<div dir="ltr"><div><div><div><div><div>Hi there,<br></div>Did you perform quality and adapter trimming of your raw reads? That's actually an assembly issue.<br></div>I would seriously encourage you to redo the assembly before continuing.</div><div><br></div>If that isnt possible, start by removing those sequences and split the contigs at those places as suggested in the report.<br><br>For the annotation part, not 100% sure but I'd say start with the "Merge/resolve legacy annotations" steps but maybe Carson or Daniel have a different suggestion<br><a href="http://weatherby.genetics.utah.edu/MAKER/wiki/index.php/MAKER_Tutorial_for_GMOD_Online_Training_2014#Merge.2FResolve_Legacy_Annotations">http://weatherby.genetics.utah.edu/MAKER/wiki/index.php/MAKER_Tutorial_for_GMOD_Online_Training_2014#Merge.2FResolve_Legacy_Annotations</a><br><br></div>Cheers,<br></div>Xabi<br><div><div><br></div></div></div><div class="gmail_extra"><br><div class="gmail_quote">On 24 October 2017 at 00:30, Emmanuel Nnadi <span dir="ltr"><<a href="mailto:eennadi@gmail.com" target="_blank">eennadi@gmail.com</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr">Hello<br><div><br></div><div>Good day.</div><div><br></div><div>Please I submitted my sequence to NCBI and they sent back this contamination report.</div><div><br></div><div>Please how do I use maker to effect the correction</div><div><br></div><div><br></div><div><div><div class="m_7461417095194769316gmail_signature" data-smartmail="gmail_signature"><div dir="ltr">Nnadi Nnaemeka Emmanuel<div>Department of Microbiology,</div><div>Faculty of Natural and Applied Science,</div><div>Plateau State University, Bokkos, Plateau State, Nigeria.</div><div>Publications:  <a href="https://www.researchgate.net/profile/Emmanuel_Nnadi/publications" target="_blank">https://www.researchgate.net/<wbr>profile/Emmanuel_Nnadi/<wbr>publications</a></div></div></div></div>
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<br></blockquote></div><br><br clear="all"><br>-- <br><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div>Xabier Vázquez-Campos, <i>PhD</i><br><i>Research Associate</i><br>NSW Systems Biology Initiative<br>School of Biotechnology and Biomolecular Sciences<br>
The University of New South Wales<br>Sydney NSW 2052 AUSTRALIA<br></div></div></div></div></div></div></div></div></div></div></div>
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