<div dir="ltr">I have now tried with multiple versions of blast (2.6 and 2.28 binaries and built from source) and get the same error:<div><br></div><div>
<p class="gmail-p1"><span class="gmail-s1">setting up GFF3 output and fasta chunks</span></p>
<p class="gmail-p1"><span class="gmail-s1">doing blastn of ESTs</span></p>
<p class="gmail-p1"><span class="gmail-s1">running blast search.</span></p>
<p class="gmail-p1"><span class="gmail-s1">#--------- command -------------#</span></p>
<p class="gmail-p1"><span class="gmail-s1">Widget::blastn:</span></p>
<p class="gmail-p1"><span class="gmail-s1">/home/mnaymik/TOOLS/ncbi-blast-2.2.28+/bin/blastn -db /scratch/mnaymik/maker/tmp/maker_cah</span></p>
<p class="gmail-p1"><span class="gmail-s1">#-------------------------------#</span></p>
<p class="gmail-p1"><span class="gmail-s1">Error: NCBI C++ Exception:</span></p>
<p class="gmail-p1"><span class="gmail-s1"> "/home/mnaymik/TOOLS/ncbi-blast-2.2.28+-src/c++/src/corelib/ncbiobj.cpp", line 925: Cr</span></p>
<p class="gmail-p2"><span class="gmail-s1"></span><br></p>
<p class="gmail-p1"><span class="gmail-s1">Error: NCBI C++ Exception:</span></p>
<p class="gmail-p1"><span class="gmail-s1"> "/home/mnaymik/TOOLS/ncbi-blast-2.2.28+-src/c++/src/corelib/ncbiobj.cpp", line 925: Cr</span></p>
<p class="gmail-p2"><span class="gmail-s1"></span><br></p>
<p class="gmail-p1"><span class="gmail-s1">examining contents of the fasta file and run log</span></p>
<p class="gmail-p1"><span class="gmail-s1">ERROR: BLASTN failed</span></p>
<p class="gmail-p1"><span class="gmail-s1">--> rank=87, hostname=pnap-pe7-s09</span></p>
<p class="gmail-p1"><span class="gmail-s1">ERROR: Failed while doing blastn of ESTs</span></p>
<p class="gmail-p1"><span class="gmail-s1">ERROR: Chunk failed at level:0, tier_type:3</span></p>
<p class="gmail-p1"><span class="gmail-s1">FAILED CONTIG:6537645</span></p>
<p class="gmail-p2"><span class="gmail-s1"></span><br></p>
<p class="gmail-p1"><span class="gmail-s1">ERROR: BLASTN failed</span></p>
<p class="gmail-p1"><span class="gmail-s1">--> rank=88, hostname=pnap-pe7-s09</span></p>
<p class="gmail-p1"><span class="gmail-s1">ERROR: Failed while doing blastn of ESTs</span></p>
<p class="gmail-p1"><span class="gmail-s1">ERROR: Chunk failed at level:0, tier_type:3</span></p>
<p class="gmail-p1"><span class="gmail-s1">FAILED CONTIG:6537659</span></p>
<p class="gmail-p2"><span class="gmail-s1"></span><br></p>
<p class="gmail-p1"><span class="gmail-s1">ERROR: Chunk failed at level:4, tier_type:0</span></p>
<p class="gmail-p1"><span class="gmail-s1">FAILED CONTIG:6537645</span></p>
<p class="gmail-p2"><span class="gmail-s1"></span><br></p>
<p class="gmail-p1"><span class="gmail-s1">ERROR: Chunk failed at level:4, tier_type:0</span></p>
<p class="gmail-p1"><span class="gmail-s1">FAILED CONTIG:6537659</span></p>
<p class="gmail-p2"><span class="gmail-s1"></span><br></p></div></div><div class="gmail_extra"><br><div class="gmail_quote">On Thu, Mar 2, 2017 at 1:25 PM, Carson Holt <span dir="ltr"><<a href="mailto:carsonhh@gmail.com" target="_blank">carsonhh@gmail.com</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div style="word-wrap:break-word">Try reinstalling blast, or upgrade to a newer version of blast.<div><br></div><div>—Carson</div><div><br></div><div><br><div><blockquote type="cite"><div><div class="h5"><div>On Mar 2, 2017, at 1:05 PM, Marcus Naymik <<a href="mailto:mnaymik@tgen.org" target="_blank">mnaymik@tgen.org</a>> wrote:</div><br class="m_-3896368484793307179Apple-interchange-newline"></div></div><div><div><div class="h5"><div dir="ltr"><div>
<br class="m_-3896368484793307179webkit-block-placeholder"></div><p class="m_-3896368484793307179gmail-p1"><span class="m_-3896368484793307179gmail-s1">I have maker running with MPI and I get this error over and over again for every contig. Any Ideas?</span></p><p class="m_-3896368484793307179gmail-p1"><span class="m_-3896368484793307179gmail-s1"><br></span></p><p class="m_-3896368484793307179gmail-p1"><span class="m_-3896368484793307179gmail-s1">MAKER WARNING: All old files will be erased before continuing</span></p><p class="m_-3896368484793307179gmail-p1"><span class="m_-3896368484793307179gmail-s1">#-----------------------------<wbr>------------------------------<wbr>----------</span></p><p class="m_-3896368484793307179gmail-p1"><span class="m_-3896368484793307179gmail-s1">Now starting the contig!!</span></p><p class="m_-3896368484793307179gmail-p1"><span class="m_-3896368484793307179gmail-s1">SeqID: 5239</span></p><p class="m_-3896368484793307179gmail-p1"><span class="m_-3896368484793307179gmail-s1">Length: 1395</span></p><p class="m_-3896368484793307179gmail-p1"><span class="m_-3896368484793307179gmail-s1">#-----------------------------<wbr>------------------------------<wbr>----------</span></p><p class="m_-3896368484793307179gmail-p2"><span class="m_-3896368484793307179gmail-s1"></span><br></p><p class="m_-3896368484793307179gmail-p2"><span class="m_-3896368484793307179gmail-s1"></span><br></p><p class="m_-3896368484793307179gmail-p1"><span class="m_-3896368484793307179gmail-s1">Error: NCBI C++ Exception:</span></p><p class="m_-3896368484793307179gmail-p1"><span class="m_-3896368484793307179gmail-s1"> "/packages/BUILDS/ncbi-blast-<wbr>2.2.28+-src/c++/src/corelib/<wbr>ncbiobj.cpp", line 925: Criti</span></p><div>
<br class="m_-3896368484793307179webkit-block-placeholder"></div><p class="m_-3896368484793307179gmail-p2"><span class="m_-3896368484793307179gmail-s1"></span><br></p></div>
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