<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div class="">So you are running the test data job correct? So if you get any error with the test data job, something is wrong with your installation.</div><div class=""><br class=""></div>Make sure BioPerl is up to data (and the same everywhere). If using your own installation of Perl, make sure it had BerkleyDB support when you installed it (if you are using something like perlbrew , you may not have BerkleyDB support and this may affect BioPerl indexing). Also try reinstalling exonertate.<div class=""><br class=""></div><div class="">And make sure to delete any run directories before retrying.</div><div class=""><br class=""></div><div class="">—Carson<br class=""><div class=""><br class=""></div><div class=""><br class=""><div><blockquote type="cite" class=""><div class="">On Feb 22, 2017, at 11:03 AM, Seth Munholland <<a href="mailto:munholl@uwindsor.ca" class="">munholl@uwindsor.ca</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div dir="ltr" class=""><div class=""><div class="">After my clustering issue I figured I would go node by node and run MAKER locally on the test data set to be sure that it was working properly before trying it as a full MPI run. After adjusting all my exes to point to the NFS versions I am consistently getting the same error on each node when it comes time to run exonerate:<br class=""><br class="">polishig ESTs<br class="">running est2genome search.<br class="">#--------- command -------------#<br class="">Widget::exonerate::est2genome:<br class="">/Data/Apps/exonerate/src/program/exonerate -q /tmp/maker_q3n9fB/0/dpp-mRNA-5.for.26386-32056.0.fasta -t /tmp/maker_q3n9fB/0/contig-dpp-500-500.26386-32056.0.fasta -Q dna -T dna --model est2genome --minintron 20 --maxintron 10000 --showcigar --percent 20 > /tmp/maker_q3n9fB/0/contig-dpp-500-500.26386-32056.dpp-mRNA-5.e.exonerate<br class="">#-------------------------------#<br class="">Messed up acceptor:TT in Widget::exonerate::est2genome::get_acceptor!<br class="">--> rank=NA, hostname=beanblade2<br class="">ERROR: Failed while polishig ESTs<br class="">ERROR: Chunk failed at level:2, tier_type:3<br class="">FAILED CONTIG:contig-dpp-500-500<br class=""><br class="">ERROR: Chunk failed at level:4, tier_type:0<br class="">FAILED CONTIG:contig-dpp-500-500<br class=""><br class=""></div>The only results I find on google was an issue with an improper character in the est file, but a cat of the dpp_est.fasta shows nothing incorrect and since they all point to dpp-mRNA-5 I backed up the provided est and removed it. The response was:<br class=""><br class="">polishig ESTs<br class="">running est2genome search.<br class="">#--------- command -------------#<br class="">Widget::exonerate::est2genome:<br class="">/Data/Apps/exonerate/src/program/exonerate -q /tmp/maker_I7TZxt/0/dpp-mRNA-4.for.22889-32056.0.fasta -t /tmp/maker_I7TZxt/0/contig-dpp-500-500.22889-32056.0.fasta -Q dna -T dna --model est2genome --minintron 20 --maxintron 10000 --showcigar --percent 20 > /tmp/maker_I7TZxt/0/contig-dpp-500-500.22889-32056.dpp-mRNA-4.e.exonerate<br class="">#-------------------------------#<br class="">Messed up acceptor:TT in Widget::exonerate::est2genome::get_acceptor!<br class="">--> rank=NA, hostname=beanblade4<br class="">ERROR: Failed while polishig ESTs<br class="">ERROR: Chunk failed at level:2, tier_type:3<br class="">FAILED CONTIG:contig-dpp-500-500<br class=""><br class="">ERROR: Chunk failed at level:4, tier_type:0<br class="">FAILED CONTIG:contig-dpp-500-500<br class=""><br class=""></div>So the error seems to be pointing at something happening when wrapping up the ESTs.<br class=""><div class=""><div class=""><div class=""><br clear="all" class=""><div class=""><div class=""><div class="gmail_signature"><div dir="ltr" class=""><div class="">Seth Munholland, B.Sc.<br class=""></div><div class="">Department of Biological Sciences<br class="">
Rm. 304 Biology Building<br class="">
University of Windsor<br class="">
401 Sunset Ave. N9B 3P4<br class="">
T: <a value="+15192533000" class="">(519) 253-3000 Ext: 4755</a></div></div></div></div>
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