[maker-devel] Failed while doing blastx of proteins

[Sok Lim Chew]

Hi all,

The following errors occurred while I was using MAKER for annotation. I
have searched around this forum but seems like the solutions provided do
not works for me.

#################################################################

------------- EXCEPTION: Bio::Root::Exception -------------

MSG: Must have defined a valid name for Hit

STACK: Error::throw

STACK: Bio::Root::Root::throw /usr/local/share/perl5/Bio/Root/Root.pm:486

STACK: Bio::Search::Hit::GenericHit::new
/usr/local/share/perl5/Bio/Search/Hit/GenericHit.pm:149

STACK: Bio::Search::Hit::PhatHit::Base::new
maker/bin/../lib/Bio/Search/Hit/PhatHit/Base.pm:127

STACK: Bio::Search::Hit::PhatHit::blastx::new
maker/bin/../lib/Bio/Search/Hit/PhatHit/blastx.pm:125

STACK: Bio::Search::Hit::HitFactory::create
/usr/local/share/perl5/Bio/Search/Hit/HitFactory.pm:124

STACK: Bio::Factory::ObjectFactoryI::create_object
/usr/local/share/perl5/Bio/Factory/ObjectFactoryI.pm:114

STACK: Bio::Search::Iteration::GenericIteration::newhits_below_threshold
/usr/local/share/perl5/Bio/Search/Iteration/GenericIteration.pm:506

STACK: Bio::Search::Iteration::GenericIteration::newhits
/usr/local/share/perl5/Bio/Search/Iteration/GenericIteration.pm:488

STACK: Bio::Search::Iteration::GenericIteration::hits
/usr/local/share/perl5/Bio/Search/Iteration/GenericIteration.pm:469

STACK: Bio::Search::Result::BlastResult::hits
/usr/local/share/perl5/Bio/Search/Result/BlastResult.pm:168

STACK: Bio::Search::Result::BlastResult::num_hits
/usr/local/share/perl5/Bio/Search/Result/BlastResult.pm:242

STACK: Widget::blastx::keepers

maker/bin/../lib/Widget/blastx.pm:164

STACK: Widget::blastx::parse

maker/bin/../lib/Widget/blastx.pm:132

STACK: GI::blastx_as_chunks

maker/bin/../lib/GI.pm:2457

STACK: GI::blastx_as_chunks

maker/bin/../lib/GI.pm:2466

STACK: Process::MpiChunk::_go

maker/bin/../lib/Process/MpiChunk.pm:2687

STACK: Process::MpiChunk::run

maker/bin/../lib/Process/MpiChunk.pm:341

STACK: Process::MpiChunk::run_all

maker/bin/../lib/Process/MpiChunk.pm:357

STACK: Process::MpiTiers::run_all maker/bin/../lib/Process/MpiTiers.pm:287

STACK: Process::MpiTiers::run_all maker/bin/../lib/Process/MpiTiers.pm:287

STACK: maker/bin/maker:686

-----------------------------------------------------------

--> rank=NA, hostname=gena2

--> rank=NA, hostname=gena2

--> rank=NA, hostname=gena2

--> rank=NA, hostname=gena2

ERROR: Failed while doing blastx of proteins

ERROR: Chunk failed at level:8, tier_type:3

FAILED CONTIG:Contig1



ERROR: Chunk failed at level:4, tier_type:0

FAILED CONTIG:Contig1



examining contents of the fasta file and run log



###########################################################


Is anyone has any idea on this?


Thanks,

SokLim
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