<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi Emmanuel, I recommend looking into what Carson suggested. If you edited the fasta files for the “NNN” characters for the transcripts or reference genome and then resubmitted without changing the gff3 coordinates, then that would result in these kind of errors. <div class=""><br class=""></div><div class="">~Daniel</div><div class=""><br class=""></div><div class=""><br class=""><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><br class=""><div><blockquote type="cite" class=""><div class="">On Nov 2, 2017, at 5:02 PM, Emmanuel Nnadi <<a href="mailto:eennadi@gmail.com" class="">eennadi@gmail.com</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><a href="https://drive.google.com/file/d/1rPSI3HhceRuJMzWO9hAej-BzMNUSKz7g/view?usp=drive_web" target="_blank" style="font-family: arial; font-size: 13px; font-style: normal; font-variant: normal; font-weight: bold; letter-spacing: normal; line-height: 13px; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(245, 245, 245); display: inline-block; max-width: 366px; overflow: hidden; text-overflow: ellipsis; white-space: nowrap; text-decoration: none; padding: 1px 0px; border: none;" class=""><img src="https://ssl.gstatic.com/docs/doclist/images/icon_10_generic_list.png" style="vertical-align: bottom; border: none;" class=""> <span dir="ltr" style="color: rgb(17, 85, 204); text-decoration: none; vertical-align: bottom;" class="">muc_functional.blast.gff</span></a></div></blockquote></div><br class=""></div></div></body></html>