<div dir="ltr"><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff">Hi Maker folks,</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff"><br></div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff">I have several issues with a plant genome annotation that I am currently doing but perhaps the most recurrent issues are:</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff"><br></div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff">1/ CDSs that are missed where significant rna-seq evidence is there (figure artemis01)</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff">2/ vice versa where one or two exons are added without rna-seq evidence/intron hints (figure artemis02)<br></div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff"><br></div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff">info about the runs:</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff">1/ using augustus with a pre-existing model for a related plant that has high homology to the one I am annotating</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff">2/ umask=1 (seems to do better than umask=0; is this a good thing to do)</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff">3/ evm = 1 (seems to perform better than emv=0)</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff">4/ repeatmasking (denovo + repbase)</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff"><br></div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff">Best,</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff">/SB</div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff"><br></div><div class="gmail_default" style="font-family:trebuchet ms,sans-serif;font-size:small;color:#9900ff"><br></div><div><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><pre style="color:rgb(0,0,0);font-size:13px;word-wrap:break-word;white-space:pre-wrap"><font face="trebuchet ms, sans-serif"><font size="1">___________________________________________</font><font size="1"><font size="1">____________</font>
</font><font size="1">Salim Bougouffa<sup>(PhD)</sup>, Postdoctoral Fellow
4700 KAUST, CBRC, Blg<sup>3</sup>. Office<sup>4326-WS05</sup>,<br>Thuwal, Jeddah, KSA, 23955-6900<br>(966) 012 808 2963 || <a href="mailto:salim.bougouff@kaust.edu.sa" target="_blank">salim.bougouff@kaust.edu.sa</a></font></font></pre></div></div></div></div></div>
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