[maker-devel] Maker not outputting exons with EST and Protein Evidence

Innocent Onsongo onson001 at umn.edu
Mon Jul 8 16:12:06 MDT 2013


Maker Developers,

I am testing Maker on a well annotated contig from the encode project and
in one of the genes, maker does not output an exon despite HSPs supporting
it. I have attached a compressed folder with

ENr122_genes.gff: The validated genes as per encode
ENr122.gff: Makers output
maker_opts.ctl: The control file I used
ENr122_98669-134249.pptx: A power point visually illustrated the exon in
question.

I even set the parameters est2genome and protein2genome to 1 but still
Maker did not output this exon.

Is there a good reason Maker does no have this exon in its final model
despite the HSPs supporting it?

Thanks,
Getiria

-- 
Getiria Onsongo, Ph.D.
Informatics Analyst, Research Informatics Support System
Minnesota Supercomputing Institute for Advanced Computational Research
University of Minnesota
Minneapolis, MN 55455
Phone: 612-624-0532
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