<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">I’m not sure what you mean by NCBI. Do you mean BLAST? If so, you probably did not format and index your input database before running BLAST. See BLAST documentation.<div class=""><br class=""></div><div class="">Also the file you are using —> <span style="font-family: Menlo; font-size: 11px;" class="">muc1_genome_snap2.all.maker.snap_masked.proteins.fasta</span></div><div class=""><span style="font-size: 11px;" class=""><br class=""></span></div><div class="">That is not the maker result file. That is a reference fasta of raw SNAP results. The MAKER result file will have a name like this (see maker documentation) —> muc1_genome_snap2.all.maker.proteins.fasta</div><div class=""><br class=""></div><div class="">—Carson</div><div class=""><br class=""><div class=""><br class=""></div><div class=""><br class=""><div><blockquote type="cite" class=""><div class="">On Sep 24, 2017, at 3:24 PM, Emmanuel Nnadi <<a href="mailto:eennadi@gmail.com" class="">eennadi@gmail.com</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div dir="ltr" class="">Hello,<div class=""><br class=""></div><div class="">Good day,</div><div class=""><br class=""></div><div class="">I am trying to assign putative gene function to the maker generated fasta. I am using NCBI</div><div class=""><br class=""></div><div class="">I keep getting this error</div><div class=""> <span style="font-family:Menlo;font-size:11px" class="">Command line argument error: Argument "query". File is not accessible:</span><span style="font-family:Menlo;font-size:11px" class=""> </span><span style="font-family:Menlo;font-size:11px" class="">`muc1_genome_snap2.all.maker.snap_masked.proteins.fasta'</span></div><div class=""><span style="font-family:Menlo;font-size:11px" class=""><br class=""></span></div><div class=""><span style="font-family:Menlo;font-size:11px" class="">What do I do?</span></div><div class=""><span style="font-family:Menlo;font-size:11px" class=""><br class=""></span></div><div class=""><font face="Menlo" class=""><span style="font-size:11px" class="">can I use blast2go in place of ncbi command line software?</span></font></div></div><div class="gmail_extra"><br clear="all" class=""><div class=""><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr" class="">Nnadi Nnaemeka Emmanuel<div class="">Department of Microbiology,</div><div class="">Faculty of Natural and Applied Science,</div><div class="">Plateau State University, Bokkos, Plateau State, Nigeria.</div><div class="">Publications: <a href="https://www.researchgate.net/profile/Emmanuel_Nnadi/publications" target="_blank" class="">https://www.researchgate.net/profile/Emmanuel_Nnadi/publications</a></div></div></div></div>
<br class=""><div class="gmail_quote">On Mon, Aug 28, 2017 at 2:00 PM, Ence,daniel <span dir="ltr" class=""><<a href="mailto:d.ence@ufl.edu" target="_blank" class="">d.ence@ufl.edu</a>></span> wrote:<br class=""><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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Hi Emmanuel, In order for anyone to help you, you need post to the mailing list the command and output (including errors) of the step that didn’t work.
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<div class="">Thanks,</div>
<div class="">Daniel Ence</div>
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<div class="">On Aug 27, 2017, at 10:16 AM, Emmanuel Nnadi <<a href="mailto:eennadi@gmail.com" target="_blank" class="">eennadi@gmail.com</a>> wrote:</div>
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<div dir="auto" class="">Hello all,
<div dir="auto" class=""><br class="">
</div>
<div dir="auto" class="">I downloaded Maker and tried to install it. I succeeded in installing all prerequisites however running maker ./build install, it showed that maker installed.</div>
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<div dir="auto" class="">However trying to run maker it wouldn't run.</div>
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<div dir="auto" class="">Please how do I install maker to run on local computer?</div>
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<div dir="auto" class="">Thanks<br class="">
<br class="">
<div data-smartmail="gmail_signature" dir="auto" class="">Nnadi Nnaemeka Emmanuel<br class="">
Department of Microbiology,<br class="">
Faculty of Natural and Applied Science,<br class="">
Plateau State University, Bokkos, Plateau State, Nigeria.<br class="">
Publications: <a href="https://www.researchgate.net/profile/Emmanuel_Nnadi/publications" target="_blank" class="">https://www.researchgate.net/<wbr class="">profile/Emmanuel_Nnadi/<wbr class="">publications</a><br class="">
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