<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">If you can create a two column file (column1 being the old ID and column2 being the new ID), you can use the map_fasta_ids and map_gff_ids to rename everything with a corresponding line in the two column file. Make sure no ID's occur twice (in old or new), it will create issues. The maker ID will not be lost, instead it will move to the Alias= field in the GFF3.<div class=""><br class=""></div><div class="">—Carson</div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><div><br class=""><blockquote type="cite" class=""><div class="">On Oct 5, 2020, at 7:47 AM, Zoe Clarke <<a href="mailto:zoe.clarke@utoronto.ca" class="">zoe.clarke@utoronto.ca</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class="">Thanks Carson!</div><div style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class=""><br class=""></div><div style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class="">I managed to get the GFF3 file fixed and Maker is now recognizing it the way it should.</div><div style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class=""><br class=""></div><div style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class="">I am hoping I can also ask for your advice on functional annotation with Maker. The result of my current run will be gff and fasta files that have the original gff3 genes plus Maker's. The original gff3 file was annotated with HGNC gene symbols, so these new files are a mix of gene symbols and Maker-derived gene names. I would ideally like my final annotation to consist almost entirely of gene symbols. Is the only way to do this (after using Blastp and InterProScan to get putative homology) to undergo an additional manual curation process through something like Apollo?</div><div style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class=""><br class=""></div><div style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class="">Thanks again,</div><div style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class="">Zoe</div><div style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class=""><div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class=""><br class=""></div><div id="Signature" class=""><div class=""><div class=""></div><div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class=""><span style="font-size: 10pt;" class="">______________________________________</span></div><div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class=""><span style="font-size: 10pt;" class="">Zoe Clarke</span></div><div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class=""><span style="font-size: 10pt;" class="">PhD candidate in Computational Biology at U of T</span></div><div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class=""><span style="font-size: 10pt;" class="">Lab profile: </span><a href="http://baderlab.org/Zoe%20Clarke" class=""><span style="font-size: 10pt;" class="">http://baderlab.org/Zoe%20Clarke</span></a></div><div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;" class=""><span style="font-size: 10pt;" class="">Personal website: </span><a href="https://zoe-clarke.weebly.com/" class=""><span style="font-size: 10pt;" class="">https://zoe-clarke.weebly.com/</span></a></div></div></div></div><div id="appendonsend" style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class=""></div><hr tabindex="-1" style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; display: inline-block; width: 1571.90625px;" class=""><span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;" class=""></span><div id="divRplyFwdMsg" dir="ltr" style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class=""><font face="Calibri, sans-serif" style="font-size: 11pt;" class=""><b class="">From:</b><span class="Apple-converted-space"> </span>Carson Holt <<a href="mailto:carsonhh@gmail.com" class="">carsonhh@gmail.com</a>><br class=""><b class="">Sent:</b><span class="Apple-converted-space"> </span>October 2, 2020 4:50 PM<br class=""><b class="">To:</b><span class="Apple-converted-space"> </span>Zoe Clarke <<a href="mailto:zoe.clarke@utoronto.ca" class="">zoe.clarke@utoronto.ca</a>><br class=""><b class="">Cc:</b><span class="Apple-converted-space"> </span><a href="mailto:maker-devel@yandell-lab.org" class="">maker-devel@yandell-lab.org</a><span class="Apple-converted-space"> </span><<a href="mailto:maker-devel@yandell-lab.org" class="">maker-devel@yandell-lab.org</a>><br class=""><b class="">Subject:</b><span class="Apple-converted-space"> </span>Re: [maker-devel] map_forward and temporary storage questions</font><div class=""> </div></div><div class="" style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; word-wrap: break-word; line-break: after-white-space;"><div style="background-color: rgb(37, 53, 90);" class=""><div style="font-size: 10pt; font-family: sans-serif; color: white; font-style: normal; font-weight: bold; padding: 0.2em;" class=""><span style="color: rgb(255, 165, 0);" class="">EXTERNAL EMAIL: </span><span class="Apple-converted-space"> </span>Treat content with extra caution. </div></div><div class="">MAKER uses GFF3 format which is not the same as GTF. You will need to convert your file to GFF3 format.<div class=""><br class=""></div><div class="">You can try this online tool (I haven’t used it to tell you how well it works) <a href="http://www.sequenceontology.org/cgi-bin/converter.cgi" class="">http://www.sequenceontology.org/cgi-bin/converter.cgi</a> </div><div class=""><br class=""></div><div class="">There are also a number of other resources available when you google "how to convert GTF to GFF3”.</div><div class=""><br class=""></div><div class="">—Carson</div><div class=""><br class=""><div class=""><br class=""><blockquote type="cite" class=""><div class="">On Sep 25, 2020, at 3:17 AM, Zoe Clarke <<a href="mailto:zoe.clarke@utoronto.ca" class="">zoe.clarke@utoronto.ca</a>> wrote:</div><br class="x_Apple-interchange-newline"><div class=""><div class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><div class="" style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;">Hello!</div><div class="" style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;"><br class=""></div><div class="" style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;">I am currently running Maker on a 2.5GB genome that has already had a list of ~8000 genes very thoroughly annotated. My hope is to find and annotate the rest of the genes using ESTs and protein homology. However, I tested Maker on a single contig of my genome (there are ~20,000 contigs) and I can't find any of the genes from my original gtf file even though I followed all of the instructions in this wiki: <a href="http://weatherby.genetics.utah.edu/MAKER/wiki/index.php/Updating_annotations_in_light_of_new_data" id="LPlnk" class="">http://weatherby.genetics.utah.edu/MAKER/wiki/index.php/Updating_annotations_in_light_of_new_data</a> (I entered the original gff under model_gff, and used map_forward=1). I am worried this is because my gff3 file isn't formatted properly. Here are a few lines in my gff file as an example:</div><div class="" style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;">--------------------------------------------</div><div class="" style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;"><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 transcript 1094446 1105585 . + . ID=DIMT1.1;geneID=DIMT1</span><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 exon 1094446 1094521 97.75 + . Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 exon 1094874 1094947 97.75 + . Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 exon 1095459 1095545 97.75 + . Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 exon 1097351 1097412 97.75 + . Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 exon 1097492 1097585 97.75 + . Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 exon 1097670 1097719 97.75 + . Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 exon 1098957 1099080 97.75 + . Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 exon 1099217 1099309 97.75 + . Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 exon 1100870 1100934 97.75 + . Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 exon 1101967 1102030 97.75 + . Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 exon 1103784 1103890 97.75 + . Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 exon 1105543 1105585 97.75 + . Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 CDS 1094446 1094521 . + 0 Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 CDS 1094874 1094947 . + 2 Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 CDS 1095459 1095545 . + 0 Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 CDS 1097351 1097412 . + 0 Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 CDS 1097492 1097585 . + 1 Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 CDS 1097670 1097719 . + 0 Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 CDS 1098957 1099080 . + 1 Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 CDS 1099217 1099309 . + 0 Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 CDS 1100870 1100934 . + 0 Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 CDS 1101967 1102030 . + 1 Parent=DIMT1.1</span></div><div class=""><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 CDS 1103784 1103890 . + 0 Parent=DIMT1.1</span></div><span class="" style="font-size: 10pt;">WCK01_AAF20200214_F8-ctg36 ovaltine_v0.13 CDS 1105543 1105582 . + 1 Parent=DIMT1.1</span><br class=""></div><div class="x__EReadonly_1 x__EType_OWALinkPreview x__Entity x__EId_OWALinkPreview"></div>--------------------------------------</div><div class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><span class="" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;">This is from the contig I used as a test for maker, and I can't find DIMT1.1 in the final gff file. At first I thought it might be because "geneID" is a listed attribute, but changing this to "Name" didn't help. Do you have any ideas why these genes might not be mapping forward? If it's something I can fix in the gff file, I am hoping I can fix it and use it for the second round of Maker after I have trained Snap.</span><br class=""></div><div class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><span class="" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;"><br class=""></span></div><div class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><span class="" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;">Also, do you think a better quality annotation would results from Snap trained from this curated list of ~8000 genes (that has been expertly done) or by the round 1 output of Maker?</span></div><div class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><span class="" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;"><br class=""></span></div><div class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><span class="" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;">A final question: I am having memory storage issues with Maker, as it is currently taking up ~15TB of storage with temporary files. <span class="" style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(255, 255, 255); display: inline !important;">I am running Maker on a cluster and whenever my submitted Maker job runs out of memory it fails, so I have to resubmit it about every hour, which leaves a lot of temporary folders<span class="x_Apple-converted-space"> </span><span class="" style="font-family: Calibri, Helvetica, sans-serif; background-color: rgb(255, 255, 255); display: inline !important;">(e.g. maker_x6V2y4)</span> in my directory.</span> I notice that some of these temporary files haven't been updated in days - is it okay to delete them?</span></div><div class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><span class="" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;"><br class=""></span></div><div class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><span class="" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;">Thank you so much for your help!</span></div><div class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><span class="" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;">Zoe</span></div><div class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><div id="x_Signature" class=""><div class=""><div class=""></div><div class="" style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;"><span class="" style="font-size: 10pt;">______________________________________</span></div><div class="" style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;"><span class="" style="font-size: 10pt;">Zoe Clarke</span></div><div class="" style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;"><span class="" style="font-size: 10pt;">PhD candidate in Computational Biology at U of T</span></div><div class="" style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;"><span class="" style="font-size: 10pt;">Lab profile: </span><a href="http://baderlab.org/Zoe%20Clarke" class=""><span class="" style="font-size: 10pt;">http://baderlab.org/Zoe%20Clarke</span></a></div><div class="" style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;"><span class="" style="font-size: 10pt;">Personal website: </span><a href="https://zoe-clarke.weebly.com/" class=""><span class="" style="font-size: 10pt;">https://zoe-clarke.weebly.com/</span></a></div></div></div></div><span class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none; float: none; display: inline !important;">_______________________________________________</span><br class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><span class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none; float: none; display: inline !important;">maker-devel mailing list</span><br class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><a href="mailto:maker-devel@yandell-lab.org" class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px;">maker-devel@yandell-lab.org</a><br class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; text-decoration: none;"><a href="http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org" class="" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px;">http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org</a></div></blockquote></div></div></div></div></div></blockquote></div><br class=""></div></body></html>