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Hello maker community,</div>
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I am annotating a fish genome with Maker 2.31.10, for which I have transcript evidence and proteins from closely related species. I am running maker in several passes and pass the gff output to the nest pass every time here (
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<div style="margin: 0px">"#-----Re-annotation Using MAKER Derived GFF3<br>
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<div style="margin: 0px">maker_gff= #re-annotate genome based on this gff3 file",</div>
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), is this wrong? and where is the difference running the maker from scratch without re-supplying every time the new gff?</div>
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Secondly, in the second round after predictors training, have I to keep <span>est2genome=1 , </span><span>protein2genome=1, ests and proteins evidence or not?</span></div>
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<span>How do we switch all thesre parameters? </span></div>
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Any input from experienced maker users is welcome<br>
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Thank you for your help</div>
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<i>Θεόδωρος Δανης </i></div>
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<span style="font-family:calibri,helvetica,sans-serif; font-size:12pt; color:rgb(0,0,0); background-color:rgba(0,0,0,0)"><i style="">Thodoris Danis</i></span></div>
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