[maker-devel] gff3 valiadation with GAG and loss of Name information, start-stop codons

[Giroux, Emily (CFIA/ACIA)]

Hi,

After running my rounds with Maker, I went on to using the Genome Annotation Generator (GAG) and tbl2asn to find errors and fix them. My plan is to run BLAST and InterProScan after so that I don't waste time annotating invalid features. However, when running GAG, all the protein_gff:protein2genome annotations get removed, along with the Name matches that I got to related species. Somehow I feel it would be better to find the errors, and go back to the last Maker generated gff3 instead of continuing on successively with each GAG-cleaned-up gff. However, another thing is that the start and stop codons are not included in the Maker gff, and there is no way that I can fix this all in the original manually.

My question is, is it a real loss to lose the protein_gff:protein2genome information, and all the Name info, or does this get added back once I run BLAST and InterProScan? Does Maker have a function to add the Start and Stop codons? Should I really be running BLAST and InterProScan before going on to GAG? Lastly, MAKER adds tRNA annotations, but for each of these, tbl2asn results in errors: SEQ_FEAT.MissingTrnaAA, and I'm not sure if I can just ignore this...

Thanks,

Emily

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