[maker-devel] Testing MAKER After Installation

Cexzur Jimenez Jr. cexzurjimenezjr at gmail.com
Thu Feb 6 22:27:13 MST 2014


Hello,

I have finished installing MAKER marked by "PERL Dependencies: INSTALLED,
External Programs: INSTALLED, MPI SUPPORT: NOT CONFIGURED,
MAKER: INSTALLED" and it seems everything's fine. I'm using MAKER 2.10 and
I have followed the installation instructions both in its corresponding
"README" and "INSTALL" files and the 2012 GMOD MAKER Tutorial. After
editing the three configuration files and run with "maker", I saw the
following error in my terminal. I have searched Google and tried the
solutions offered there but the error is still showing. Below is the error
I got:


Can't locate package GDBM_File for @AnyDBM_File::ISA at
/usr/lib/perl/5.14/DB_File.pm line 287.
Can't locate package NDBM_File for @AnyDBM_File::ISA at
/usr/lib/perl/5.14/DB_File.pm line 287.
Can't locate package SDBM_File for @AnyDBM_File::ISA at
/usr/lib/perl/5.14/DB_File.pm line 287.
A data structure will be created for you at:
/home/cexzurjimenezjr/Documents/data/dpp_contig.maker.output/dpp_contig_datastore

To access files for individual sequences use the datastore index:
/home/cexzurjimenezjr/Documents/data/dpp_contig.maker.output/dpp_contig_master_datastore_index.log




--Next Contig--

#---------------------------------------------------------------------
Now starting the contig!!
SeqID: contig-dpp-500-500
Length: 32156
#---------------------------------------------------------------------


running  repeat masker.
#--------- command -------------#
Widget::RepeatMasker:
/usr/local/maker/exe/RepeatMasker/RepeatMasker
/home/cexzurjimenezjr/Documents/data/dpp_contig.maker.output/dpp_contig_datastore/05/1F/contig-dpp-500-500//theVoid.contig-dpp-500-500/contig-dpp-500-500.0.all.rb
-species all -dir
/home/cexzurjimenezjr/Documents/data/dpp_contig.maker.output/dpp_contig_datastore/05/1F/contig-dpp-500-500//theVoid.contig-dpp-500-500
-pa 1
#-------------------------------#
Building general libraries in:
/usr/local/maker/exe/RepeatMasker/Libraries/20120418/general
RepeatMasker::createLib(): Error invoking /usr/local/blast/bin/makeblastdb
on file /usr/local/maker/exe/RepeatMasker/Libraries/20120418/general/at.lib.
ERROR: RepeatMasker failed

FATAL ERROR
ERROR: Failed while doing repeat masking!!

ERROR: Chunk failed at level 2
!!
FAILED CONTIG:contig-dpp-500-500




--Next Contig--

Processing run.log file...
MAKER WARNING: The file
dpp_contig.maker.output/dpp_contig_datastore/05/1F/contig-dpp-500-500//theVoid.contig-dpp-500-500/contig-dpp-500-500.0.all.rb.out
did not finish on the last run and must be erased
#---------------------------------------------------------------------
Now retrying the contig!!
SeqID: contig-dpp-500-500
Length: 32156
Retry: 1!!
#---------------------------------------------------------------------


running  repeat masker.
#--------- command -------------#
Widget::RepeatMasker:
/usr/local/maker/exe/RepeatMasker/RepeatMasker
/home/cexzurjimenezjr/Documents/data/dpp_contig.maker.output/dpp_contig_datastore/05/1F/contig-dpp-500-500//theVoid.contig-dpp-500-500/contig-dpp-500-500.0.all.rb
-species all -dir
/home/cexzurjimenezjr/Documents/data/dpp_contig.maker.output/dpp_contig_datastore/05/1F/contig-dpp-500-500//theVoid.contig-dpp-500-500
-pa 1
#-------------------------------#
Building general libraries in:
/usr/local/maker/exe/RepeatMasker/Libraries/20120418/general
RepeatMasker::createLib(): Error invoking /usr/local/blast/bin/makeblastdb
on file /usr/local/maker/exe/RepeatMasker/Libraries/20120418/general/at.lib.
ERROR: RepeatMasker failed

FATAL ERROR
ERROR: Failed while doing repeat masking!!

ERROR: Chunk failed at level 2
!!
FAILED CONTIG:contig-dpp-500-500




--Next Contig--

Processing run.log file...
MAKER WARNING: The file
dpp_contig.maker.output/dpp_contig_datastore/05/1F/contig-dpp-500-500//theVoid.contig-dpp-500-500/contig-dpp-500-500.0.all.rb.out
did not finish on the last run and must be erased


Maker is now finished!!!


Can you state to me the error and what part of the installation did I go
wrong? Your help will be very much appreciated. Thank you.

Attached herein are configuration files I used for MAKER.


Sincerely,

CJ
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