div class=""><div dir="ltr" class=""><div dir="ltr" class=""><div dir="ltr" class=""><div dir="ltr" class=""><div dir="ltr" class=""><div dir="ltr" class=""><div dir="ltr" class=""><div class=""><br class=""></div><div class="">--<br class=""></div><a href="http://orcid.org/0000-0001-5718-2675" target="_blank" class="">Dr. Jeremy DeBarry PhD</a></div><div class=""><span style="color:rgb(0,0,238);font-size:12.8px;text-decoration:underline" class=""><a href="http://www.systemsbiology.emory.edu/" target="_blank" class="">MaHPIC Data Coordinator</a></span></div><div class=""><a href="http://mango.ctegd.uga.edu/jkissingLab/" target="_blank" class="">Kissinger Research Group<br class=""></a></div><div dir="ltr" class=""><a href="http://www.uga.edu/" target="_blank" class="">The University of Georgia</a><br class=""><div class=""><div class="">:::</div><div class="">Email: <a href="mailto:debarryj@gmail.com" target="_blank" class="">debarryj@gmail.com</a></div><div style="font-size:12.8px" class="">Tel: +1.912.269.0484</div><div style="font-size:12.8px" class=""></div><div class="">Skype ID: jdebarry</div><div class=""><span style="font-size:12.8px" class="">:::</span><br class=""></div><div class=""><div class="">Nihil Sine Labore!:::Nec Aspera Terrent!:::Boutez-en-Avant!</div></div></div></div></div></div></div></div></div></div></div></div></div></div>
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