<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Make sure your fast file is not compressed (i.e. .gz or .bz extension). Otherwise one of the entries in the middle of the file likely has nonsense characters. Also you can delete the mpi_blastdb under the *.maker.output directory to force it top rebuild any indexes.<div class=""><br class=""></div><div class="">—Carson</div><div class=""><br class=""><div class=""><br class=""></div><div class=""><div><br class=""><blockquote type="cite" class=""><div class="">On Jan 31, 2020, at 2:50 PM, Emily Abernathy <<a href="mailto:eabernathy@ucdavis.edu" class="">eabernathy@ucdavis.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div dir="ltr" class="">Hello,<div class=""> I am running MAKER for the first time and I have been unable to resolve an error. The error is as follows:</div><div class=""><div class=""><span id="cid:ii_k62p45340"><image.png></span><br class=""></div><div class="">I am using a genome that I assembled in Supernova v2 with headers that resemble this:</div><div class="">>1 edges=1057764..867844 left=488686 right=145511 ver=1.10 style=3<br class=""></div><div class=""><br class=""></div><div class="">and I downloaded two fasta files from ENSEMBL whose headers resemble this:</div><div class="">>ENSTGUT00000018018.1 cdna chromosome:taeGut3.2.4:8_random:2849599:2959678:-1 gene:ENSTGUG00000017338.1 gene_biotype:protein_coding transcript_biotype:protein_coding<br class=""></div><div class=""><br class=""></div><div class="">and</div><div class=""><br class=""></div><div class="">>ENSTGUP00000017615.1 pep chromosome:taeGut3.2.4:23_random:205321:209117:1 gene:ENSTGUG00000017337.1 transcript:ENSTGUT00000018017.1 gene_biotype:protein_coding transcript_biotype:protein_coding<br class=""></div><div class=""><br class=""></div><div class="">These are my only input FASTA files and I have been struggling to fix this error for almost a month now. Any and all advice on how to fix this error is much appreciated!</div><div class=""><br class=""></div><div class="">Thanks in advance,</div><div class="">E. Abernathy</div><div class=""> </div><div class=""><br class=""></div><div class=""><br class=""></div>-- <br class=""><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr" class=""><span style="font-size:12.8px" class="">Emily Abernathy</span><div style="font-size:12.8px" class="">Graduate Group in Ecology<br class=""><div class="">University of California, Davis</div><div class=""><a href="http://hulllabucd.wix.com/hulllab" target="_blank" class="">http://hulllabucd.wix.com/hulllab</a></div></div></div></div></div></div>
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