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<DIV><FONT face="Times New Roman">Hello, </FONT></DIV>
<DIV><FONT face="Times New Roman"></FONT> </DIV>
<DIV><FONT
face="Times New Roman">When I use the maker for geomic annotation pipeline, I met a very big problem. My genome has 7282 scaffolds, all is success but for one scaffold is failed. I don‘t konw why and I have check the log, but I still can't fix the problem. So I need you help, and I will appreciate it very much. </FONT></DIV>
<DIV><FONT face="Times New Roman"></FONT> </DIV>
<DIV><FONT
face="Times New Roman">Here is a part of main error log: </FONT></DIV>
<BLOCKQUOTE style="MARGIN-RIGHT: 0px" dir=ltr>
<DIV><FONT face="Times New Roman">------------- EXCEPTION:
Bio::Root::Exception -------------<BR>MSG: Can't get HSPs: data not
collected.<BR>STACK: Error::throw<BR>STACK: Bio::Root::Root::throw
/usr/local/share/perl5/Bio/Root/Root.pm:449<BR>STACK:
Bio::Search::Hit::PhatHit::Base::hsps
/disk/home/yincl/software/maker/maker/bin/../lib/Bio/Search/Hit/PhatHit/Base.pm:552<BR>STACK:
Widget::blastx::keepers
/disk/home/yincl/software/maker/maker/bin/../lib/Widget/blastx.pm:190<BR>STACK:
Widget::blastx::parse
/disk/home/yincl/software/maker/maker/bin/../lib/Widget/blastx.pm:132<BR>STACK:
GI::blastx
/disk/home/yincl/software/maker/maker/bin/../lib/GI.pm:2622<BR>STACK:
GI::blastx
/disk/home/yincl/software/maker/maker/bin/../lib/GI.pm:2631<BR>STACK:
GI::reblast_merged_hits
/disk/home/yincl/software/maker/maker/bin/../lib/GI.pm:445<BR>STACK:
GI::merge_resolve_hits
/disk/home/yincl/software/maker/maker/bin/../lib/GI.pm:289<BR>STACK:
Process::MpiChunk::_go
/disk/home/yincl/software/maker/maker/bin/../lib/Process/MpiChunk.pm:2821<BR>STACK:
Process::MpiChunk::run
/disk/home/yincl/software/maker/maker/bin/../lib/Process/MpiChunk.pm:341<BR>STACK:
Process::MpiChunk::run_all
/disk/home/yincl/software/maker/maker/bin/../lib/Process/MpiChunk.pm:357<BR>STACK:
Process::MpiTiers::run_all
/disk/home/yincl/software/maker/maker/bin/../lib/Process/MpiTiers.pm:287<BR>STACK:
Process::MpiTiers::run_all
/disk/home/yincl/software/maker/maker/bin/../lib/Process/MpiTiers.pm:287<BR>STACK:
/disk/home/yincl/software/maker/maker/bin/maker:686<BR>-----------------------------------------------------------<BR>-->
rank=NA, hostname=localhost.localdomain<BR>--> rank=NA,
hostname=localhost.localdomain<BR>--> rank=NA,
hostname=localhost.localdomain<BR>ERROR: Failed while collecting blastx
reports<BR>ERROR: Chunk failed at level:9, tier_type:3<BR>FAILED
CONTIG:QPacbio.Hiseq_683</FONT></DIV>
<DIV> </DIV>
<DIV><FONT face="Times New Roman">ERROR: Chunk failed at level:4,
tier_type:0<BR>FAILED CONTIG:QPacbio.Hiseq_683</FONT></DIV>
<DIV> </DIV>
<DIV><FONT face="Times New Roman">examining contents of the fasta file and run
log</FONT></DIV></BLOCKQUOTE>
<DIV><FONT face="Times New Roman">Thanks! </FONT></DIV>
<DIV><FONT face="Times New Roman"></FONT> </DIV>
<DIV><FONT face="Times New Roman">Best regards, </FONT></DIV>
<DIV><FONT face="Times New Roman">Ian </FONT></DIV>
<DIV><FONT
face="Times New Roman">Department of Entomology, College of Plant Protection, Nanjing Agricultural University </FONT></DIV>
<DIV><FONT
face="Times New Roman">No. 1, Weigang Road, Xuanwu District, Nanjing, Jiangsu 210095, China </FONT></DIV>
<DIV><FONT face="Times New Roman"></FONT> </DIV>
<DIV><FONT
face="Times New Roman"></FONT> </DIV></DIV></STATIONERY></BODY></HTML>