[maker-devel] Failed while polishig ESTs

Parul Kudtarkar parulk at caltech.edu
Wed Oct 3 20:14:36 MDT 2012


I am running maker on example data that comes along with installation and
is cited at http://gmod.org/wiki/MAKER_Tutorial_2012#Note

Please advice on the aforementioned error.

---------------------
Maker is now finished!!!

re reading blast report.
/usr/local/maker/test/dpp_contig.maker.output/dpp_contig_datastore/05/1F/contig-dpp-500-500//theVoid.contig-dpp-500-500/contig-dpp-500-500.0.te_proteins%2Efasta.repeatrunner
deleted:0 hits
 in cluster:shadow cluster...
    i_size:0 j_size:0
 sorting hits in shadow cluster...
... finished.
re reading blast report.
/usr/local/maker/test/dpp_contig.maker.output/dpp_contig_datastore/05/1F/contig-dpp-500-500//theVoid.contig-dpp-500-500/contig-dpp-500-500.0.dpp_est%2Efasta.blastn
deleted:-1 hits
re reading blast report.
/usr/local/maker/test/dpp_contig.maker.output/dpp_contig_datastore/05/1F/contig-dpp-500-500//theVoid.contig-dpp-500-500/contig-dpp-500-500.0.dpp_protein%2Efasta.blastx
WARNING: Multiple MAKER processes have been started in the
same directory.

deleted:0 hits
WARNING: Cannot find> dpp-mRNA-4, trying to re-index the fasta.
stop here:dpp-mRNA-4
ERROR: Fasta index error

FATAL ERROR
Maker is now finished!!!

re reading blast report.
/usr/local/maker/test/dpp_contig.maker.output/dpp_contig_datastore/05/1F/contig-dpp-500-500//theVoid.contig-dpp-500-500/contig-dpp-500-500.0.te_proteins%2Efasta.repeatrunner
deleted:0 hits
 in cluster:shadow cluster...
    i_size:0 j_size:0
 sorting hits in shadow cluster...
... finished.
re reading blast report.
/usr/local/maker/test/dpp_contig.maker.output/dpp_contig_datastore/05/1F/contig-dpp-500-500//theVoid.contig-dpp-500-500/contig-dpp-500-500.0.dpp_est%2Efasta.blastn
deleted:-1 hits
re reading blast report.
/usr/local/maker/test/dpp_contig.maker.output/dpp_contig_datastore/05/1F/contig-dpp-500-500//theVoid.contig-dpp-500-500/contig-dpp-500-500.0.dpp_protein%2Efasta.blastx
WARNING: Multiple MAKER processes have been started in the
same directory.

deleted:0 hits
WARNING: Cannot find> dpp-mRNA-4, trying to re-index the fasta.
stop here:dpp-mRNA-4
ERROR: Fasta index error

FATAL ERROR
ERROR: Failed while polishig ESTs!!

ERROR: Chunk failed at level 14
!!
FAILED CONTIG:contig-dpp-500-500



ERROR: Chunk failed at level 14
!!
FAILED CONTIG:contig-dpp-500-500
------------------------------------


Many thanks,
Parul Kudtarkar

--
Scientific Programmer
Center for Computational Regulatory Genomics
Beckman Institute,
California Institute of Technology
http://www.spbase.org





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