<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
<style type="text/css" style="display:none"><!--P{margin-top:0;margin-bottom:0;} --></style>
</head>
<body dir="ltr" style="font-size:12pt;color:#000000;background-color:#FFFFFF;font-family:Calibri,Arial,Helvetica,sans-serif;">
<p>Dear Maker, </p>
<p><br>
</p>
<p>I did run maker for the annotation of a <em>denovo</em> genome assembly. It works great for all scaffolds but 1.
</p>
<p><br>
</p>
<p>Here is the error I get:</p>
<p><br>
</p>
<p>WARNING: Cannot find >0, trying to re-index the fasta.<br>
stop here:0<br>
ERROR: Fasta index error<br>
at /scratch/beegfs/monthly/vrossier/SOFT/MAKER2/maker_mpich/bin/../lib/GI.pm line 1622.<br>
GI::polish_exonerate('FastaChunk=HASH(0x5fd22b8)', 'FastaSeq=HASH(0x4607540)', 211829, '>scaffold52256_cov106', 'ARRAY(0x62a2240)', 'ARRAY(0x629f198)', '/scratch/beegfs/monthly/vrossier/temp/aoc_denovo_maker/Aocell...', 'a', '/software/SequenceAnalysis/SequenceAlignment/exonerate/2.2.0/...',
...) called at /scratch/beegfs/monthly/vrossier/SOFT/MAKER2/maker_mpich/bin/../lib/Process/MpiChunk.pm line 2491<br>
Process::MpiChunk::__ANON__() called at /scratch/beegfs/monthly/vrossier/SOFT/MAKER2/maker_mpich/bin/../lib/Error.pm line 415<br>
eval {...} called at /scratch/beegfs/monthly/vrossier/SOFT/MAKER2/maker_mpich/bin/../lib/Error.pm line 407<br>
Error::subs::try('CODE(0x45d40a8)', 'HASH(0x5fe4930)') called at /scratch/beegfs/monthly/vrossier/SOFT/MAKER2/maker_mpich/bin/../lib/Process/MpiChunk.pm line 4224<br>
Process::MpiChunk::_go('Process::MpiChunk=HASH(0x45ddfb0)', 'run', 'HASH(0x4e3c540)', 6, 3) called at /scratch/beegfs/monthly/vrossier/SOFT/MAKER2/maker_mpich/bin/../lib/Process/MpiChunk.pm line 341<br>
Process::MpiChunk::run('Process::MpiChunk=HASH(0x45ddfb0)', 16) called at /scratch/beegfs/monthly/vrossier/SOFT/MAKER2/maker_mpich/bin/maker line 979<br>
--> rank=16, hostname=dee-serv04.vital-it.ch<br>
ERROR: Failed while polishing alt-ESTs<br>
ERROR: Chunk failed at level:6, tier_type:3<br>
FAILED CONTIG:scaffold52256_cov106<br>
<br>
ERROR: Chunk failed at level:4, tier_type:0<br>
FAILED CONTIG:scaffold52256_cov106</p>
<p><br>
</p>
<p>Any thought how to solve this?</p>
<p><br>
</p>
<p>Thanks in advance,</p>
<p>Victor Rossier <br>
</p>
</body>
</html>