<div dir="ltr">Hello,<div><br></div><div>Good day,</div><div><br></div><div>I am trying to assign putative gene function to the maker generated fasta. I am using NCBI</div><div><br></div><div>I keep getting this error</div><div>  <span style="font-family:Menlo;font-size:11px">Command line argument error: Argument "query". File is not accessible:</span><span style="font-family:Menlo;font-size:11px">  </span><span style="font-family:Menlo;font-size:11px">`muc1_genome_snap2.all.maker.snap_masked.proteins.fasta'</span></div><div><span style="font-family:Menlo;font-size:11px"><br></span></div><div><span style="font-family:Menlo;font-size:11px">What do I do?</span></div><div><span style="font-family:Menlo;font-size:11px"><br></span></div><div><font face="Menlo"><span style="font-size:11px">can I use blast2go in place of ncbi command line software?</span></font></div></div><div class="gmail_extra"><br clear="all"><div><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr">Nnadi Nnaemeka Emmanuel<div>Department of Microbiology,</div><div>Faculty of Natural and Applied Science,</div><div>Plateau State University, Bokkos, Plateau State, Nigeria.</div><div>Publications:  <a href="https://www.researchgate.net/profile/Emmanuel_Nnadi/publications" target="_blank">https://www.researchgate.net/profile/Emmanuel_Nnadi/publications</a></div></div></div></div>
<br><div class="gmail_quote">On Mon, Aug 28, 2017 at 2:00 PM, Ence,daniel <span dir="ltr"><<a href="mailto:d.ence@ufl.edu" target="_blank">d.ence@ufl.edu</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">



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Hi Emmanuel, In order for anyone to help you, you need post to the mailing list the command and output (including errors) of the step that didn’t work. 
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<div>Thanks,</div>
<div>Daniel Ence</div>
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<div>On Aug 27, 2017, at 10:16 AM, Emmanuel Nnadi <<a href="mailto:eennadi@gmail.com" target="_blank">eennadi@gmail.com</a>> wrote:</div>
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<div dir="auto">Hello all,
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<div dir="auto">I downloaded Maker and tried to install it. I succeeded in installing all prerequisites however running maker ./build install, it showed that maker installed.</div>
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<div dir="auto">However trying to run maker it wouldn't run.</div>
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<div dir="auto">Please how do I install maker to run on local computer?</div>
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<div dir="auto">Thanks<br>
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<div data-smartmail="gmail_signature" dir="auto">Nnadi Nnaemeka Emmanuel<br>
Department of Microbiology,<br>
Faculty of Natural and Applied Science,<br>
Plateau State University, Bokkos, Plateau State, Nigeria.<br>
Publications:  <a href="https://www.researchgate.net/profile/Emmanuel_Nnadi/publications" target="_blank">https://www.researchgate.net/<wbr>profile/Emmanuel_Nnadi/<wbr>publications</a><br>
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