<html>
<head>
<meta content="text/html; charset=utf-8" http-equiv="Content-Type">
</head>
<body bgcolor="#FFFFFF" text="#000000">
<p>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
</p>
<p>
<meta name="ProgId" content="Word.Document">
<meta name="Generator" content="Microsoft Word 12">
<meta name="Originator" content="Microsoft Word 12">
<link rel="File-List"
href="file:///C:%5CUsers%5Camine%5CAppData%5CLocal%5CTemp%5Cmsohtmlclip1%5C01%5Cclip_filelist.xml">
<link rel="themeData"
href="file:///C:%5CUsers%5Camine%5CAppData%5CLocal%5CTemp%5Cmsohtmlclip1%5C01%5Cclip_themedata.thmx">
<link rel="colorSchemeMapping"
href="file:///C:%5CUsers%5Camine%5CAppData%5CLocal%5CTemp%5Cmsohtmlclip1%5C01%5Cclip_colorschememapping.xml">
<!--[if gte mso 9]><xml>
<w:WordDocument>
<w:View>Normal</w:View>
<w:Zoom>0</w:Zoom>
<w:TrackMoves/>
<w:TrackFormatting/>
<w:HyphenationZone>21</w:HyphenationZone>
<w:PunctuationKerning/>
<w:ValidateAgainstSchemas/>
<w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid>
<w:IgnoreMixedContent>false</w:IgnoreMixedContent>
<w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText>
<w:DoNotPromoteQF/>
<w:LidThemeOther>FR</w:LidThemeOther>
<w:LidThemeAsian>X-NONE</w:LidThemeAsian>
<w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript>
<w:Compatibility>
<w:BreakWrappedTables/>
<w:SnapToGridInCell/>
<w:WrapTextWithPunct/>
<w:UseAsianBreakRules/>
<w:DontGrowAutofit/>
<w:SplitPgBreakAndParaMark/>
<w:DontVertAlignCellWithSp/>
<w:DontBreakConstrainedForcedTables/>
<w:DontVertAlignInTxbx/>
<w:Word11KerningPairs/>
<w:CachedColBalance/>
</w:Compatibility>
<w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel>
<m:mathPr>
<m:mathFont m:val="Cambria Math"/>
<m:brkBin m:val="before"/>
<m:brkBinSub m:val="--"/>
<m:smallFrac m:val="off"/>
<m:dispDef/>
<m:lMargin m:val="0"/>
<m:rMargin m:val="0"/>
<m:defJc m:val="centerGroup"/>
<m:wrapIndent m:val="1440"/>
<m:intLim m:val="subSup"/>
<m:naryLim m:val="undOvr"/>
</m:mathPr></w:WordDocument>
</xml><![endif]--><!--[if gte mso 9]><xml>
<w:LatentStyles DefLockedState="false" DefUnhideWhenUsed="true"
DefSemiHidden="true" DefQFormat="false" DefPriority="99"
LatentStyleCount="267">
<w:LsdException Locked="false" Priority="0" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Normal"/>
<w:LsdException Locked="false" Priority="9" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="heading 1"/>
<w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 2"/>
<w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 3"/>
<w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 4"/>
<w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 5"/>
<w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 6"/>
<w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 7"/>
<w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 8"/>
<w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 9"/>
<w:LsdException Locked="false" Priority="39" Name="toc 1"/>
<w:LsdException Locked="false" Priority="39" Name="toc 2"/>
<w:LsdException Locked="false" Priority="39" Name="toc 3"/>
<w:LsdException Locked="false" Priority="39" Name="toc 4"/>
<w:LsdException Locked="false" Priority="39" Name="toc 5"/>
<w:LsdException Locked="false" Priority="39" Name="toc 6"/>
<w:LsdException Locked="false" Priority="39" Name="toc 7"/>
<w:LsdException Locked="false" Priority="39" Name="toc 8"/>
<w:LsdException Locked="false" Priority="39" Name="toc 9"/>
<w:LsdException Locked="false" Priority="35" QFormat="true" Name="caption"/>
<w:LsdException Locked="false" Priority="10" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Title"/>
<w:LsdException Locked="false" Priority="1" Name="Default Paragraph Font"/>
<w:LsdException Locked="false" Priority="11" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Subtitle"/>
<w:LsdException Locked="false" Priority="22" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Strong"/>
<w:LsdException Locked="false" Priority="20" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Emphasis"/>
<w:LsdException Locked="false" Priority="59" SemiHidden="false"
UnhideWhenUsed="false" Name="Table Grid"/>
<w:LsdException Locked="false" UnhideWhenUsed="false" Name="Placeholder Text"/>
<w:LsdException Locked="false" Priority="1" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="No Spacing"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading Accent 1"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List Accent 1"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid Accent 1"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1 Accent 1"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2 Accent 1"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1 Accent 1"/>
<w:LsdException Locked="false" UnhideWhenUsed="false" Name="Revision"/>
<w:LsdException Locked="false" Priority="34" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="List Paragraph"/>
<w:LsdException Locked="false" Priority="29" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Quote"/>
<w:LsdException Locked="false" Priority="30" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Intense Quote"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2 Accent 1"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1 Accent 1"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2 Accent 1"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3 Accent 1"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List Accent 1"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading Accent 1"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List Accent 1"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid Accent 1"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading Accent 2"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List Accent 2"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid Accent 2"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1 Accent 2"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2 Accent 2"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1 Accent 2"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2 Accent 2"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1 Accent 2"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2 Accent 2"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3 Accent 2"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List Accent 2"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading Accent 2"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List Accent 2"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid Accent 2"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading Accent 3"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List Accent 3"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid Accent 3"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1 Accent 3"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2 Accent 3"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1 Accent 3"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2 Accent 3"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1 Accent 3"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2 Accent 3"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3 Accent 3"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List Accent 3"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading Accent 3"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List Accent 3"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid Accent 3"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading Accent 4"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List Accent 4"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid Accent 4"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1 Accent 4"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2 Accent 4"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1 Accent 4"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2 Accent 4"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1 Accent 4"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2 Accent 4"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3 Accent 4"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List Accent 4"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading Accent 4"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List Accent 4"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid Accent 4"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading Accent 5"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List Accent 5"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid Accent 5"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1 Accent 5"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2 Accent 5"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1 Accent 5"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2 Accent 5"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1 Accent 5"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2 Accent 5"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3 Accent 5"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List Accent 5"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading Accent 5"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List Accent 5"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid Accent 5"/>
<w:LsdException Locked="false" Priority="60" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Shading Accent 6"/>
<w:LsdException Locked="false" Priority="61" SemiHidden="false"
UnhideWhenUsed="false" Name="Light List Accent 6"/>
<w:LsdException Locked="false" Priority="62" SemiHidden="false"
UnhideWhenUsed="false" Name="Light Grid Accent 6"/>
<w:LsdException Locked="false" Priority="63" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 1 Accent 6"/>
<w:LsdException Locked="false" Priority="64" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Shading 2 Accent 6"/>
<w:LsdException Locked="false" Priority="65" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 1 Accent 6"/>
<w:LsdException Locked="false" Priority="66" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium List 2 Accent 6"/>
<w:LsdException Locked="false" Priority="67" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 1 Accent 6"/>
<w:LsdException Locked="false" Priority="68" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 2 Accent 6"/>
<w:LsdException Locked="false" Priority="69" SemiHidden="false"
UnhideWhenUsed="false" Name="Medium Grid 3 Accent 6"/>
<w:LsdException Locked="false" Priority="70" SemiHidden="false"
UnhideWhenUsed="false" Name="Dark List Accent 6"/>
<w:LsdException Locked="false" Priority="71" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Shading Accent 6"/>
<w:LsdException Locked="false" Priority="72" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful List Accent 6"/>
<w:LsdException Locked="false" Priority="73" SemiHidden="false"
UnhideWhenUsed="false" Name="Colorful Grid Accent 6"/>
<w:LsdException Locked="false" Priority="19" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Subtle Emphasis"/>
<w:LsdException Locked="false" Priority="21" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Intense Emphasis"/>
<w:LsdException Locked="false" Priority="31" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Subtle Reference"/>
<w:LsdException Locked="false" Priority="32" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Intense Reference"/>
<w:LsdException Locked="false" Priority="33" SemiHidden="false"
UnhideWhenUsed="false" QFormat="true" Name="Book Title"/>
<w:LsdException Locked="false" Priority="37" Name="Bibliography"/>
<w:LsdException Locked="false" Priority="39" QFormat="true" Name="TOC Heading"/>
</w:LatentStyles>
</xml><![endif]-->
<style>
<!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;
mso-font-charset:0;
mso-generic-font-family:roman;
mso-font-pitch:variable;
mso-font-signature:-536870145 1107305727 0 0 415 0;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;
mso-font-charset:0;
mso-generic-font-family:swiss;
mso-font-pitch:variable;
mso-font-signature:-520092929 1073786111 9 0 415 0;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{mso-style-unhide:no;
mso-style-qformat:yes;
mso-style-parent:"";
margin-top:0cm;
margin-right:0cm;
margin-bottom:10.0pt;
margin-left:0cm;
line-height:115%;
mso-pagination:widow-orphan;
font-size:11.0pt;
font-family:"Calibri","sans-serif";
mso-ascii-font-family:Calibri;
mso-ascii-theme-font:minor-latin;
mso-fareast-font-family:Calibri;
mso-fareast-theme-font:minor-latin;
mso-hansi-font-family:Calibri;
mso-hansi-theme-font:minor-latin;
mso-bidi-font-family:"Times New Roman";
mso-bidi-theme-font:minor-bidi;
mso-fareast-language:EN-US;}
.MsoChpDefault
{mso-style-type:export-only;
mso-default-props:yes;
mso-ascii-font-family:Calibri;
mso-ascii-theme-font:minor-latin;
mso-fareast-font-family:Calibri;
mso-fareast-theme-font:minor-latin;
mso-hansi-font-family:Calibri;
mso-hansi-theme-font:minor-latin;
mso-bidi-font-family:"Times New Roman";
mso-bidi-theme-font:minor-bidi;
mso-fareast-language:EN-US;}
.MsoPapDefault
{mso-style-type:export-only;
margin-bottom:10.0pt;
line-height:115%;}
@page Section1
{size:612.0pt 792.0pt;
margin:70.85pt 70.85pt 70.85pt 70.85pt;
mso-header-margin:36.0pt;
mso-footer-margin:36.0pt;
mso-paper-source:0;}
div.Section1
{page:Section1;}
/* List Definitions */
@list l0
{mso-list-id:1874493137;
mso-list-template-ids:1610103466;}
@list l0:level1
{mso-level-number-format:bullet;
mso-level-text:;
mso-level-tab-stop:36.0pt;
mso-level-number-position:left;
text-indent:-18.0pt;
mso-ansi-font-size:10.0pt;
font-family:Symbol;}
ol
{margin-bottom:0cm;}
ul
{margin-bottom:0cm;}
-->
</style><!--[if gte mso 10]>
<style>
/* Style Definitions */
table.MsoNormalTable
{mso-style-name:"Tableau Normal";
mso-tstyle-rowband-size:0;
mso-tstyle-colband-size:0;
mso-style-noshow:yes;
mso-style-priority:99;
mso-style-qformat:yes;
mso-style-parent:"";
mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
mso-para-margin-top:0cm;
mso-para-margin-right:0cm;
mso-para-margin-bottom:10.0pt;
mso-para-margin-left:0cm;
line-height:115%;
mso-pagination:widow-orphan;
font-size:11.0pt;
font-family:"Calibri","sans-serif";
mso-ascii-font-family:Calibri;
mso-ascii-theme-font:minor-latin;
mso-hansi-font-family:Calibri;
mso-hansi-theme-font:minor-latin;
mso-bidi-font-family:"Times New Roman";
mso-bidi-theme-font:minor-bidi;
mso-fareast-language:EN-US;}
</style>
<![endif]-->
<p class="MsoNormal"><span
style="font-size:12.0pt;line-height:115%;
font-family:"Times New
Roman","serif";mso-ansi-language:EN-US"
lang="EN-US">Thank you <span
style="color:black;background:white">Michael <span
style="mso-spacerun:yes"> </span>for your response. <o:p></o:p></span></span></p>
<p class="MsoNormal"><span
style="font-size:12.0pt;line-height:115%;
font-family:"Times New
Roman","serif";color:black;background:white;mso-ansi-language:EN-US"
lang="EN-US">As suggested by you, I would use Augustus and<span
style="mso-spacerun:yes"> </span>Snap trained both by the
assembled
transcripts in a bootstrap fashion.<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:12.0pt;line-height:115%;
font-family:"Times New
Roman","serif";color:black;background:white;mso-ansi-language:EN-US"
lang="EN-US">For the masking, I intend to to adapt <span
style="mso-spacerun:yes"> </span>Carson</span> strategy :<o:p></o:p></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
margin-left:19.2pt;text-indent:-18.0pt;line-height:18.0pt;mso-list:l0
level1 lfo1;
tab-stops:list 36.0pt;background:white"><!--[if !supportLists]--><span
style="font-size:10.0pt;mso-bidi-font-size:12.0pt;font-family:Symbol;
mso-fareast-font-family:Symbol;mso-bidi-font-family:Symbol;color:black;
mso-ansi-language:EN-US;mso-fareast-language:FR" lang="EN-US"><span
style="mso-list:Ignore">·<span style="font:7.0pt "Times
New Roman"">
</span></span></span><!--[endif]--><span
style="font-size:12.0pt;
font-family:"Times New
Roman","serif";mso-fareast-font-family:"Times
New Roman";
color:black;mso-ansi-language:EN-US;mso-fareast-language:FR"
lang="EN-US">Collecting
RepeatModeler repeats.lib<o:p></o:p></span></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
margin-left:19.2pt;text-indent:-18.0pt;line-height:18.0pt;mso-list:l0
level1 lfo1;
tab-stops:list 36.0pt;background:white"><!--[if !supportLists]--><span
style="font-size:10.0pt;mso-bidi-font-size:12.0pt;font-family:Symbol;
mso-fareast-font-family:Symbol;mso-bidi-font-family:Symbol;mso-ansi-language:
EN-US" lang="EN-US"><span style="mso-list:Ignore">·<span
style="font:7.0pt "Times New Roman"">
</span></span></span><!--[endif]--><span
style="font-size:12.0pt;
font-family:"Times New
Roman","serif";mso-fareast-font-family:"Times
New Roman";
mso-ansi-language:EN-US;mso-fareast-language:FR" lang="EN-US">Searching
Sequences in
Modelerunknown.lib <span style="mso-spacerun:yes"> </span>against
a transposase
database (derived from </span><span
style="font-size:12.0pt;font-family:
"Times New
Roman","serif";mso-fareast-font-family:"Times
New Roman";mso-fareast-language:
FR"><a href="http://www.repeatmasker.org/"><span
style="color:windowtext;
mso-ansi-language:EN-US;text-decoration:none;text-underline:none"
lang="EN-US">RepeatMasker</span></a></span><span
style="font-size:12.0pt;font-family:"Times New
Roman","serif";
mso-fareast-font-family:"Times New
Roman";mso-ansi-language:EN-US;mso-fareast-language:
FR" lang="EN-US"> package and </span><span
style="font-size:12.0pt;font-family:
"Times New
Roman","serif";mso-fareast-font-family:"Times
New Roman";mso-fareast-language:
FR"><a href="http://www.ncbi.nlm.nih.gov/pubmed/21535899"><span
style="color:windowtext;mso-ansi-language:EN-US;text-decoration:none;
text-underline:none" lang="EN-US">Kennedy et al (2011)</span></a></span><span
style="font-size:12.0pt;font-family:"Times New
Roman","serif";mso-fareast-font-family:
"Times New
Roman";mso-ansi-language:EN-US;mso-fareast-language:FR"
lang="EN-US">) and <span style="mso-spacerun:yes"> </span>considering
sequences matching <span style="mso-spacerun:yes"> </span>transposases
as transposons.</span><span
style="font-size:12.0pt;font-family:"Times New
Roman","serif";
mso-ansi-language:EN-US" lang="EN-US"><o:p></o:p></span></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
margin-left:19.2pt;text-indent:-18.0pt;line-height:18.0pt;mso-list:l0
level1 lfo1;
tab-stops:list 36.0pt;background:white"><!--[if !supportLists]--><span
style="font-size:10.0pt;mso-bidi-font-size:12.0pt;font-family:Symbol;
mso-fareast-font-family:Symbol;mso-bidi-font-family:Symbol;mso-ansi-language:
EN-US" lang="EN-US"><span style="mso-list:Ignore">·<span
style="font:7.0pt "Times New Roman"">
</span></span></span><!--[endif]--><span
style="font-size:12.0pt;
font-family:"Times New
Roman","serif";mso-ansi-language:EN-US"
lang="EN-US">Exclusion of
gene fragments in both known and unkown repeats<o:p></o:p></span></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
margin-left:19.2pt;text-indent:-18.0pt;line-height:18.0pt;mso-list:l0
level1 lfo1;
tab-stops:list 36.0pt;background:white"><!--[if !supportLists]--><span
style="font-size:10.0pt;mso-bidi-font-size:12.0pt;font-family:Symbol;
mso-fareast-font-family:Symbol;mso-bidi-font-family:Symbol;mso-ansi-language:
EN-US" lang="EN-US"><span style="mso-list:Ignore">·<span
style="font:7.0pt "Times New Roman"">
</span></span></span><!--[endif]--><span
style="font-size:12.0pt;
font-family:"Times New
Roman","serif";mso-ansi-language:EN-US"
lang="EN-US">As I'm concerned
by gene duplications, the remainder sequences in the unkown <span
style="mso-spacerun:yes"> </span>lib present less than 10
times will be
removed.<o:p></o:p></span></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
margin-left:19.2pt;line-height:18.0pt;background:white"><span
style="font-size:12.0pt;font-family:"Times New
Roman","serif";mso-ansi-language:
EN-US" lang="EN-US"><br>
</span></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
margin-left:19.2pt;line-height:18.0pt;background:white"><span
style="font-size:12.0pt;font-family:"Times New
Roman","serif";mso-ansi-language:
EN-US" lang="EN-US">Thank you again for your time and I remain
open to any suggestion.<o:p></o:p></span></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
margin-left:19.2pt;line-height:18.0pt;background:white"><span
style="font-size:12.0pt;font-family:"Times New
Roman","serif";mso-ansi-language:
EN-US" lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"
style="mso-margin-top-alt:auto;margin-bottom:1.2pt;
margin-left:19.2pt;line-height:18.0pt;background:white"><span
style="font-size:12.0pt;font-family:"Times New
Roman","serif";mso-ansi-language:
EN-US" lang="EN-US">Best,<o:p></o:p></span></p>
<span
style="font-size:12.0pt;line-height:115%;font-family:"Times
New Roman","serif";
mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-ansi-language:
EN-US;mso-fareast-language:EN-US;mso-bidi-language:AR-SA"
lang="EN-US">Amine</span></p>
<br>
<div class="moz-cite-prefix">Le 27/10/2016 à 15:21, Michael Campbell
a écrit :<br>
</div>
<blockquote
cite="mid:8935E6BD-FDEC-464B-B174-94649CB42D63@gmail.com"
type="cite">
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
I think that if you train any further you will run the risk of
overtraining. setting alt_splice to 1 will add transcripts but not
genes, so the gene count is going to be related to the training of
the gene finder. I would recommend looking at a few of your large
scaffolds in a genome browser. I would also recommend adding a
second gene predictor such as augustus. When multiple predictors
are used and the models they predict converge you can have more
confidence in the gene prediction.
<div class=""><br class="">
</div>
<div class="">For the masking you can make a species specific
repeat library like Carson suggested to see if the gene count
comes down a little. If you are concerned about masking
duplicated genes you cad do a couple of things. You can filter
the repeat library based on known proteins. You can also set a
copy number minimum for the making and only include repeats that
are present more than 10 time in the genome. Here are a couple
of URLs for making species specific repeat libraries </div>
<div class=""><a moz-do-not-send="true"
href="http://weatherby.genetics.utah.edu/MAKER/wiki/index.php/Repeat_Library_Construction-Advanced"
class="">http://weatherby.genetics.utah.edu/MAKER/wiki/index.php/Repeat_Library_Construction-Advanced</a></div>
<div class=""><a moz-do-not-send="true"
href="http://weatherby.genetics.utah.edu/MAKER/wiki/index.php/Repeat_Library_Construction-Basic"
class="">http://weatherby.genetics.utah.edu/MAKER/wiki/index.php/Repeat_Library_Construction-Basic</a></div>
<div class=""><br class="">
</div>
<div class="">Take care,</div>
<div class="">Mike</div>
<div class=""> <br class="">
<div>
<blockquote type="cite" class="">
<div class="">On Oct 27, 2016, at 5:54 AM, Mohamed Amine
CHEBBI <<a moz-do-not-send="true"
href="mailto:mohamed.amine.chebbi@univ-poitiers.fr"
class="">mohamed.amine.chebbi@univ-poitiers.fr</a>>
wrote:</div>
<br class="Apple-interchange-newline">
<div class="">
<div style="font-family: Helvetica; font-size: 18px;
font-style: normal; font-variant: normal; font-weight:
normal; letter-spacing: normal; line-height: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);" class=""><br
class="webkit-block-placeholder">
</div>
<div style="font-family: Helvetica; font-size: 18px;
font-style: normal; font-variant: normal; font-weight:
normal; letter-spacing: normal; line-height: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);" class=""><br
class="webkit-block-placeholder">
</div>
<div style="font-family: Helvetica; font-size: 18px;
font-style: normal; font-variant: normal; font-weight:
normal; letter-spacing: normal; line-height: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);" class=""><br
class="webkit-block-placeholder">
</div>
<p class="MsoNormal" style="margin: 0cm 0cm 10pt;
line-height: normal; font-size: 11pt; font-family:
Calibri, sans-serif; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);"><span
style="font-size: 14pt;" class="" lang="EN-US">Sorry,
the X and Y were switched in the plot due to a
mishandling. Please find attached now the correct AED
graph.<span class="Apple-converted-space"> </span><o:p
class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0cm 0cm 10pt;
line-height: normal; font-size: 11pt; font-family:
Calibri, sans-serif; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);"><span
style="font-size: 14pt;" class="" lang="EN-US">The
round 3 (red curve) shows little higher overall AED
than the second round (green curve) and more genes (<span
style="color: rgb(33, 33, 33); background-color:
white; background-position: initial initial;
background-repeat: initial initial;" class="">22931
comparing to 22547 in the round 2). Do you think
that I should stop at the second round ?</span><o:p
class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0cm 0cm 10pt;
line-height: normal; font-size: 11pt; font-family:
Calibri, sans-serif; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);"><span
style="font-size: 14pt;" class="" lang="EN-US">I
didn't<span class=""> <span
class="Apple-converted-space"> </span></span>precise
in the precedent email that the Repeat masking was
done in Maker using the Repbase and only models found
by RepeatModeler having identities. I let<span
class=""> <span class="Apple-converted-space"> </span></span>unmasked
the unkown lib of RepeatModeler. In fact we expect a
high rate of segmental and gene duplication in the
genome and then we could explain the high overall
count of genes found by Maker.<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0cm 0cm 10pt;
line-height: normal; font-size: 11pt; font-family:
Calibri, sans-serif; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);"><span
style="font-size: 14pt;" class="" lang="EN-US">In the
other hand the high, rate of genes may be also
expalined by the fact that I activate the alt_splice=1
option to find alternative splicing, do you think that
it was a good idea ?<o:p class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0cm 0cm 10pt;
line-height: normal; font-size: 11pt; font-family:
Calibri, sans-serif; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);"><span
style="font-size: 14pt;" class="" lang="EN-US"> <o:p
class=""></o:p></span></p>
<p class="MsoNormal" style="margin: 0cm 0cm 10pt;
line-height: normal; font-size: 11pt; font-family:
Calibri, sans-serif; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);"><span
style="font-size: 14pt;" class="" lang="EN-US">Thank
you very much for your time.<span
class="Apple-converted-space"> </span><o:p class=""></o:p></span></p>
<br style="font-family: Helvetica; font-size: 18px;
font-style: normal; font-variant: normal; font-weight:
normal; letter-spacing: normal; line-height: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);" class="">
<div style="font-family: Helvetica; font-size: 18px;
font-style: normal; font-variant: normal; font-weight:
normal; letter-spacing: normal; line-height: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);" class=""><br
class="webkit-block-placeholder">
</div>
<p style="font-family: Helvetica; font-size: 18px;
font-style: normal; font-variant: normal; font-weight:
normal; letter-spacing: normal; line-height: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);" class=""><span
class="" lang="EN-US">Best,</span></p>
<p class="MsoNormal" style="margin: 0cm 0cm 10pt;
line-height: 16.866666793823242px; font-size: 11pt;
font-family: Calibri, sans-serif; font-style: normal;
font-variant: normal; font-weight: normal;
letter-spacing: normal; orphans: auto; text-align:
start; text-indent: 0px; text-transform: none;
white-space: normal; widows: auto; word-spacing: 0px;
-webkit-text-stroke-width: 0px; background-color:
rgb(255, 255, 255);">Amine<br class="">
<span class="" lang="EN-US"><o:p class=""></o:p></span></p>
<div style="font-family: Helvetica; font-size: 18px;
font-style: normal; font-variant: normal; font-weight:
normal; letter-spacing: normal; line-height: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);" class=""><br
class="webkit-block-placeholder">
</div>
<br style="font-family: Helvetica; font-size: 18px;
font-style: normal; font-variant: normal; font-weight:
normal; letter-spacing: normal; line-height: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);" class="">
<div class="moz-cite-prefix" style="font-family:
Helvetica; font-size: 18px; font-style: normal;
font-variant: normal; font-weight: normal;
letter-spacing: normal; line-height: normal; orphans:
auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);">Le 26/10/2016 à
20:06, Carson Holt a écrit :<br class="">
</div>
<blockquote
cite="mid:3EA2EC84-9B2A-4631-97F8-44D774E67468@gmail.com"
type="cite" style="font-family: Helvetica; font-size:
18px; font-style: normal; font-variant: normal;
font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space:
normal; widows: auto; word-spacing: 0px;
-webkit-text-stroke-width: 0px; background-color:
rgb(255, 255, 255);" class="">Sorry. I also assumed X
and Y was flipped when I looked at it. Now I read the
labels, your AED curve would be weird unless the X and Y
are flipped in your figure.
<div class=""><br class="">
</div>
<div class="">—Carson</div>
<div class=""><br class="">
<div class=""><br class="">
<div class="">
<blockquote type="cite" class="">
<div class="">On Oct 26, 2016, at 12:04 PM,
Carson Holt <<a moz-do-not-send="true"
href="mailto:carsonhh@gmail.com" class="">carsonhh@gmail.com</a>>
wrote:</div>
<br class="Apple-interchange-newline">
<div class="">
<div class="" style="word-wrap: break-word;
-webkit-nbsp-mode: space;
-webkit-line-break: after-white-space;">Your
AED curve looks fine. The first run (using
protein2genome or est2genome I assume) will
always have really low overall AED because
they are exact copies of the
protein/transcript alignments (so AED is
meaningless there because it will always
artificially look good). The protein2genome
or est2genome modles also have a hard
end-to-end coverage filtering cutoff of 0.5
when generated (apparent in the curve -
value in maker_bopts.ctl). The next runs
with SNAP show >80% of models with AED
under 0.5, so it looks good. You can further
look at models by adding protein domains
using InterProScan in which you would expect
70-80% of models to contain a recognizable
InterPro domain (false and bad models will
result in very low overall domain content).
<div class=""><br class="">
</div>
<div class="">Your overall gene counts are a
little high though for an arthropod
(14,000-19,000 genes would be expected as
gene loss rather than gene gain is the
primary evolutionary force in the
Ecdysozoa). However your gene counts can
be explained by either insufficient repeat
masking (you can add a RepeatModeler
generated library to the existing settings
to help with this), poor mRNA-seq assembly
or a lot of noise in the RNA-seq (this can
be helped with more strict assembly
parameters including the jaccard-clip
option in trinity), or it is just the
result of assembly fragmentation (if you
have a lot of contigs or runs of NNNN in
the assembly, then many genes will be
split which results in inflated gene
counts).<br class="">
<div class=""><br class="">
</div>
<div class="">Finally manually look at the
most gene dense contigs in a browser
like Apollo or IGV (gene_density =
gene_count / contig_length). If the most
gene dense contigs are overwhelmingly
single exon, then you may need to filter
out some prokaryotic assembly
contamination (not uncommon). If you
have contamination, it will assemble as
independent contigs, so is easily
blacklisted and can be identified
visually (always gene dense and single
exon).</div>
<div class=""><br class="">
</div>
<div class="">Thanks,</div>
<div class="">Carson</div>
<div class=""><br class="">
</div>
<div class=""><br class="">
</div>
<div class=""><br class="">
</div>
<div class=""><br class="">
<div class="">
<blockquote type="cite" class="">
<div class="">On Oct 26, 2016, at
7:09 AM, Mohamed Amine Chebbi <<a
moz-do-not-send="true"
class="moz-txt-link-abbreviated"
href="mailto:mohamed.amine.chebbi@univ-poitiers.fr"><a class="moz-txt-link-abbreviated" href="mailto:mohamed.amine.chebbi@univ-poitiers.fr">mohamed.amine.chebbi@univ-poitiers.fr</a></a>>
wrote:</div>
<br
class="Apple-interchange-newline">
<div class="">
<div class="">
<div class=""
style="font-family: arial,
helvetica, sans-serif;
font-size: 12pt;">
<div class="">
<div class="" style="margin:
0px; line-height: normal;"><span
data-mce-style="font-size: 12.0pt; font-family: 'Times New
Roman','serif'; color:
#222222; background:
white;" class=""
style="font-size: 12pt;
color: rgb(34, 34, 34);
background-color: white;
background-position:
initial initial;
background-repeat:
initial initial;">Hi !</span></div>
<div class=""
style="line-height:
normal; background-color:
white; margin: 0px;"><span
data-mce-style="font-size: 12.0pt; font-family: 'Times New
Roman','serif'; color:
#222222;" class=""
style="font-size: 12pt;">I
have tried three rounds
of annotation in Maker
on a non model arthropod
genome (1.7Gb) which is
an hybrid assembly of
Pacbio and illumina
reads.</span></div>
<div class=""
style="line-height:
normal; background-color:
white; margin: 0px;"><span
data-mce-style="font-size: 12.0pt; font-family: 'Times New
Roman','serif'; color:
#222222;" class=""
style="font-size: 12pt;">As
suggested in the
tutorial, I ran in the
first round Maker with
repeat masking to
generate gene models
using transcript
(Trinity assembly) and
protein (swissprot)
evidence. Then Maker
models were used twice
in a bootstrap fashion
to retrain SNAP. </span></div>
<div class=""
style="line-height:
normal; background-color:
white; margin: 0px;"><span
data-mce-style="font-size: 12.0pt; font-family: 'Times New
Roman','serif'; color:
#222222;" class=""
style="font-size: 12pt;">The
number of genes drops
from<span
class="Apple-converted-space"> </span></span><span
data-mce-style="font-size: 12.0pt; font-family: 'Times New
Roman','serif'; color:
#212121; background:
white;" class=""
style="font-size: 12pt;
color: rgb(33, 33, 33);
background-color: white;
background-position:
initial initial;
background-repeat:
initial initial;">29207
in the round 1 to 22547
in the round 2 then
increases slightly to
22931 in the round 3.</span></div>
<div class=""
style="line-height:
normal; background-color:
white; margin: 0px;"><br
class="">
<span
data-mce-style="font-size:
12.0pt; font-family:
'Times New
Roman','serif'; color:
#212121; background:
white;" class=""
style="font-size: 12pt;
color: rgb(33, 33, 33);
background-color: white;
background-position:
initial initial;
background-repeat:
initial initial;"><span data-mce-style="font-variant-ligatures: normal; font-variant-caps: normal; orphans: 2; white-space: pre-wrap; widows: 2; -webkit-text-stroke-width: 0px; float: none; word-spacing: 0px;" class="" style="orphans: 2; white-space: pre-wrap; widows: 2; -webkit-text-stroke-width: 0px; float: none; word-spacing: 0px;">However, the AED profile (attached) don't seem to be satisfactory.</span></span></div>
<div class=""
style="line-height:
normal; background-color:
white; margin: 0px;"><span
data-mce-style="font-size: 12.0pt; font-family: 'Times New
Roman','serif'; color:
#212121; background:
white;" class=""
style="font-size: 12pt;
color: rgb(33, 33, 33);
background-color: white;
background-position:
initial initial;
background-repeat:
initial initial;">So I
wonder if you could let
me a good strategy to
improve the annotation
quality. Do you think
that filtering good
transcripts could
improve results. If yes
, which criteria should</span><span
data-mce-style="font-size: 12.0pt; font-family: 'Times New
Roman','serif'; color:
#222222;" class=""
style="font-size: 12pt;"><span
class="Apple-converted-space"> </span>be taken into account ?</span></div>
<div class=""
style="line-height:
normal; background-color:
white; margin: 0px;"><span
data-mce-style="font-size: 12.0pt; font-family: 'Times New
Roman','serif'; color:
#222222;" class=""
style="font-size: 12pt;">Thank
you.</span></div>
<div class=""
style="line-height:
normal; background-color:
white; margin: 0px;"><span
data-mce-style="font-size: 12.0pt; font-family: 'Times New
Roman','serif'; color:
#222222;" class=""
style="font-size: 12pt;"><br
data-mce-bogus="1"
class="">
</span></div>
<div class=""
style="line-height:
normal; background-color:
white; margin: 0px;"><span
data-mce-style="font-size: 12.0pt; font-family: 'Times New
Roman','serif'; color:
#222222;" class=""
style="font-size: 12pt;">Best;</span></div>
<div class=""
style="line-height:
normal; background-color:
white; margin: 0px;"><span
data-mce-style="font-size: 12.0pt; font-family: 'Times New
Roman','serif'; color:
#222222;" class=""
style="font-size: 12pt;">Amine</span></div>
</div>
</div>
</div>
<span
id="cid:A35AEA9C-A0B8-490E-84CF-C956A8207A2C@genetics.utah.edu"
class=""><AED-Graph.pdf></span>_______________________________________________<br
class="">
maker-devel mailing list<br
class="">
<a moz-do-not-send="true"
href="mailto:maker-devel@box290.bluehost.com"
class="">maker-devel@box290.bluehost.com</a><br
class="">
<a moz-do-not-send="true"
href="http://box290.bluehost.com/mailman/listinfo/maker-devel_yandell-lab.org"
class="">http://box290.bluehost.com/mailman/listinfo/maker-devel_yandell-lab.org</a><br
class="">
</div>
</blockquote>
</div>
<br class="">
</div>
</div>
</div>
</div>
</blockquote>
</div>
<br class="">
</div>
</div>
</blockquote>
<br style="font-family: Helvetica; font-size: 18px;
font-style: normal; font-variant: normal; font-weight:
normal; letter-spacing: normal; line-height: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;
background-color: rgb(255, 255, 255);" class="">
<pre class="moz-signature" cols="72" style="font-size: 18px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);">--
Mohamed Amine CHEBBI, PhD Student
Université de Poitiers
Laboratoire Ecologie et Biologie des Interactions - UMR CNRS 7267
Equipe Ecologie Evolution Symbiose
Bât. B8-B35 - 5 Rue Albert Turpin
TSA 51106
F-86022 Poitiers Cedex 9
FRANCE
Lab website: <a moz-do-not-send="true" class="moz-txt-link-freetext" href="http://ecoevol.labo.univ-poitiers.fr/">http://ecoevol.labo.univ-poitiers.fr/</a></pre>
<span id="cid:A1915C92-1E48-4258-A2BE-A5E560F53C31"><AED-Graph.pdf></span></div>
</blockquote>
</div>
<br class="">
</div>
</blockquote>
<br>
<pre class="moz-signature" cols="72">--
Mohamed Amine CHEBBI, PhD Student
Université de Poitiers
Laboratoire Ecologie et Biologie des Interactions - UMR CNRS 7267
Equipe Ecologie Evolution Symbiose
Bât. B8-B35 - 5 Rue Albert Turpin
TSA 51106
F-86022 Poitiers Cedex 9
FRANCE
Lab website: <a class="moz-txt-link-freetext" href="http://ecoevol.labo.univ-poitiers.fr/">http://ecoevol.labo.univ-poitiers.fr/</a></pre>
</body>
</html>