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Use version 1.3.1 (last stable version for 1.X series), we haven’t updated maker to handle tRNAscan version 2 options and output yet.
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<div class="">All version here —> <a href="http://trna.ucsc.edu/software/" class="">http://trna.ucsc.edu/software/</a></div>
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<div class="">—Carson</div>
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<div class="">On May 22, 2019, at 10:18 AM, 李青 Li, Qing <<a href="mailto:liqing850104@gmail.com" class="">liqing850104@gmail.com</a>> wrote:</div>
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<div dir="ltr" class="">Hi Nazila,
<div class="">The right one to contact is Carson and Maker developer group (cced).</div>
<div class="">best,</div>
<div class="">Qing </div>
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<div dir="ltr" class="gmail_attr">On Wed, May 22, 2019 at 6:39 AM Koochekian, Nazila <<a href="mailto:koochen@miamioh.edu" class="">koochen@miamioh.edu</a>> wrote:<br class="">
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<div class="">Dear Maker group</div>
<div class="">My name is Nazila, I am a Ph.D. student at Miami University. I'm working on <i class="">Phrynosoma platyrhinos </i>genome annotation and I'm trying to use MAKER. So as a requirement I'm trying to use tRNAscan. First I used the latest version (2.0)
and I got the first -q file but I faced a warning like this:</div>
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<div dir="ltr" class="">WARNING: -q exists already.</div>
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<div dir="ltr" class=""> (O)verwrite file, (A)ppend to file, or (Q)uit program? </div>
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<div class="">And I tried overwrite and append but it just froze and didn't work anymore.</div>
<div class="">So I tried to use an older version (1.3.1) but this one is hard to make it work too. <span style="background-color:transparent" class="">I'm getting an error:</span></div>
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<div class="">Can't locate object method "new" via package "tRNAscanSE::Constants" (perhaps you forgot to load "tRNAscanSE::Constants"?) at /home/koochen/tRNAscan-SE-1.3.1/tRNAscan-SE line 57.</div>
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<div class="">Would you please help me with it? Which version should I use? And how could I solve the problem for that version?</div>
<div class="">Looking forward for your reply;</div>
<div class="">Thanks,</div>
<div class="">Nazila</div>
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