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<font face="ComicSansMS" class="" style="font-family: ComicSansMS;">Sorry to say probably not a new issue. </font><font face="ComicSansMS" class="" style="font-family: ComicSansMS;">I saw that MAKER is an easy-to-use genome annotation pipeline </font><span style="caret-color: rgb(34, 34, 34); color: rgb(34, 34, 34); background-color: rgb(255, 255, 255);" class="">designed
to be usable by small research groups with little bioinformatics experience. That statement describes me to a t.
</span><span style="font-family: ComicSansMS;" class=""> </span><font face="ComicSansMS" class="" style="font-family: ComicSansMS;">I am new to Whole Genome Sequencing and associated analysis, and have a limited skill set with computers. </font><font face="ComicSansMS" class="" style="font-family: ComicSansMS;">I
have a </font><span style="font-family: ComicSansMS;" class="">a 1.52 GB, 75k unitigs, non-photosynthetic vascular plant assembly for which I would like to predict functional genes. </span>I have tried to follow the tutorial that you have posted for Winter
School 2018 to see if I can duplicate your installation and implementation for my HPC. <font face="ComicSansMS" class="" style="font-family: ComicSansMS;">I doubt that you will be impressed, but I recently received a “certificate” for completing our HPC Linux
course path the University of Wyoming. </font><font face="ComicSansMS" class="">. I realize now how much I don’t know and how much work it will take to complete an annotation, but I am needing to generate at least some preliminary data to include in an upcoming
grant submittal (~2 months). </font></p>
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<div class=""> I read your MAKER wiki page, but it seems that it hasn’t been completed in many cases, with many important parts (important parts to me, in any case) under construction.</div>
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<div class="" style="font-family: ComicSansMS;">I am writing to get any help I can to find a way forward in my project. Any suggestions and any help you can provide would be gratefully accepted!</div>
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<span class="">Sincerely,</span><br class="">
<span class="">Steve Miller</span><br class="">
<span class="">Botany</span><br class="">
<span class="">University of Wyoming</span></p>
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