<html dir="ltr">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
<style type="text/css" id="owaParaStyle"></style>
</head>
<body fpstyle="1" ocsi="0">
<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;">Hi there,
<div><br>
</div>
<div>I am attempting to submit a fungal genome to NCBI and have run into quite a few errors running tbl2asn. I know this isn't directly related to MAKER, but I'm hoping that someone here has been through this process and would be able to give some insight
(or at least point me in the direction of another knowledgeable source)!</div>
<div><br>
</div>
<div>Here is a general overview of the process that have used so far:</div>
<div>1. Converted MAKER GFF3 files to tbl files using GAG (ran options remove_introns_shorter_than 10 and fix_start_stop and added functional annotation as well). This seems to work well to convert the GFF3 to tbl.</div>
<div>2. Use tbl2asn to convert the tbl to sqn file. This also seems to work, but I am getting lots of errors in the .val output file, which I am unsure how to address. There are...</div>
<div><br>
</div>
<div>
<div> 56 ERROR: SEQ_FEAT.BadTrailingHyphen</div>
<div> 1525 ERROR: SEQ_FEAT.InternalStop</div>
<div> 1142 ERROR: SEQ_FEAT.NoStop</div>
<div> 10 ERROR: SEQ_FEAT.PartialProblem</div>
<div> 2368 ERROR: SEQ_FEAT.StartCodon</div>
<div> 2368 ERROR: SEQ_INST.BadProteinStart</div>
<div> 1525 ERROR: SEQ_INST.StopInProtein</div>
<div> 8821 WARNING: SEQ_FEAT.NotSpliceConsensusAcceptor</div>
<div> 8543 WARNING: SEQ_FEAT.NotSpliceConsensusDonor</div>
<div> 83 WARNING: SEQ_FEAT.PartialProblem</div>
<div> 1 WARNING: SEQ_FEAT.ProteinNameEndsInBracket</div>
<div> 19 WARNING: SEQ_FEAT.ShortExon</div>
<div> 270 INFO: SEQ_FEAT.RareSpliceConsensusDonor</div>
</div>
<div><br>
</div>
<div>Also, just as a side note, has anyone tried the new table2asn_GFF converter that is up to convert GFF3 directly to sqn? I was thinking that I would give that a shot hoping that it would help with some of these errors. However, I was instantly met with
an error as well. <span style="font-size: 13.3333px;">"Too many positional arguments (1), the offending value: ends."</span></div>
<div><span style="font-size: 13.3333px;"><br>
</span></div>
<div><span style="font-size: 13.3333px;">Thank you so much in advance for any help you are able to give!</span></div>
<div><span style="font-size: 13.3333px;"><br>
</span></div>
<div><span style="font-size: 13.3333px;">Glenna</span></div>
</div>
</body>
</html>