[maker-devel] RNA-seq data for MAKER2

[Carson Holt]

As far as total depth I don't know, but I'd recommend using something like
trinity to assemble the reads and supply them to the est= option in fasta
format.

--Carson



On 7/10/13 10:27 PM, "yuharas at takara-bio.co.jp" <yuharas at takara-bio.co.jp>
wrote:

>Dear developers
>
>I'm considering using MAKER2 to annotate plant genome (~1Gb) I've
>assembled using illumina HiSeq data.
>And I'm planning to obtain mRNA-seq data by using HiSeq.
>Do you have any recommendation of sequencing depth of RNA-seq data for
>using MAKER2 annotation pipeline?
>
>Thanks,
>Satoshi
>
>
>_______________________________________________
>maker-devel mailing list
>maker-devel at box290.bluehost.com
>http://box290.bluehost.com/mailman/listinfo/maker-devel_yandell-lab.org