[maker-devel] MAKER/3.00.0-beta: missing some accessory scripts
Gloria Rendon
gloriarendon at gmail.com
Fri Feb 26 15:14:26 MST 2016
Hello,
My name is Gloria Rendon. I work at the Carle Woese Institute for Genomic
Biology at the University of Illinois at Urbana-Champaign.
In recent months we used MAKER/3.00.0-beta to generate annotations (in GFF3
format) for a de-novo assembly that we produced in-house for the Taro plant.
As part of the same project, I need to run now an analysis with RNA seq
data for the same Tarospecies.
I am going to use STAR for the alignment step and I need to provide the
annotations file in GTF format, not in GFF3 format as I currently have.
In order to perform the GFF3->GTF conversion I was planning to run some of
the accessory scripts that come with MAKER
add_utr_start_stop_gff
gff3_2_gtf
However I just noticed that my installation of MAKER is missing those two
scripts.
This is how the MAKER/bin folder looks like now:
$ ls /home/groups/hpcbio/apps/maker/maker-3.00.0-beta/bin/
AED_cdf_generator.pl ipr_update_gff maker_map_ids
cegma2zff iprscan2gff3 map2assembly
chado2gff3 maker map_data_ids
compare_gff3_to_chado maker2chado map_fasta_ids
cufflinks2gff3 maker2eval_gtf map_gff_ids
evaluator maker2jbrowse match2gene.pl
fasta_merge maker2wap quality_filter.pl
fasta_tool maker2zff tophat2gff3
genemark_gtf2gff3 maker_functional_fasta
gff3_merge maker_functional_gff
btw, earlier versions of MAKER that are also installed on our cluster as
also missing those scripts.
Could you please tell me how to remedy the situation?
Do you have executables of the two scripts that you can share with me?
OR
Do I need to re-install MAKER with special configuration options?
Thank you very much for the attention to this matter.
Sincerely,
Gloria
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