<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
<style type="text/css" style="display:none;"><!-- P {margin-top:0;margin-bottom:0;} --></style>
</head>
<body dir="ltr">
<div id="divtagdefaultwrapper" style="font-size: 12pt; color: rgb(0, 0, 0); font-family: Calibri, Helvetica, sans-serif, Helvetica, EmojiFont, "Apple Color Emoji", "Segoe UI Emoji", NotoColorEmoji, "Segoe UI Symbol", "Android Emoji", EmojiSymbols;" dir="ltr">
<p style="margin-top:0;margin-bottom:0">Hello, </p>
<p style="margin-top:0;margin-bottom:0"><br>
</p>
<p style="margin-top:0;margin-bottom:0">Maker successfully annotated the genome but keeps failing for one contig with error message attached below. Can you please help me troubleshoot this ? There is no issue with augustus for rest of the contigs. </p>
<p style="margin-top:0;margin-bottom:0"><br>
</p>
<p style="margin-top:0;margin-bottom:0">Thank you</p>
<p style="margin-top:0;margin-bottom:0"><br>
</p>
<p style="margin-top:0;margin-bottom:0">Aditi </p>
<p style="margin-top:0;margin-bottom:0"><br>
</p>
<p style="margin-top:0;margin-bottom:0"></p>
<div>#--------- command -------------#</div>
<div>Widget::augustus:</div>
<div>~/augustus-3.2/bin/augustus --species=Cxxx --strand=forward --UTR=off --hintsfile=/tmp/maker_dSn0ps/0/71_0.26253049-26257472.Cxxx.auto_annotator.xdef.augustus --extrinsicCfgFile=/data/home/srs57/programs/augustus-3.2/config/extrinsic/extrinsic.MPE.cfg
/tmp/maker_dSn0ps/0/71_0.26253049-26257472.Cxxx.auto_annotator.augustus.fasta > /tmp/maker_dSn0ps/0/71_0.26253049-26257472.Cxxx.auto_annotator.augustus</div>
<div>#-------------------------------#</div>
<div>Sampling error in intron model. state=14 base=4422</div>
<div><br>
</div>
<div>~/augustus-3.2/bin/augustus: ERROR</div>
<div><span style="white-space:pre"></span>Tried to sample from empty list.</div>
<div><br>
</div>
<div>Sampling error in intron model. state=14 base=4422</div>
<div><span style="font-size: 12pt;">~/augustus-3.2/bin/augustus: ERROR</span><br>
</div>
<div><span style="white-space:pre"></span>Tried to sample from empty list.</div>
<div><br>
</div>
<div>ERROR: Augustus failed</div>
<div>--> rank=NA, hostname=xxx</div>
<div>ERROR: Failed while annotating transcripts</div>
<div>ERROR: Chunk failed at level:1, tier_type:4</div>
<div>FAILED CONTIG:Contigxxx</div>
<div><br>
</div>
<div>ERROR: Chunk failed at level:6, tier_type:0</div>
<div>FAILED CONTIG:<span style="font-family: Calibri, Helvetica, sans-serif, Helvetica, EmojiFont, "Apple Color Emoji", "Segoe UI Emoji", NotoColorEmoji, "Segoe UI Symbol", "Android Emoji", EmojiSymbols; font-size: 16px;">Contigxxx</span></div>
<div><br>
</div>
<div>examining contents of the fasta file and run log</div>
<div><br>
</div>
<br>
<p></p>
</div>
</body>
</html>