[maker-devel] Consensus gene models

Eduardo Larriba larriba.ed at gmail.com
Fri May 25 10:01:41 MDT 2012


Hi Carson and people,
I am working on structural annotation of a filamentous fungus, of which
there is little evidence as EST or Protein. For generate consensus gene  based
on limited evidences to me I used  Marker.
For this I created the files GeneMark prediction-is and SNAP.
I run maker using the EST of my organims (85), along with 5700 EST of the
closed organims. I have made ​​predictions with Augustus, and SNAP GeneMark,
with the training files for my organims, in Maker pipeline. Everything works
fine.
My problem is that when I get the consensus sequences of all my contigs,
fasta_merge script (included in Maker), I get different list for each
predictor, as well as when I try to get the gff of all.
They could tell me how I can use Maker consensus for a list of genes? Or I
have to do it manually? There is the possibility that Maker evaluates the
accuracy of each prediction and confirm, so just get a list of the different
predictions?

Thank you very much.


-- 
Eduardo Larriba Tornel
Universidad de Alicante.
Lab. Fitopatología
Dept. Ciencias del Mar y Biología Aplicada
Pabellón 13.
San Vicente del Raspeig
Tel. 96 590 3400 ext 3280
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