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<p class="MsoNormal">Thanks,<o:p></o:p></p>
<p class="MsoNormal">Do you have metrics on how MAKER performs on annotating a single chromosome on a single machine? For example, will I see anything close to 16X speed-up using a 16 core machine, and does performance improvement saturate at a certain number
of cores?<o:p></o:p></p>
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<p class="MsoNormal">-Keith<o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black">Carson Holt <carsonhh@gmail.com><br>
<b>Date: </b>Monday, February 4, 2019 at 12:33 PM<br>
<b>To: </b>"DECKER, KEITH F [AG/1005]" <keith.decker@bayer.com><br>
<b>Cc: </b>"maker-devel@yandell-lab.org" <maker-devel@yandell-lab.org><br>
<b>Subject: </b>Re: [maker-devel] MAKER on AWS<o:p></o:p></span></p>
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<p class="MsoNormal">You can try and stand up a cluster inside AWS, or like you said just start independent instances each with their own piece of the total dataset. There is a tools called fasta_tool inside of maker that makes it easy to split up the dataset
into equal sized chunks. <o:p></o:p></p>
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<p class="MsoNormal">Alternatively, CyVerse has set up an interesting MAKER wrapper (WQ-MAKER) that launches multiple cloud instances for MAKER and handles data chunking for you (they’ve been using XSEDE cloud resources through the NSF) —><o:p></o:p></p>
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<p class="MsoNormal"><a href="http://ccl.cse.nd.edu/research/papers/maker-service-ic2e2018.pdf">http://ccl.cse.nd.edu/research/papers/maker-service-ic2e2018.pdf</a><o:p></o:p></p>
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<p class="MsoNormal">Here is an example of an external project using their setup —> <a href="http://onsnetwork.org/kubu4/2018/08/07/genome-annotation-olympia-oyster-genome-using-wq-maker-instance-on-jetstream/">http://onsnetwork.org/kubu4/2018/08/07/genome-annotation-olympia-oyster-genome-using-wq-maker-instance-on-jetstream/</a><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">—Carson<o:p></o:p></p>
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<p class="MsoNormal">On Feb 4, 2019, at 11:09 AM, DECKER, KEITH F [AG/1005] <<a href="mailto:keith.decker@bayer.com">keith.decker@bayer.com</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal">I would like to evaluate the use of MAKER on AWS, but I am unsure what the best approach to parallelization would be.<span style="font-size:12.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal">I found this old post on STARCLUSTER,<span class="apple-converted-space"> </span><a href="http://efish.integrativebiology.msu.edu/2015/02/10/annotate.html"><span style="color:#954F72">http://efish.integrativebiology.msu.edu/2015/02/10/annotate.html</span></a><span style="font-size:12.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal">but my understanding is that STARCLUSTER and its successors (cfncluster and parallel cluster) can be challenging to set up and use. <span style="font-size:12.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal"> <span style="font-size:12.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal">So my questions are<span style="font-size:12.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal"> <span style="font-size:12.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal">1. Has anyone had recent success running MAKER on cfncluster or parallel cluster in AWS?<span style="font-size:12.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal">2. Would it be reasonable to just split up N chromosomes across N ECS instances and collect the results at the end? If so, does it make sense to run each chromosome level annotation on for example an m4.16xlarge instance with 64 cores
and 256 GB of RAM? Or is there a maximum number of cores at which the benefits from parallelization saturate?<span style="font-size:12.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal"> <span style="font-size:12.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal">Thanks and sorry for the long question<span style="font-size:12.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal">Keith<span style="font-size:12.0pt"><o:p></o:p></span></p>
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