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--></style></head><body lang=EN-GB link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal>Hi all,<o:p></o:p></p><p class=MsoNormal>I am using the “maker_functional_gff” script to update my gff3 by adding functional annotation from blast against uniprot, however I do get the following error when running the code:<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Can't use string ("") as a HASH ref while "strict refs" in use at /opt/maker/bin/maker_functional_gff line 55, <$IN> line 277933.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>I checked the line and it appears like the other lines in the gff3 file so I was wondering if you guys know what is causing the error?<o:p></o:p></p><p class=MsoNormal>The “offending” line and the following one are: <o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>tig00632243 maker gene 2926 22617 . - . ID=Gacu_00045928;Name=Gacu_00045928;Alias=maker-tig00632243-snap-gene-0.9;Dbxref=MobiDBLite:mobidb-lite,PANTHER:PTHR15288,PANTHER:PTHR15288:SF5;<o:p></o:p></p><p class=MsoNormal>tig00632243 maker mRNA 2926 22617 . - . ID=Gacu_00045928-RA;Parent=Gacu_00045928;Name=Gacu_00045928-RA;Alias=maker-tig00632243-snap-gene-0.9-mRNA-1;_AED=0.08;_QI=0|0|0|0.75|0.81|0.83|12|0|714;_eAED=0.34;Dbxref=MobiDBLite:mobidb-lite,PANTHER:PTHR15288,PANTHER:PTHR15288:SF5;<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Thanks<o:p></o:p></p><p class=MsoNormal>Luca<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>