<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi Lahcen,<div class=""><br class=""></div><div class="">Nothing comes right to mind for what could be causing this error. If you want to compress your FASTA and send it to me I can try and recreate the error and try and debug it.</div><div class=""><br class=""></div><div class="">Thanks,</div><div class="">Mike<br class=""><div><blockquote type="cite" class=""><div class="">On Nov 14, 2017, at 7:15 AM, lahcen campbell <<a href="mailto:lahcencampbell@gmail.com" class="">lahcencampbell@gmail.com</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div dir="ltr" class="">Hi MAKER community,<div class=""><br class=""></div><div class="">I was hoping someone could help me. I have a very unusual error with two different versions of maker I have tested so far. This error shouldn't be happening but it occurs time and again no matter what I try. I have tried using 2.31.6_mpich3_icc and 2.31_mpich3</div><div class=""><br class=""></div><div class="">Note that version 2.31.6_mpich3_icc is one I have used countless times and produced final MAKER annotations without issue. So its not that this version has issues to date. </div><div class=""><br class=""></div><div class="">Basically, this is a brand new MAKER analysis, I am only trying to train SNAP in this first round. I am following the MakerTutorial as documented this time around and I can't get past the initial SNAP train stage. </div><div class=""><br class=""></div><div class="">I have a single genome file with, 10 Long scaffolds making up just under 11MB (subsampled from my original full length assembly) of sequence data in which to train SNAP. The fasta file is not corrupted, and has been generated in various ways in order to test formatting issues etc. </div><div class=""><br class=""></div><div class="">I have only edited the maker_opts file and changed:<br class=""></div><div class=""><br class=""></div><div class=""><b class="">genome=</b></div><div class=""><b class="">protein=</b></div><div class=""><b class="">protein2genome=1</b></div><div class=""><br class=""></div><div class="">But see attached my maker CTL files. </div><div class=""><br class=""></div><div class="">The error consistently returned to me:</div><div class=""><br class=""></div><div class=""><div class=""><b class=""><i class="">Skipping the contig because it is too short!!</i></b></div><div class=""><b class=""><i class="">SeqID: contig_WHATEVER</i></b></div><div class=""><b class=""><i class="">Length: 0</i></b></div></div><div class=""><br class=""></div><div class=""><i class="">The sequences are no where near too short. This was verified independently outside maker to be sure. </i></div><div class=""><i class=""><br class=""></i></div><div class=""><i class="">The headers are as follows:</i></div><div class=""><div class=""><br class=""></div><div class="">>tig00000458 len=2889428 reads=4143 covStat=1793.77 gappedBases=no class=contig suggestRepeat=no suggestCircular=no</div><div class="">>tig00000159 len=3515005 reads=5100 covStat=2143.94 gappedBases=no class=contig suggestRepeat=no suggestCircular=no</div><div class="">>tig00006117 len=1009519 reads=1168 covStat=804.93 gappedBases=no class=contig suggestRepeat=no suggestCircular=no</div><div class="">>tig00000419 len=2633986 reads=3938 covStat=1519.93 gappedBases=no class=contig suggestRepeat=no suggestCircular=no</div><div class="">>tig00027677 len=108573 reads=86 covStat=86.05 gappedBases=no class=contig suggestRepeat=no suggestCircular=no</div><div class="">>tig00021790 len=202251 reads=158 covStat=184.12 gappedBases=no class=contig suggestRepeat=no suggestCircular=no</div><div class="">>tig00316948 len=280333 reads=237 covStat=253.23 gappedBases=no class=contig suggestRepeat=no suggestCircular=no</div><div class="">>tig00019606 len=149709 reads=82 covStat=150.02 gappedBases=no class=contig suggestRepeat=no suggestCircular=no</div><div class="">>tig00023852 len=189461 reads=115 covStat=192.28 gappedBases=no class=contig suggestRepeat=no suggestCircular=no</div><div class="">>tig00316994 len=19742 reads=1 covStat=0.00 gappedBases=no class=contig suggestRepeat=no suggestCircular=no</div><div class=""><br class=""></div><div class="">I have just about given up, I have no idea why its happening it makes zero sense. </div><div class=""><br class=""></div><div class="">Any help or information as to why this might be happening would be amazing. </div><div class=""><br class=""></div><div class="">Thank you in advance. </div><div class="">Lahcen</div><div class=""><br class=""></div>-- <br class=""><div class="gmail_signature"><div dir="ltr" class=""><div class=""><div dir="ltr" class=""><div class=""><div dir="ltr" class=""><div class=""><div dir="ltr" class="">==========================================<br class="">> Dr. Lahcen Campbell <<div class="">> Contact: <a href="mailto:lahcencampbell@gmail.com" target="_blank" class="">lahcencampbell@gmail.com</a> <</div><div class="">> <a href="https://www.ebi.ac.uk/about/people/lahcen-campbell" target="_blank" class="">https://www.ebi.ac.uk/about/people/lahcen-campbell</a> <</div><div class=""><div class=""><div class="">==========================================</div></div></div></div></div></div></div></div></div></div></div>
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