<div dir="ltr"><div>Hi Kevin,</div><div><br></div><div>I have checked the 2 files.</div><div>About
<a href="http://genomes.ua.edu/Kocot/2021-09-10_Maker/pasa_predictions.gff3" id="gmail-m_-3511602195170912938LPlnk460561" target="_blank">pasa_predictions.gff3</a>:</div><div>It is parsed without any problem with
<a href="http://agat_convert_sp_gxf2gxf.pl" target="_blank">agat_convert_sp_gxf2gxf.pl</a> in about 10 mins. MAKER (>=v3) should be able to use it like that, but you might prefer to convert it into match/match_part style using the
<a href="http://agat_sp_alignment_output_style.pl" target="_blank">agat_sp_alignment_output_style.p</a>l. The
match/match_part style works in all of MAKER version.<br></div><div>
About
<a href="http://genomes.ua.edu/Kocot/2021-09-10_Maker/protein_alignments.gff3" id="gmail-m_-3511602195170912938LPlnk159063" target="_blank">protein_alignments.gff3</a>: <br></div><div>This file is more problematic, it contains only 1 feature type, which is level1 in AGAT (i.e. like a gene), and in the current state is expecting sub-features. On top of that, the ID attribute is confusing because it is supposed to be unique, and it is not. So here the commands you should launch to get a proper file for MAKER:</div><div>First<br></div><div>```</div><div>sed 's/nucleotide_to_protein_match/match_part/' protein_alignments.gff3 | sed 's/ID=/Parent=/' > protein_alignments_repared.gff3</div><div>```</div><div>Then</div><div>```</div><div><a href="http://agat_convert_sp_gxf2gxf.pl">agat_convert_sp_gxf2gxf.pl</a> --gff
protein_alignments_repared.gff3 -o
protein_alignments_clean.gff3 </div><div>```</div><div>Then you should be good with a proper match/match_part file.</div><div><br></div><div>Best,</div><div><br></div><div>/Jacques<br></div><div><br></div><div class="gmail_quote"><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="auto"><blockquote type="cite"><div dir="ltr">
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
Hi Carson and Jacques,</div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
<br>
</div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
Thank you both very much for the help. It looks like my PASA and exonerate (via Funannotate) gff3 files are not correctly formatted for maker. I've uploaded them here just in case it might be helpful for you to see them, but I will try to figure out how to
reformat them correctly following Jacques's advice.</div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
<a href="http://genomes.ua.edu/Kocot/2021-09-10_Maker/pasa_predictions.gff3" id="gmail-m_-3511602195170912938LPlnk460561" target="_blank">http://genomes.ua.edu/Kocot/2021-09-10_Maker/pasa_predictions.gff3</a><br>
</div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
<a href="http://genomes.ua.edu/Kocot/2021-09-10_Maker/protein_alignments.gff3" id="gmail-m_-3511602195170912938LPlnk159063" target="_blank">http://genomes.ua.edu/Kocot/2021-09-10_Maker/protein_alignments.gff3</a></div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
<br>
</div>
<div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
Is there a standalone tool or Maker feature I'm not seeing that can assess whether a gff3 file is correctly formatted for Maker?</div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
<br>
</div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
Thank you again!</div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
Kevin<br>
</div>
<hr style="display:inline-block;width:98%">
<div id="gmail-m_-3511602195170912938divRplyFwdMsg" dir="ltr"><font style="font-size:11pt" face="Calibri, sans-serif" color="#000000"><b>From:</b> Jacques Dainat <<a href="mailto:jacques.dainat@nbis.se" target="_blank">jacques.dainat@nbis.se</a>><br>
<b>Sent:</b> Wednesday, September 22, 2021 2:09 PM<br>
<b>To:</b> Kevin Kocot <<a href="mailto:kmkocot@ua.edu" target="_blank">kmkocot@ua.edu</a>><br>
<b>Cc:</b> <a href="mailto:maker-devel@yandell-lab.org" target="_blank">maker-devel@yandell-lab.org</a> <<a href="mailto:maker-devel@yandell-lab.org" target="_blank">maker-devel@yandell-lab.org</a>>; Carson Holt <<a href="mailto:carsonhh@gmail.com" target="_blank">carsonhh@gmail.com</a>><br>
<b>Subject:</b> [EXTERNAL] Re: [maker-devel] Troubleshooting Maker failure</font>
<div> </div>
</div>
<div style="overflow-wrap: break-word;">Hi Kevin,
<div><br>
</div>
<div>About using AGAT (<a href="http://agat_convert_sp_gxf2gxf.pl" target="_blank">agat_convert_sp_gxf2gxf.pl</a>), two reasons to get empty output files.</div>
<div>I) The feature types (3rd column) are not yet handled by AGAT. You can inform AGAT how to deal with it </div>
<div>See <a href="https://nam11.safelinks.protection.outlook.com/?url=https%3A%2F%2Fagat.readthedocs.io%2Fen%2Flatest%2Ftroubleshooting.html%23agat-throws-features-out-because-the-feature-type-is-not-yet-taken-into-account&data=04%7C01%7Ckmkocot%40ua.edu%7C168456deb2c74e62a85f08d97dfca290%7C2a00728ef0d040b4a4e8ce433f3fbca7%7C0%7C0%7C637679346284061013%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=Wc7PNmVtOVuQHd%2BiDYxQJTuXHlkoltF7eJ4Gv1fkoQc%3D&reserved=0" target="_blank">https://agat.readthedocs.io/en/latest/troubleshooting.html#agat-throws-features-out-because-the-feature-type-is-not-yet-taken-into-account</a></div>
<div>II) The features are thrown by AGAT because child feature are missing <span style="color:rgb(0,0,0)">(e.g. gene feature expect at least one transcript linked to it)</span>. See
<a href="https://nam11.safelinks.protection.outlook.com/?url=https%3A%2F%2Fagat.readthedocs.io%2Fen%2Flatest%2Ftroubleshooting.html%23agat-throws-features-out-because-child-features-are-not-provided&data=04%7C01%7Ckmkocot%40ua.edu%7C168456deb2c74e62a85f08d97dfca290%7C2a00728ef0d040b4a4e8ce433f3fbca7%7C0%7C0%7C637679346284071013%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=UBEc%2BdXAtr%2FOLaJnf5RCMwrp8U3RprkDyPBNDR08ORo%3D&reserved=0" target="_blank">
https://agat.readthedocs.io/en/latest/troubleshooting.html#agat-throws-features-out-because-child-features-are-not-provided</a></div>
<div><br>
</div>
<div>I invite you to open an issue in the AGAT GitHub repository.</div>
<div><br>
</div>
<div>Once the file is parsed correctly you can use the script <a href="http://agat_sp_alignment_output_style.pl" target="_blank">agat_sp_alignment_output_style.pl</a> to turn level1 feature types (e.g. gene) and level2 feature types (e.g. mRNA) into match and match_part features respectively as it can be preferred by
MAKER.</div>
<div><br>
</div>
<div>
<div>
<div dir="auto" style="color:rgb(0,0,0);letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none">
<div dir="auto" style="color:rgb(0,0,0);letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none">
<div>Best regards,</div>
<div><br>
Jacques Dainat, Ph.D.<br>
<br>
</div>
</div>
</div>
</div>
<div><br>
<blockquote type="cite">
<div>On 22 Sep 2021, at 18:21, Carson Holt <<a href="mailto:carsonhh@gmail.com" target="_blank">carsonhh@gmail.com</a>> wrote:</div>
<br>
<div>
<div style="overflow-wrap: break-word;">I’d have to see the GFF, but in general you should organize sequence alignments as match/match_part features.
<div><br>
</div>
<div>Here is n example from the GFF3 format specification:</div>
<div><br>
</div>
<div>
<div><font face="Courier">ctg123 . cDNA_match 1200 9000 . . . ID=cDNA00001</font></div>
<div><font face="Courier">ctg123 . match_part 1200 3200 2.2e-30 + . ID=match00002;Parent=cDNA00001;Target=mjm1123.5 5 506;Gap=M301 D1499 M201</font></div>
<div><font face="Courier">ctg123 . match_part 7000 9000 7.4e-32 - . ID=match00003;Parent=cDNA00001;Target=mjm1123.3 1 502;Gap=M101 D1499 M401</font></div>
<div><br>
</div>
<div>Also make sure you are not inadvertently using GFF2 or GTF. They are not backwards compatible with GFF3.</div>
<div><br>
</div>
<div>—Carson</div>
<div><br>
</div>
<div><br>
<blockquote type="cite">
<div>On Sep 22, 2021, at 8:06 AM, Kevin Kocot <<a href="mailto:kmkocot@ua.edu" target="_blank">kmkocot@ua.edu</a>> wrote:</div>
<br>
<div>
<div style="font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt">
Thanks Carson,</div>
<div style="font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt">
<br>
</div>
<div style="font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt">
I think I see the problem now. Here's what I'm getting:</div>
<div style="font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt">
<br>
</div>
<div style="font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt">
-----<br>
</div>
<div style="font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt">
STATUS: Parsing control files...
<div>STATUS: Processing and indexing input FASTA files...</div>
<div>STATUS: Setting up database for any GFF3 input...</div>
<div>A data structure will be created for you at:</div>
<div>/home/wirenia/Desktop/2021-08-11_MAKER_Dreissena_rostriformis/PGA_assembly_shortened_headers.fasta.maker.output/PGA_assembly_shortened_headers.fasta_datastore</div>
<div><br>
</div>
<div>To access files for individual sequences use the datastore index:</div>
<div>/home/wirenia/Desktop/2021-08-11_MAKER_Dreissena_rostriformis/PGA_assembly_shortened_headers.fasta.maker.output/PGA_assembly_shortened_headers.fasta_master_datastore_index.log</div>
<div><br>
</div>
<div>STATUS: Now running MAKER...</div>
<div>examining contents of the fasta file and run log</div>
<div><br>
</div>
<div><br>
</div>
<div><br>
</div>
<div>--Next Contig--</div>
<div><br>
</div>
<div>Processing run.log file...</div>
<div>#---------------------------------------------------------------------</div>
<div>Now retrying the contig!!</div>
<div>SeqID: PGA_scaffold0</div>
<div>Length: 141759199</div>
<div>Tries: 5!!</div>
<div>#---------------------------------------------------------------------</div>
<div><br>
</div>
<div><br>
</div>
<div>setting up GFF3 output and fasta chunks</div>
<div>prepare section files</div>
<div>Gathering GFF3 input into hits - chunk:0</div>
<div>ERROR: Non-unique top level ID for match.19561.56</div>
<div>While this is technically legal in GFF3, it usually</div>
<div>indicates a poorly fomatted GFF3 file (perhaps you</div>
<div>tried to merge two GFF3 files without accounting for</div>
<div>unique IDs). MAKER will not handle these correctly.</div>
<div><br>
</div>
<div>--> rank=NA, hostname=wirenia</div>
<div>ERROR: Failed while prepare section files</div>
<div>ERROR: Chunk failed at level:12, tier_type:3</div>
<div>FAILED CONTIG:PGA_scaffold0</div>
<div><br>
</div>
<div>ERROR: Chunk failed at level:4, tier_type:0</div>
<div>FAILED CONTIG:PGA_scaffold0</div>
<div><br>
</div>
<div>examining contents of the fasta file and run log</div>
<span></span>-----</div>
<div style="font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt">
<br>
</div>
<div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none">
It looks like maker doesn't like the format of the exonerate gff3 I am using. I tried 'fixing' it with <a href="http://agat_convert_sp_gxf2gxf.pl" target="_blank">agat_convert_sp_gxf2gxf.pl</a>, which seemed to work on my Braker output, but that just produced an empty gff3 file for both my exonerate and PASA gff3 files.
Any advice on how to prepare exonerate or PASA gff3 files for Maker?<br>
</div>
<div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none">
<br>
</div>
<div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none">
Thanks!</div>
<div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none">
Kevin<br>
<hr style="display:inline-block;width:2044.27px">
<div id="gmail-m_-3511602195170912938x_divRplyFwdMsg" dir="ltr"><font style="font-size:11pt" face="Calibri, sans-serif"><b>From:</b><span> </span>Carson Holt <<a href="mailto:carsonhh@gmail.com" target="_blank">carsonhh@gmail.com</a>><br>
<b>Sent:</b><span> </span>Monday, September 20, 2021 8:06 AM<br>
<b>To:</b><span> </span>Kevin Kocot <<a href="mailto:kmkocot@ua.edu" target="_blank">kmkocot@ua.edu</a>><br>
<b>Cc:</b><span> </span><a href="mailto:maker-devel@yandell-lab.org" target="_blank">maker-devel@yandell-lab.org</a><span> </span><<a href="mailto:maker-devel@yandell-lab.org" target="_blank">maker-devel@yandell-lab.org</a>><br>
<b>Subject:</b><span> </span>[EXTERNAL] Re: [maker-devel] Troubleshooting Maker failure</font>
<div> </div>
</div>
<div dir="auto">Hi Kevin,
<div><br>
</div>
<div>The files are already being skipped because of previous failures. Can you increase the try count (-t on the command line) to something like 6, and send me the STDERR after it generates a new failure.</div>
<div><br>
</div>
<div>—Carson <br>
<br>
<div dir="ltr">Sent from my iPhone</div>
<div dir="ltr"><br>
<blockquote type="cite">On Sep 20, 2021, at 4:56 AM, Kevin Kocot <<a href="mailto:kmkocot@ua.edu" target="_blank">kmkocot@ua.edu</a>> wrote:<br>
<br>
</blockquote>
</div>
<blockquote type="cite">
<div dir="ltr">
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt">
Thanks Carson! I've uploaded that file here:</div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt">
<a href="http://genomes.ua.edu/Kocot/2021-09-10_Maker/round1_run_maker_try2.log" id="gmail-m_-3511602195170912938LPlnk439129" target="_blank">http://genomes.ua.edu/Kocot/2021-09-10_Maker/round1_run_maker_try2.log</a><br>
</div>
<div>
<div><br>
</div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt">
<br>
<hr style="display:inline-block;width:2034.47px">
<b>From:</b><span> </span>Carson Holt<br>
<b>Sent:</b><span> </span>Tuesday, September 14, 2021 9:51 PM<br>
<b>To:</b><span> </span>Kevin Kocot<br>
<b>Cc:</b><span> </span><a href="mailto:maker-devel@yandell-lab.org" target="_blank">maker-devel@yandell-lab.org</a><br>
<b>Subject:</b><span> </span>[EXTERNAL] Re: [maker-devel] Troubleshooting Maker failure
<div><br>
</div>
</div>
<div>
<div style="overflow-wrap: break-word;">What I really need is the captured STDERR from the failed run.
<div><br>
</div>
<div>—Carson<br>
<div><br>
<blockquote type="cite">
<div>On Sep 10, 2021, at 9:19 AM, Kevin Kocot <<a href="mailto:kmkocot@ua.edu" rel="noopener noreferrer" target="_blank">kmkocot@ua.edu</a>> wrote:</div>
<br>
<div>
<div style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none">
<div style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif">
<span style="font-size:12pt">Hi Carson and all,</span></div>
<div style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif">
<span style="font-size:12pt"> </span></div>
<div style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif">
<span style="font-size:12pt">I ran Maker 3.01.03 on a chromosome-level mollusc genome assembly using some evidence I previously generated with Funannotate and BRAKER2, but Maker is not completing successfully. Every scaffold in the datastore_index.log
file has both STARTED and FAILED statuses. I can't figure out where the problem lies, though. Running ./Build status indicates all the dependencies are there (I’m not using MPI). The run.log files just ends with "DIED RANK 0" and "DIED COUNT 3." Is there a
way to tell which (if any) of the dependencies is misbehaving here (they all seem to run fine independently) or if my evidence .gff3 files are not correctly formatted?</span></div>
<div style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif">
<span style="font-size:12pt"> </span></div>
<div style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif">
<span style="font-size:12pt">I’ve uploaded a<span><span> </span>zipped sample output folder as well as my config files</span><span> </span>and the datastore.log file here:<span> </span><a href="http://genomes.ua.edu/Kocot/2021-09-10_Maker/" rel="noopener noreferrer" style="color:rgb(5,99,193);text-decoration:underline" target="_blank">http://genomes.ua.edu/Kocot/2021-09-10_Maker/</a><span></span></span></div>
<div style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif">
</div>
<div style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif">
<span style="font-size:12pt">Any guidance on what might be the issue would be greatly appreciated.</span></div>
<div style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif">
<span style="font-size:12pt"> </span></div>
<div style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif">
<span style="font-size:12pt">Thanks!</span></div>
<div style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif">
<span style="font-size:12pt">Kevin</span></div>
<div style="margin:0in;font-size:11pt;font-family:Calibri,sans-serif">
</div>
<p style="margin:7.5pt 0in 0in;font-size:11pt;font-family:Calibri,sans-serif;background-color:white">
<b><span style="font-size:12pt;font-family:Helvetica,sans-serif;color:rgb(153,0,0)">Kevin M. Kocot<br>
</span></b><span style="font-size:8pt;font-family:Helvetica,sans-serif">he/him/his</span><b><span style="font-size:12pt;font-family:Helvetica,sans-serif;color:rgb(153,0,0)"></span></b></p>
<p style="margin:0in 0in 7.5pt;font-size:11pt;font-family:Calibri,sans-serif;background-color:white">
<span style="font-size:11.5pt;font-family:Helvetica,sans-serif">Associate Professor & Curator of Invertebrates<br>
Department of Biological Sciences & Alabama Museum of Natural History<br>
</span><span style="font-size:10.5pt;font-family:Helvetica,sans-serif"><a href="https://www.ua.edu/" rel="noopener noreferrer" style="color:rgb(5,99,193);text-decoration:underline" target="_blank"><span style="color:maroon;text-decoration:none">The
University of Alabama</span></a> <br>
307 Mary Harmon Bryant Hall<br>
Box 870344<br>
Tuscaloosa, AL 35487<br>
phone <a href="tel:205-348-4052" rel="noopener noreferrer" style="color:rgb(5,99,193);text-decoration:underline" target="_blank"><span>205-348-4052</span></a><span> </span></span><span style="font-size:10.5pt;font-family:Helvetica,sans-serif;color:maroon">|</span><span style="font-size:10.5pt;font-family:Helvetica,sans-serif"><span> </span>fax
205-348-4039 <br>
<a href="mailto:kmkocot@ua.edu" rel="noopener noreferrer" style="color:rgb(5,99,193);text-decoration:underline" target="_blank"><span>kmkocot@ua.edu</span></a> </span><span style="font-size:10.5pt;font-family:Helvetica,sans-serif;color:maroon">|</span><span style="font-size:10.5pt;font-family:Helvetica,sans-serif"> <a href="https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.kocotlab.com%2F&data=04%7C01%7Ckmkocot%40ua.edu%7C168456deb2c74e62a85f08d97dfca290%7C2a00728ef0d040b4a4e8ce433f3fbca7%7C0%7C0%7C637679346284081007%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=IPRtDsChZSGeknM5I9qWvgWNHbRQc9a3NREKARlkxY4%3D&reserved=0" rel="noopener noreferrer" target="_blank"><span>www.kocotlab.com</span></a><br>
</span><span style="font-family:Helvetica,sans-serif"><a href="https://uasystem.zoom.us/j/3755490727" rel="noopener noreferrer" style="color:rgb(5,99,193);text-decoration:underline" target="_blank"><span>https://uasystem.zoom.us/j/3755490727</span></a></span></p>
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<span style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;float:none;display:inline">maker-devel
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<a href="mailto:maker-devel@yandell-lab.org" rel="noopener noreferrer" style="color:rgb(5,99,193);text-decoration:underline;font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px" target="_blank">maker-devel@yandell-lab.org</a><br style="font-family:Helvetica;font-size:12px;font-style:normal;font-variant-caps:normal;font-weight:normal;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none">
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_______________________________________________<br>
maker-devel mailing list<br>
<a href="mailto:maker-devel@yandell-lab.org" target="_blank">maker-devel@yandell-lab.org</a><br>
<a href="http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org" target="_blank">http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org</a><br>
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</div></blockquote></div></blockquote></div><br clear="all"><br>-- <br><div dir="ltr" class="gmail_signature">Jacques Dainat<br></div></div>