<div dir="ltr"><br><div class="gmail_quote">---------- Forwarded message ----------<br>From: <b class="gmail_sendername">Isabel Marcelino</b> <span dir="ltr"><<a href="mailto:isabel.marcelino.im@gmail.com">isabel.marcelino.im@gmail.com</a>></span><br>Date: 23 August 2017 at 11:37<br>Subject: Errors with Blast engine and Fasta index<br>To: <a href="mailto:maker-devel@googlegroups.com">maker-devel@googlegroups.com</a><br><br><br><div dir="ltr">Hello,<br><br>I am new to linux and bioinformatics but I managed to set MAKER (v2.31.9) running smoothly a few weeks ago and I could perform a de novo annotation of one of my genome. In the meantime, I worked on other topics and, yesterday, when I tried to annotate another genome, MAKER was not working. <br>The following message was appearing:<br><br>BLAST engine error: Warning: Sequence contains no data <br>deleted:0 hits<br>stop here: NODE_1_length_465927_cov_94.<wbr>9908<br>ERROR: Fasta index error<br>at /home/administrateur/results/<wbr>Isa/ANNOTATION/MAKER_running_<wbr>tools/RUN_MAKER/bin/../lib/<wbr>Process/MpiChunk.pm line 1095.<br>        Process::MpiChunk::__ANON__() called at /home/administrateur/results/<wbr>Isa/ANNOTATION/MAKER_running_<wbr>tools/RUN_MAKER/bin/../lib/<wbr>Error.pm line 415<br>        eval {...} called at /home/administrateur/results/<wbr>Isa/ANNOTATION/MAKER_running_<wbr>tools/RUN_MAKER/bin/../lib/<wbr>Error.pm line 407<br>        Error::subs::try('CODE(<wbr>0x4cd5320)', 'HASH(0x4cd53f8)') called at /home/administrateur/results/<wbr>Isa/ANNOTATION/MAKER_running_<wbr>tools/RUN_MAKER/bin/../lib/<wbr>Process/MpiChunk.pm line 4224<br>        Process::MpiChunk::_go('<wbr>Process::MpiChunk=HASH(<wbr>0x4cfae20)', 'run', 'HASH(0x4cca458)', 3, 0) called at /home/administrateur/results/<wbr>Isa/ANNOTATION/MAKER_running_<wbr>tools/RUN_MAKER/bin/../lib/<wbr>Process/MpiChunk.pm line 341<br>        Process::MpiChunk::run('<wbr>Process::MpiChunk=HASH(<wbr>0x4cfae20)', 2) called at ../../bin/maker line 1614<br>        main::run('ARRAY(0x4af3e68)', 2) called at ../../bin/maker line 999<br>--> rank=2, hostname=SRV-WGS<br>ERROR: Failed while preparing masked sequence<br>ERROR: Chunk failed at level:3, tier_type:0<br>FAILED CONTIG:NODE_1_length_465927_<wbr>cov_94.9908<br><br>I tried to solve the problem (re-install Repeatmasker and blast, check location of the several executables, check tmp folder, re-install MAKER, etc) but still, I am not able to solve the problem.<br>Could you please help me out with this? Thank you so much.<br><br>Cheers,<br>Isabel <br><br>******************************<wbr>*******************<br>Isabel MARCELINO, PhD<br>Unité Environnement Santé<br>Institut Pasteur de Guadeloupe<br>Morne Jolivière - B.P. 484<br>97183 LES ABYMES Cedex<br>Guadeloupe, FWI<br><br></div>
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