<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">The datasets do not look too large. The failure you are seeing is happening outside of MAKER. So there is something wrong on the system itself. You will probably have to reinstall perl against your local libraries especially if you reinstalled BerkleyDB. Or try downloading the latest stable release of Perl (it comes precompiled against static libraries - Berkeley DB version 1.x which can help avoid some issues). You will have to reinstall MAKER to use that version of Perl (MAKER uses the perl version used to call Build.PL during the install).<div class=""><br class=""></div><div class="">If you are running on something like FreeBSD, it may just break Perl’s DB_File.</div><div class=""><br class=""></div><div class="">Also this note from CPAN —></div><div class="">Although DB_File is intended to be used with Berkeley DB version 1, it can also be used with version 2, 3 or 4. In this case the interface is limited to the functionality provided by Berkeley DB 1.x.</div><div class=""><br class=""></div><div class="">If reinstalling tools does not work around your issue, you may just have to run on a different system.</div><div class=""><br class=""></div><div class="">—Carson</div><div class=""><br class=""><div><br class=""><blockquote type="cite" class=""><div class="">On Apr 15, 2018, at 8:34 AM, ohon Kin <<a href="mailto:ohon.kin@gmail.com" class="">ohon.kin@gmail.com</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div dir="ltr" class="">
<span class=""></span><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><br class=""></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><br class=""></div><p class="gmail-p1" style="margin:0px;font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.9)">
<span class=""></span>
</p><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">grep -c ">" Ca_kacst.fna<span class="gmail-Apple-converted-space"> </span></span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">32572</span></div>
<br class=""><div class=""><br class="webkit-block-placeholder"></div><div style="margin: 0px; font-variant-numeric: normal; font-variant-east-asian: normal; font-stretch: normal; line-height: normal; background-color: rgba(0, 0, 0, 0.901961);" class=""><font color="#28fe14" face="Andale Mono" class=""><span style="font-size:17px;font-variant-ligatures:no-common-ligatures" class="">the EST i have are assembled to contigs </span></font></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span style="font-variant-ligatures:no-common-ligatures" class="">grep -c ">" Ca_EST</span><span class="gmail-Apple-converted-space" style="font-variant-ligatures:no-common-ligatures"> </span><br class=""></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">23602</span></div>
<br class=""><div class=""><br class=""></div><div class="">
<span class=""></span><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">grep -c ">" Ca__protein.faa<span class="gmail-Apple-converted-space"> </span></span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">26729</span></div>
<br class=""></div><div class="">these are my input-data i have reinstall perl as your instructions please have a look, the tool still 1T not enough will stop while running of the run</div><div class=""><br class=""></div><div class="">i get this Error</div><div class="">
<span class=""></span><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">ad$ ./maker</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">STATUS: Parsing control files...</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">WARNING: 'max_dna_len' is set too low.<span class="gmail-Apple-converted-space"> </span>The minimum value permited is 50,000.</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">max_dna_len will be reset to 50,000</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961); min-height: 19px;" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures"></span><br class=""></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">STATUS: Processing and indexing input FASTA files...</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="gmail-Apple-converted-space"> </span>Increase page size</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="gmail-Apple-converted-space"> </span>Increase page size</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="gmail-Apple-converted-space"> </span>Increase page size</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="gmail-Apple-converted-space"> </span>Increase page size</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="gmail-Apple-converted-space"> </span>Increase page size</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="gmail-Apple-converted-space"> </span>Increase page size</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="gmail-Apple-converted-space"> </span>Increase page size</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="gmail-Apple-converted-space"> </span>Increase page size</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="gmail-Apple-converted-space"> </span>Increase page size</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="gmail-Apple-converted-space"> </span>Increase page size</span></div><div style="margin: 0px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; font-stretch: normal; font-size: 17px; line-height: normal; font-family: "Andale Mono"; color: rgb(40, 254, 20); background-color: rgba(0, 0, 0, 0.901961);" class=""><span class="gmail-s1" style="font-variant-ligatures:no-common-ligatures">Filesize limit exceeded: 25</span></div>
<br class=""></div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><font size="4" class=""><b class=""><u class="">my maker_opt</u></b></font></div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><div class="">#-----Genome (these are always required)</div><div class="">genome=/Users/mohanad/Documents/maker/data/Ca_dromedarius_kacst.fna #genome sequence (fasta file or fasta embeded in GFF3 file)</div><div class="">organism_type=eukaryotic #eukaryotic or prokaryotic. Default is eukaryotic</div><div class=""><br class=""></div><div class="">#-----Re-annotation Using MAKER Derived GFF3</div><div class="">maker_gff= #MAKER derived GFF3 file</div><div class="">est_pass=0 #use ESTs in maker_gff: 1 = yes, 0 = no</div><div class="">altest_pass=0 #use alternate organism ESTs in maker_gff: 1 = yes, 0 = no</div><div class="">protein_pass=0 #use protein alignments in maker_gff: 1 = yes, 0 = no</div><div class="">rm_pass=0 #use repeats in maker_gff: 1 = yes, 0 = no</div><div class="">model_pass=0 #use gene models in maker_gff: 1 = yes, 0 = no</div><div class="">pred_pass=0 #use ab-initio predictions in maker_gff: 1 = yes, 0 = no</div><div class="">other_pass=0 #passthrough anyything else in maker_gff: 1 = yes, 0 = no</div><div class=""><br class=""></div><div class="">#-----EST Evidence (for best results provide a file for at least one)</div><div class="">est=/Users/mohanad/Documents/maker/data/Ca_dromedarius_EST #set of ESTs or assembled mRNA-seq in fasta format</div><div class="">altest= #EST/cDNA sequence file in fasta format from an alternate organism</div><div class="">est_gff= #aligned ESTs or mRNA-seq from an external GFF3 file</div><div class="">altest_gff= #aligned ESTs from a closly relate species in GFF3 format</div><div class=""><br class=""></div><div class="">#-----Protein Homology Evidence (for best results provide a file for at least one)</div><div class="">protein=/Users/mohanad/Documents/maker/data/Ca_dromedarius_V1.0_protein.faa #protein sequence file in fasta format (i.e. from mutiple oransisms)</div><div class="">protein_gff= #aligned protein homology evidence from an external GFF3 file</div><div class=""><br class=""></div><div class="">#-----Repeat Masking (leave values blank to skip repeat masking)</div><div class="">model_org=all #select a model organism for RepBase masking in RepeatMasker</div><div class="">rmlib= #provide an organism specific repeat library in fasta format for RepeatMasker</div><div class="">repeat_protein= #provide a fasta file of transposable element proteins for RepeatRunner</div><div class="">rm_gff= #pre-identified repeat elements from an external GFF3 file</div><div class="">prok_rm=0 #forces MAKER to repeatmask prokaryotes (no reason to change this), 1 = yes, 0 = no</div><div class="">softmask=1 #use soft-masking rather than hard-masking in BLAST (i.e. seg and dust filtering)</div><div class=""><br class=""></div><div class="">#-----Gene Prediction</div><div class="">snaphmm= #SNAP HMM file</div><div class="">gmhmm= #GeneMark HMM file</div><div class="">augustus_species= #Augustus gene prediction species model</div><div class="">fgenesh_par_file= #FGENESH parameter file</div><div class="">pred_gff= #ab-initio predictions from an external GFF3 file</div><div class="">model_gff= #annotated gene models from an external GFF3 file (annotation pass-through)</div><div class="">est2genome=1#infer gene predictions directly from ESTs, 1 = yes, 0 = no</div><div class="">protein2genome=0 #infer predictions from protein homology, 1 = yes, 0 = no</div><div class="">trna=0 #find tRNAs with tRNAscan, 1 = yes, 0 = no</div><div class="">snoscan_rrna= #rRNA file to have Snoscan find snoRNAs</div><div class="">unmask=0 #also run ab-initio prediction programs on unmasked sequence, 1 = yes, 0 = no</div><div class=""><br class=""></div><div class="">#-----Other Annotation Feature Types (features MAKER doesn't recognize)</div><div class="">other_gff= #extra features to pass-through to final MAKER generated GFF3 file</div><div class=""><br class=""></div><div class="">#-----External Application Behavior Options</div><div class="">alt_peptide=C #amino acid used to replace non-standard amino acids in BLAST databases</div><div class="">cpus=1 #max number of cpus to use in BLAST and RepeatMasker (not for MPI, leave 1 when using MPI)</div><div class=""><br class=""></div><div class="">#-----MAKER Behavior Options</div><div class="">max_dna_len=10000 #length for dividing up contigs into chunks (increases/decreases memory usage)</div><div class="">min_contig=1 #skip genome contigs below this length (under 10kb are often useless)</div><div class=""><br class=""></div><div class="">pred_flank=200 #flank for extending evidence clusters sent to gene predictors</div><div class="">pred_stats=0 #report AED and QI statistics for all predictions as well as models</div><div class="">AED_threshold=1 #Maximum Annotation Edit Distance allowed (bound by 0 and 1)</div><div class="">min_protein=0 #require at least this many amino acids in predicted proteins</div><div class="">alt_splice=0 #Take extra steps to try and find alternative splicing, 1 = yes, 0 = no</div><div class="">always_complete=0 #extra steps to force start and stop codons, 1 = yes, 0 = no</div><div class="">map_forward=0 #map names and attributes forward from old GFF3 genes, 1 = yes, 0 = no</div><div class="">keep_preds=0 #Concordance threshold to add unsupported gene prediction (bound by 0 and 1)</div><div class=""><br class=""></div><div class="">split_hit=10000 #length for the splitting of hits (expected max intron size for evidence alignments)</div><div class="">single_exon=0 #consider single exon EST evidence when generating annotations, 1 = yes, 0 = no</div><div class="">single_length=250 #min length required for single exon ESTs if 'single_exon is enabled'</div><div class="">correct_est_fusion=0 #limits use of ESTs in annotation to avoid fusion genes</div><div class=""><br class=""></div><div class="">tries=2 #number of times to try a contig if there is a failure for some reason</div><div class="">clean_try=0 #remove all data from previous run before retrying, 1 = yes, 0 = no</div><div class="">clean_up=0 #removes theVoid directory with individual analysis files, 1 = yes, 0 = no</div><div class="">TMP= #specify a directory other than the system default temporary directory for temporary files</div></div><div class=""><br class=""></div></div><div class="gmail_extra"><br class=""><div class="gmail_quote">On 11 April 2018 at 20:57, Carson Holt <span dir="ltr" class=""><<a href="mailto:carsonhh@gmail.com" target="_blank" class="">carsonhh@gmail.com</a>></span> wrote:<br class=""><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div style="word-wrap:break-word;line-break:after-white-space" class="">The issue is with Berkley DB. BioPerl is using perl’s DB_File module to index the fastas. <div class=""><div class=""><br class=""></div><div class="">1. Make sure you do not have an extremely large number of reads in the fasta files (i.e. mRNA-seq data which cannot be used directly as input to MAKER, you must assemble it first into transcriptome contigs)</div><div class="">2. Reinstall perl and compile against the newly installed BerkleyDB libraries.</div><div class="">3. Remove the brew installed BerkleyDB and use perl’s precompiled DB_File module.</div><div class=""><br class=""></div><div class="">You can count reads in your fasta input using this command (replace file.fasta)</div><div class=""><br class=""></div><div class="">grep -c “>” file.fasta</div><div class=""><br class=""></div><div class="">If your counts are really high (i.e. higher than a few hundred thousand maximum), then you have a data issue. You are either giving too much data or the wrong data as input.</div><div class=""><br class=""></div><div class="">—Carson</div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><br class=""><blockquote type="cite" class=""><div class="">On Apr 11, 2018, at 11:39 AM, ohon Kin <<a href="mailto:ohon.kin@gmail.com" target="_blank" class="">ohon.kin@gmail.com</a>> wrote:</div><br class="m_-5736245261331413232Apple-interchange-newline"><div class=""><br class="m_-5736245261331413232Apple-interchange-newline"><span style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;float:none;display:inline!important" class="">hello ; Carson </span><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class="">i really would appreciate your help im kind of having same issue </div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class="">i get this Error when i run maker i assumed that it required big memory space </div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><div style="margin:0px;font-style:normal;font-variant-caps:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.901961)" class=""><span class="m_-5736245261331413232s1" style="font-variant-ligatures:no-common-ligatures">STATUS: Processing and indexing input FASTA files...</span></div><div style="margin:0px;font-style:normal;font-variant-caps:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.901961)" class=""><span class="m_-5736245261331413232s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="m_-5736245261331413232Apple-converted-space"> <span class="m_-5736245261331413232Apple-converted-space"> </span></span>Increase page size</span></div><div style="margin:0px;font-style:normal;font-variant-caps:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.901961)" class=""><span class="m_-5736245261331413232s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="m_-5736245261331413232Apple-converted-space"> <span class="m_-5736245261331413232Apple-converted-space"> </span></span>Increase page size</span></div><div style="margin:0px;font-style:normal;font-variant-caps:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.901961)" class=""><span class="m_-5736245261331413232s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="m_-5736245261331413232Apple-converted-space"> <span class="m_-5736245261331413232Apple-converted-space"> </span></span>Increase page size</span></div><div style="margin:0px;font-style:normal;font-variant-caps:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.901961)" class=""><span class="m_-5736245261331413232s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="m_-5736245261331413232Apple-converted-space"> <span class="m_-5736245261331413232Apple-converted-space"> </span></span>Increase page size</span></div><div style="margin:0px;font-style:normal;font-variant-caps:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.901961)" class=""><span class="m_-5736245261331413232s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="m_-5736245261331413232Apple-converted-space"> <span class="m_-5736245261331413232Apple-converted-space"> </span></span>Increase page size</span></div><div style="margin:0px;font-style:normal;font-variant-caps:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.901961)" class=""><span class="m_-5736245261331413232s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="m_-5736245261331413232Apple-converted-space"> <span class="m_-5736245261331413232Apple-converted-space"> </span></span>Increase page size</span></div><div style="margin:0px;font-style:normal;font-variant-caps:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.901961)" class=""><span class="m_-5736245261331413232s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="m_-5736245261331413232Apple-converted-space"> <span class="m_-5736245261331413232Apple-converted-space"> </span></span>Increase page size</span></div><div style="margin:0px;font-style:normal;font-variant-caps:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.901961)" class=""><span class="m_-5736245261331413232s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="m_-5736245261331413232Apple-converted-space"> <span class="m_-5736245261331413232Apple-converted-space"> </span></span>Increase page size</span></div><div style="margin:0px;font-style:normal;font-variant-caps:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.901961)" class=""><span class="m_-5736245261331413232s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="m_-5736245261331413232Apple-converted-space"> <span class="m_-5736245261331413232Apple-converted-space"> </span></span>Increase page size</span></div><div style="margin:0px;font-style:normal;font-variant-caps:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.901961)" class=""><span class="m_-5736245261331413232s1" style="font-variant-ligatures:no-common-ligatures">HASH: Out of overflow pages.<span class="m_-5736245261331413232Apple-converted-space"> <span class="m_-5736245261331413232Apple-converted-space"> </span></span>Increase page size</span></div><div style="margin:0px;font-style:normal;font-variant-caps:normal;font-weight:normal;font-stretch:normal;font-size:17px;line-height:normal;font-family:"Andale Mono";color:rgb(40,254,20);background-color:rgba(0,0,0,0.901961)" class=""><span class="m_-5736245261331413232s1" style="font-variant-ligatures:no-common-ligatures">Filesize limit exceeded: 25</span></div><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class="">while working 1T of my Hard-disc capacity seems not enough for maker annotation</div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class="">i think something wrong in my input data or the dependencies </div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""> would you please advice on the matter and elaborate solutions please </div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class="">i have install BerkleyDB using brew </div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class="">The input giving to Maker as followed :</div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class="">Genome , EST , Protein. all in Fasta format, downloaded from NCBI ---> then added it directly to maker for annotation</div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""> do i have to apply these data pre-process before it applied to maker </div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class=""><br class=""></div><div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none" class="">On Thursday, 7 December 2017 19:00:52 UTC+3, Carson Holt wrote:<blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"><div style="word-wrap:break-word;line-break:after-white-space" class="">The FASTA file gets indexed by BioPerl using BerkleyDB.<span class="m_-5736245261331413232Apple-converted-space"> </span></div></blockquote><div class=""> </div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"><div style="word-wrap:break-word;line-break:after-white-space" class="">I’m guessing there is something odd about your input file and the database has run out of HASHes for indexing.<span class="m_-5736245261331413232Apple-converted-space"> </span></div></blockquote><div class=""> </div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"><div style="word-wrap:break-word;line-break:after-white-space" class="">You can google if there is a setting you can configure in BerkleyDB on Mac.</div></blockquote><div class=""> </div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"><div style="word-wrap:break-word;line-break:after-white-space" class="">But I suspect you are doing something like giving the raw reads from an mRNA-seq experiment or DNA sequencing to MAKER (resulting in billions of entrires to be indexed), which would be incorrect. MAKER can’t handle raw data. You must first assemble it using using like Trinity for example for mRNA.<div class=""><br class=""></div><div class="">Thanks,</div><div class="">Carson<br class=""><div class=""><br class=""><blockquote type="cite" class=""><div class="">On Dec 7, 2017, at 8:53 AM, Scott Cain <<a rel="nofollow" class="">sc...@</a><a href="http://scottcain.net/" target="_blank" class="">scottcain.net</a>> wrote:</div><br class=""><div class=""><div dir="ltr" class="">Hi Guinara,<div class=""><br class=""></div><div class="">I don't know (though my guess would be that you're running out of memory). I'm cc'ing the MAKER developer's mailing list to see if anybody on that list knows.</div><div class=""><br class=""></div><div class="">Scott</div><div class=""><br class=""></div></div><div class=""><br class=""><div class="gmail_quote">On Wed, Dec 6, 2017 at 8:36 PM, Gulnara Tagirdzhanova<span class="m_-5736245261331413232Apple-converted-space"> </span><span dir="ltr" class=""><<a rel="nofollow" class="">tagi...@</a><a href="http://ualberta.ca/" target="_blank" class="">ualbert<wbr class="">a.ca</a>></span><span class="m_-5736245261331413232Apple-converted-space"> </span>wrote:<br class=""><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"><div dir="ltr" class="">Hello,<div class=""><br class=""></div><div class="">I got this error running maker on mac:</div><div class=""><br class=""></div><div class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">STATUS: Parsing control files...</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">STATUS: Processing and indexing input FASTA files...</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">HASH: Out of overflow pages. Increase page size</span><br style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class="">Filesize limit exceeded: 25</span></div><div class=""><font face="-apple-system, BlinkMacSystemFont, Roboto, Open Sans, Helvetica Neue, sans-serif" class=""><br class=""></font></div><div class=""><span style="font-family:-apple-system,BlinkMacSystemFont,Roboto,"Open Sans","Helvetica Neue",sans-serif;font-size:13px" class=""></span><font face="-apple-system, BlinkMacSystemFont, Roboto, Open Sans, Helvetica Neue, sans-serif" class="">Is there anything that could solve it?</font></div><div class=""><font face="-apple-system, BlinkMacSystemFont, Roboto, Open Sans, Helvetica Neue, sans-serif" class=""><br class=""></font></div><div class=""><font face="-apple-system, BlinkMacSystemFont, Roboto, Open Sans, Helvetica Neue, sans-serif" class="">Thank you,</font></div><div class=""><font face="-apple-system, BlinkMacSystemFont, Roboto, Open Sans, Helvetica Neue, sans-serif" class="">Gulnara<br class=""></font><div class=""><br class=""></div><div class=""><br class=""></div></div></div></blockquote></div><br class=""><br clear="all" class=""><span class="HOEnZb"><font color="#888888" class=""><div class=""><br class=""></div>--<span class="m_-5736245261331413232Apple-converted-space"> </span><br class=""><div class="">------------------------------<wbr class="">------------------------------<wbr class="">------------<br class="">Scott Cain, Ph. D. scott at scottcain dot net<br class="">GMOD Coordinator (<a href="http://gmod.org/" rel="nofollow" target="_blank" class="">http://gmod.org/</a>) 216-392-3087<br class="">Ontario Institute for Cancer Research</div></font></span></div><span class="HOEnZb"><font color="#888888" class="">______________________________<wbr class="">_________________<br class="">maker-devel mailing list<br class=""><a rel="nofollow" class="">maker...@</a><a href="http://box290.bluehost.com/" target="_blank" class="">box290.bluehost.</a><a href="http://box290.bluehost.com/" target="_blank" class="">com</a><br class=""><a href="http://box290.bluehost.com/mailman/listinfo/maker-devel_yandell-lab.org" rel="nofollow" target="_blank" class="">http://box290.bluehost.com/mai<wbr class="">lman/listinfo/maker-devel_yand<wbr class="">ell-lab.org</a></font></span></div></blockquote></div></div></div></blockquote></div></div></blockquote></div><br class=""></div></div></blockquote></div><br class=""><br clear="all" class=""><div class=""><br class=""></div>-- <br class=""><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr" class=""><table border="1" bgcolor="#FAFAFF" align="center" width="100%" style="font-size:12.8px" class=""><tbody class=""><tr class=""><td width="50%" align="righ" valign="middle" class=""><div style="margin: 0in 0in 0.0001pt; font-size: 9pt; font-family: Arial, sans-serif; text-align: justify;" class=""><span dir="LTR" class=""></span><strong class="">Warning: </strong>This message and its attachment, if any, are confidential and may contain information protected by law. If you are not the intended recipient, please contact the sender immediately and delete the message and its attachment, if any. You should not copy the message and its attachment, if any, or disclose its contents to any other person or use it for any purpose. Statements and opinions expressed in this e-mail and its attachment, if any, are those of the sender, and do not necessarily reflect those of kacst. accepts no liability for any damage caused by this email.</div></td><td width="50%" align="right" class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif; text-align: justify; direction: rtl;" class=""><span lang="AR-SA" style="font-size:9pt" class=""><strong class="">تحذير:</strong></span><span dir="LTR" class=""></span><span lang="AR-SA" dir="LTR" style="font-size:9pt" class=""><span dir="LTR" class=""></span> </span><span lang="AR-SA" style="font-size:9pt" class="">هذه الرسالة وما تحويه من مرفقات (إن وجدت) تمثل وثيقة سرية قد تحتوي على معلومات محمية بموجب القانون. إذا لم تكن الشخص المعني بهذه الرسالة فيجب عليك تنبيه المُرسل بخطأ وصولها إليك، وحذف الرسالة ومرفقاتها (إن وجدت)، ولا يجوز لك نسخ أو توزيع هذه الرسالة أو مرفقاتها (إن وجدت) أو أي جزء منها، أو البوح بمحتوياتها للغير أو استعمالها لأي غرض. علماً بأن فحوى هذه الرسالة ومرفقاتها (ان وجدت) تعبر عن رأي المُرسل وليس بالضرورة رأي مدينة الملك عبدالعزيز، ولا تتحمل المدينة أي مسئولية عن الأضرار الناتجة عن ما قد يحتويه هذا البريد</span><span dir="LTR" class=""></span><span dir="LTR" style="font-size:9pt" class=""><span dir="LTR" class=""></span>.</span></div></td></tr></tbody></table></div></div>
</div>
</div></blockquote></div><br class=""></div></body></html>