<html><head><meta http-equiv="content-type" content="text/html; charset=utf-8"></head><body style="overflow-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">It’s looking for _AED= attribute for each mRNA feature. If the other file does not have that set, then it will be empty, and you get a divide by 0 error in the CDF calculation.<div><br></div><div>To get AED into the other GFF3 file, you will have to let MAEKR add it. Give the other file to model_gff along with evidence you want to use (either as pasta files or protein/est off files) and let MAKER calculate AED and add it to your GFF.<br><div><br></div><div>—Carson</div><div><br id="lineBreakAtBeginningOfMessage"><div><br><blockquote type="cite"><div>On Sep 27, 2023, at 10:37 AM, Cerda, Juan Daniel <jdc81@psu.edu> wrote:</div><br class="Apple-interchange-newline"><div><meta charset="UTF-8"><div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;"><span class="ContentPasted0" style="font-size: 12pt; margin: 0px; background-color: rgb(255, 255, 255);">Hello,</span></div><div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;"><div style="font-size: 12pt; margin: 0px; background-color: rgb(255, 255, 255);"><br class="ContentPasted0"></div><div class="ContentPasted0" style="font-size: 12pt; margin: 0px; background-color: rgb(255, 255, 255);">I am trying to compare annotations with some gff file not created by maker, but ever time run the AED script with non-Maker annotations i get the following error:<div style="margin: 0px;"><br class="ContentPasted0"></div><div class="ContentPasted0" style="margin: 0px;">Illegal division by zero at /scratch/users/juan/biotools/AED_cdf_generator.pl line 49.</div><div style="margin: 0px;"><br class="ContentPasted0"></div><div class="ContentPasted0" style="margin: 0px;">I am hoping you can help out with this. Any ideas why this happens?</div><div style="margin: 0px;"><br class="ContentPasted0"></div>Thanks!</div><br></div><div class="elementToProof" style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;"><div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;"><br></div><div id="Signature"><div><div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;">--<div dir="ltr"><div dir="ltr"><span style="font-family: Calibri, Helvetica, sans-serif; font-size: 12pt;">Juan D. Cerda</span><div><span style="font-family: Calibri, Helvetica, sans-serif; font-size: 12pt;">Graduate Student in </span><span style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;">Bioinformatics and Genomics</span></div><div><span style="font-family: Calibri, Helvetica, sans-serif; font-size: 12pt;">Huck Institute of the Life Sciences</span></div><div><span style="font-family: Calibri, Helvetica, sans-serif; font-size: 12pt;">Pennsylvania State University</span></div><span><span style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;">State College, Pennsylvania</span></span></div></div><span id="cid:24473bc3-f364-4cac-b1c4-ea589afdf26a"><Outlook-kvmm4iq1.png></span><br></div></div></div></div><span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;">_______________________________________________</span><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;"><span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;">maker-devel mailing list</span><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;"><a href="mailto:maker-devel@yandell-lab.org" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;">maker-devel@yandell-lab.org</a><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;"><a href="http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;">http://yandell-lab.org/mailman/listinfo/maker-devel_yandell-lab.org</a></div></blockquote></div><br></div></div></body></html>