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<div><span></span>Dear MAKER developers,</div><div><span style="font-family: "" microsoft="" yahei="" ui'";="" font-size:="" 14px;="" color:="" rgb(0,="" 0,="" 0);="" background-color:="" rgba(0,="" font-weight:="" normal;="" font-style:="" normal;text-decoration:="" none;'=""> </span><span style="font-family: ''; font-size: 10.5pt; line-height: 1.5; background-color: window;"> I recently use the MAKER to do gene annotation. But even I use the gff3 file as the EST evidence, the result shows no gene that marked by est2genome (I do use the parameter </span><span style="font-family: ''; font-size: 10.5pt; line-height: 1.5; background-color: window;">est2genome=1</span><span style="background-color: window; font-family: ''; font-size: 10.5pt; line-height: 1.5;">). It may means that the gff3 seems doesn't work. So what's the problem? How can I solve it? Eager for you assistance.</span></div><div><span style="font-family: "" microsoft="" yahei="" ui'";="" font-size:="" 14px;="" color:="" rgb(0,="" 0,="" 0);="" background-color:="" rgb(255,="" 255,="" 255);="" font-weight:="" normal;="" font-style:="" normal;text-decoration:="" none;'=""> </span><span style="font-family: ''; font-size: 10.5pt; line-height: 1.5; background-color: window;"> </span><span style="font-size: 10.5pt; line-height: 1.5; background-color: window;">Faithfully yours.</span></div><div><span style="font-family: "" microsoft="" yahei="" ui'";="" font-size:="" 14px;="" color:="" rgb(0,="" 0,="" 0);="" background-color:="" rgb(255,="" 255,="" 255);="" font-weight:="" normal;="" font-style:="" normal;text-decoration:="" none;'=""> </span><span style="font-family: ''; font-size: 10.5pt; line-height: 1.5; background-color: window;"> Yucheng Liu</span></div>
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<div><span><b>Yucheng Liu</b><div><span style="font-family: "" microsoft="" yahei="" ui'";="" font-size:="" 14px;="" color:="" rgb(0,="" 0,="" 0);="" background-color:="" rgba(0,="" font-weight:="" normal;="" font-style:="" normal;text-decoration:="" none;'="">Institute of Genetics and Developmental Biology, CAS</span></div><div><span style="font-family: "" microsoft="" yahei="" ui'";="" font-size:="" 14px;="" color:="" rgb(0,="" 0,="" 0);="" background-color:="" rgba(0,="" font-weight:="" normal;="" font-style:="" normal;text-decoration:="" none;'="">Beijing, 100101 China</span></div><div><span style="font-family: "" microsoft="" yahei="" ui'";="" font-size:="" 14px;="" color:="" rgb(0,="" 0,="" 0);="" background-color:="" rgba(0,="" font-weight:="" normal;="" font-style:="" normal;text-decoration:="" none;'="">Tel: 86-010-64801362</span></div><div><span style="font-family: "" microsoft="" yahei="" ui'";="" font-size:="" 14px;="" color:="" rgb(0,="" 0,="" 0);="" background-color:="" rgba(0,="" font-weight:="" normal;="" font-style:="" normal;text-decoration:="" none;'="">E-mail: ychliu@genetics.ac.cn</span></div></span></div>
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