[maker-devel] maker-2.24 fails while preparing evidence clusters

Markus Ankenbrand markus.ankenbrand at stud-mail.uni-wuerzburg.de
Thu Jun 28 08:03:20 MDT 2012


Hi all,

I'm using maker-2.24 to annotate contigs of a procaryotic organism. I'm
only using coding sequences of a reference species as "altest". Without
any obvious reason annotation fails for some contigs. It seems to have
nothing to do with the contig length. I also tried to run maker with
different reference ESTs and GeneMark. Depending on the setup different
contigs fail.

In case of failure, the log is:
...
cleaning clusters....
total clusters:3 now processing 0
   ...processing 0 of 3
   ...processing 1 of 3
total clusters:3 now processing 0
total clusters:3 now processing 0
   ...processing 0 of 2
Can't call method "start" on an undefined valueERROR: Failed while
preparing evidence clusters for annotations
ERROR: Chunk failed at level:0, tier_type:3
FAILED CONTIG:contig1

ERROR: Chunk failed at level:7, tier_type:0
FAILED CONTIG:contig1

--Next Contig--
...

If someone has any idea how to solve this problem, help would be very
much apriciated.

Greetings,
Markus




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