[maker-devel] ThrowNullPointerException()

[Marcus Naymik]

I have now tried with multiple versions of blast (2.6 and 2.28 binaries and
built from source) and get the same error:

setting up GFF3 output and fasta chunks

doing blastn of ESTs

running  blast search.

#--------- command -------------#

Widget::blastn:

/home/mnaymik/TOOLS/ncbi-blast-2.2.28+/bin/blastn -db
/scratch/mnaymik/maker/tmp/maker_cah

#-------------------------------#

Error: NCBI C++ Exception:


"/home/mnaymik/TOOLS/ncbi-blast-2.2.28+-src/c++/src/corelib/ncbiobj.cpp",
line 925: Cr


Error: NCBI C++ Exception:


"/home/mnaymik/TOOLS/ncbi-blast-2.2.28+-src/c++/src/corelib/ncbiobj.cpp",
line 925: Cr


examining contents of the fasta file and run log

ERROR: BLASTN failed

--> rank=87, hostname=pnap-pe7-s09

ERROR: Failed while doing blastn of ESTs

ERROR: Chunk failed at level:0, tier_type:3

FAILED CONTIG:6537645


ERROR: BLASTN failed

--> rank=88, hostname=pnap-pe7-s09

ERROR: Failed while doing blastn of ESTs

ERROR: Chunk failed at level:0, tier_type:3

FAILED CONTIG:6537659


ERROR: Chunk failed at level:4, tier_type:0

FAILED CONTIG:6537645


ERROR: Chunk failed at level:4, tier_type:0

FAILED CONTIG:6537659



On Thu, Mar 2, 2017 at 1:25 PM, Carson Holt <carsonhh at gmail.com> wrote:

> Try reinstalling blast, or upgrade to a newer version of blast.
>
> —Carson
>
>
> On Mar 2, 2017, at 1:05 PM, Marcus Naymik <mnaymik at tgen.org> wrote:
>
>
> I have maker running with MPI and I get this error over and over again for
> every contig. Any Ideas?
>
>
> MAKER WARNING: All old files will be erased before continuing
>
> #---------------------------------------------------------------------
>
> Now starting the contig!!
>
> SeqID: 5239
>
> Length: 1395
>
> #---------------------------------------------------------------------
>
>
>
> Error: NCBI C++ Exception:
>
>     "/packages/BUILDS/ncbi-blast-2.2.28+-src/c++/src/corelib/ncbiobj.cpp",
> line 925: Criti
>
>
>
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