<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi Maker develop team!<div class=""><br class=""></div><div class="">I am trying to figure the second column of gff file generated by maker, which should be the source of this annotation. Besides of what the tutorial lists as,</div><div class=""><br class=""></div><div class=""><div style="margin: 0.5em 0px; line-height: inherit; color: rgb(37, 37, 37); font-family: 'Helvetica Neue', Helvetica, Roboto, Arial, sans-serif; font-size: 14px;" class="">Possible Sources Include:</div><ul style="line-height: 1.5em; margin: 0.3em 0px 0px 1.6em; padding: 0px; list-style-image: url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAUAAAANAQMAAABb8jbLAAAABlBMVEX///8AUow5QSOjAAAAAXRSTlMAQObYZgAAABNJREFUCB1jYEABBQw/wLCAgQEAGpIDyT0IVcsAAAAASUVORK5CYII=); color: rgb(37, 37, 37); font-family: 'Helvetica Neue', Helvetica, Roboto, Arial, sans-serif; font-size: 14px;" class=""><li style="margin-bottom: 0.1em;" class="">BLASTN - BLASTN alignment of EST evidence</li><li style="margin-bottom: 0.1em;" class="">BLASTX - BLASTX alignment of protein evidence</li><li style="margin-bottom: 0.1em;" class="">TBLASTX - TBLASTX alignment of EST evidence from closely related organisms</li><li style="margin-bottom: 0.1em;" class="">EST2Genome - Polished EST alignment from Exonerate</li><li style="margin-bottom: 0.1em;" class="">Protein2Genome - Polished protein alignment from Exonerate</li><li style="margin-bottom: 0.1em;" class="">SNAP - SNAP <i class="">ab inito</i> gene prediction</li><li style="margin-bottom: 0.1em;" class="">GENEMARK - GeneMark<i class="">ab inito</i> gene prediction</li><li style="margin-bottom: 0.1em;" class="">Augustus - Augustus <i class="">ab inito</i> gene prediction</li><li style="margin-bottom: 0.1em;" class="">FgenesH - FGENESH <i class="">ab inito</i> gene prediction</li><li style="margin-bottom: 0.1em;" class="">Repeatmasker - RepeatMasker identified repeat</li><li style="margin-bottom: 0.1em;" class="">RepeatRunner - RepeatRunner identified repeat from the repeat protein database</li><li style="margin-bottom: 0.1em;" class="">tRNAScan - tRNAScan-SE tRNA predictions (coming soon)</li><li style="margin-bottom: 0.1em;" class="">PASA - PASA gene predictions (coming soon)</li></ul><div class=""><br class=""></div></div><div class="">There are other sources that I noticed from my gff file, like cdna2genome. Is there any other detailed documentation explaining such sources besides of those listed above?</div><div class=""><br class=""></div><div class="">Thanks</div><div class="">Qihua</div><div class=""><br class=""></div></body></html>