[maker-devel] gff file: possible sources

Daniel Ence dence at genetics.utah.edu
Thu Jan 12 06:28:24 MST 2017


Hi Qihua, the cdna2genome is the polished tblastx alignments from Exonerate. Basically, the source column should be the name of the tool that generated the alignment, prediction, or gene model.

~Daniel


Daniel Ence
Graduate Student
Eccles Institute of Human Genetics
University of Utah
15 North 2030 East, Room 2100
Salt Lake City, UT 84112-5330

On Jan 11, 2017, at 11:28 PM, Qihua Liang <qlian003 at ucr.edu<mailto:qlian003 at ucr.edu>> wrote:

Hi Maker develop team!

I am trying to figure the second column of gff file generated by maker, which should be the source of this annotation. Besides of what the tutorial lists as,

Possible Sources Include:

  *   BLASTN - BLASTN alignment of EST evidence
  *   BLASTX - BLASTX alignment of protein evidence
  *   TBLASTX - TBLASTX alignment of EST evidence from closely related organisms
  *   EST2Genome - Polished EST alignment from Exonerate
  *   Protein2Genome - Polished protein alignment from Exonerate
  *   SNAP - SNAP ab inito gene prediction
  *   GENEMARK - GeneMarkab inito gene prediction
  *   Augustus - Augustus ab inito gene prediction
  *   FgenesH - FGENESH ab inito gene prediction
  *   Repeatmasker - RepeatMasker identified repeat
  *   RepeatRunner - RepeatRunner identified repeat from the repeat protein database
  *   tRNAScan - tRNAScan-SE tRNA predictions (coming soon)
  *   PASA - PASA gene predictions (coming soon)

There are other sources that I noticed from my gff file, like cdna2genome. Is there any other detailed documentation explaining such sources besides of those listed above?

Thanks
Qihua

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